Abstract
The aim of this research was to evaluate the effect of soil and rhizosphere pH on the mobilisation of Zn by various tobacco genotypes. One-month-old tobacco plants were grown for 8 days on top of a thin layer of an arable soil that had been sampled near a Zn smelter. A range of rhizosphere pH values was obtained either by growing nitrate-fed tobacco on top of the soil amended with various amounts of acid or lime, or by growing tobacco on top of the unamended soil with nitrate or ammonium supply. In the latter case, we used three genotypes that were assumed to differ in their ability to accumulate Zn or acidify the rhizosphere and, hence, mobilise soil Zn. In spite of the moderate level of contamination of the soil, tobacco took up substantial amounts of soil Zn. No difference was found between the three genotypes. Exchangeable Zn steeply increased with decreasing soil pH, which could be adequately modelled with a simple model. Whatever the source of nitrogen supplied, a significant acidification occurred in the rhizosphere. This explains why the observed Zn mobilisation was larger than expected on the basis of bulk soil pH values. Taking account of the change of pH induced by tobacco roots is thus of prime importance for better predicting the actual amount of exchangeable Zn in the rhizosphere and, thereafter the bioavailability of soil Zn.
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References
AOAC 1975 Official Methods of Analysis, 12th edition. Association of Official Analytical Chemists, Washington DC.
AFNOR 1987 Recueil de normes françaises. Qualité des sols, méthodes d'analyse. AFNOR, Paris. 135 pp.
Awad F and Römheld V 2000 Mobilisation of heavy metals from contaminated calcareous soils by plant born, microbial and syn-thetic chelators and their uptake by wheat plants. J. Plant Nutr. 23, 1847–1855.
Barrow N J 1993 Mechanisms of reaction of zinc with soil and soil components. In Zinc in Soils and Plants, Proceedings of the International Symposium held at The University of Western Australia, 27–28 September. Ed. A D Robson. pp. 15–31. Kluwer Academic Publishers, Dordrecht.
Barrow N J and Whelan B R 1998 Comparing the effects of pH on the sorption of metals by soil and by goethite, and on uptake by plants. Eur. J. Soil Sci. 49, 683–692.
Borkert C M and Cox F R 1999 Effects of acidity at high soil zinc, copper, and manganese on peanut, rice, and soybean. Commun. Soil Sci. Plant Anal. 30, 1371–1384.
Boruvka L, Kozak J and Kristoufkova S 1997 Heavy metal accumu-lation in plants grown in heavily polluted soils. Folia Microbiol. 42, 524–526.
Chaignon V, Malta Di and Hinsinger P 2002 Fe-deficiency increases Cu acquisition by wheat cropped in a Cu-contaminated vineyard soil. New Phytol. 15, 4121–130.
Davis-Carter J G and Shuman L M 1993 Influence of texture and pH of kaolinitic soils on zinc fractions and zinc uptake by peanuts. Soil Sci. 155, 376–384.
Ernst W H O, Nelissen H J M and Bookum W M T 2000 Com-bination toxicology of metal-enriched soils physiological responses of a Zn-and Cd-resistant ecotype of Silene vulgaris on polymetallic soils. Environ. Exp. Bot. 43, 55–71.
FAO 1998 Soil Map of the World. FAO, Rome, Italy.
Garcia Sanchez A, Moyano A and Munez C 1999 Forms of cad-mium, lead, and zinc in polluted mining soils and uptake by plants Soria Province, Spain. Commun. Soil Sci. Plant Anal. 30, 1385–1402.
Guivarch A, Hinsinger P and Staunton S 1999 Root uptake and distribution of radiocaesium from contaminated soils and the en-hancement of Cs adsorption in the rhizosphere. Plant Soil 211, 131–138.
Hinsinger P 2001 Bioavailability of trace elements as related to root-induced chemical changes in the rhizosphere. In Trace Ele-ments in the Rhizosphere. Eds. G R Gobran, WW Wenzel and E Lombi. pp. 25–41. CRC Press LCC, Boca Raton, FL.
Hinsinger P, Plassard C, Tang C and Jaillard B 2003 Origins of root-mediated pH changes in the rhizosphere and their responses to environmental contraints – a review. Plant Soil 248, 43–59.
Holm P E, Christensen T H, Tjell J C and McGrath S P 1995 Speciation of cadmium and zinc with application to soil solutions. J. Environ. Qual. 24, 183–190.
Hu G S, Cao Z H, Zhou X R, Li Z L and Zhang X 1993 Behaviour of potassium and trace elements in rhizosphere of flue-cured tobacco Nicotiana tabacum L. Pedosphere 3, 207–220.
Isaure M P, Laboudigue A, Manceau A, Sarret G, Tiffreau C, Tro-cellier P, Lamble G, Hazemann J L and Chateigner D 2002 Quantitative Zn speciaiton in a contaminated dredged sediment by µ-PIXE, µ-SXRF, EXAFS spectroscopy and principal component analysis. Geochim. Cosmochim. Acta 66(9) 1549–1567.
Jaillard B, Plassard C and Hinsinger P 2003 Measurements of H +fluxes and concentrations in the rhizosphere. In Handbook of Soil Acidity. Ed. Z Rengel. pp. 231–266. Marcel Dekker Inc, New York.
Kiekens L 1995 Zinc. In Heavy Metals in Soils. Ed. B J Alloway. pp. 284–305. Blackie Academic & Professional, London.
Krauss M, Wilcke W, Kobza J and Zech W 2002 Predicting heavy metal transfer from soil to plant potential use of Freundlich-type functions. J. Plant Nutr. Soil Sci. 165; 3–8.
Kuchenbuch R 1985 The significance of ion exchange processes in the rhizosphere for the plant availability of potassium. Potash Rev. 84, 1–12.
Lindsay WL 1979 Chemical Equilibria in Soils. John Wiley and Sons Ltd., Chichester, Sussex. 449 pp.
Lorenz S E, Hamon R E, McGrath S P, Holm P E and Christensen T H 1994 Applications of fertilizer cations affect cadmium and zinc concentrations in soil solutions and uptake by plants. Eur. J. Soil Sci. 45, 159–165.
Lorenz S E, Hamon R E, Holm P E, Domingues H C, Sequeira E M, Christensen T H and McGrath S P 1997 Cadmium and zinc in plants and soil solutions from contaminated soils. Plant Soil 189, 21–31.
Luo Y M, Christie P and Baker A J M 2000 Soil solution Zn and pH dynamics in non-rhizosphere soil and in the rhizosphere of Thlaspi caerulescens grown in a Zn/Cd-contaminated soil. Chemosphere 41, 161–164.
Manceau A, Lanson B, Schlegel M L, Hargé J C, Musso M, Eybert-Bérard L, Hazemann J L, Chateigner D and Lamble G M 2000 Quantitative Zn speciation in smelter-contaminated soils by exafs spectroscopy. Am. J. Sci. 300, 289–343.
Marschner H 1995 Mineral nutrition of Higher Plants. 2nd Edn. Academic Press, New York. 889 pp.
Marschner H and Römheld V 1994 Strategies of plants for acquisi-tion of iron. Plant Soil 165, 261–274.
McGrath S P, Shen Z G and Zhao F J 1997 Heavy metal uptake and chemical changes in the rhizosphere of Thlaspi caerulescens and Thlaspi ochroleucum grown in contaminated soils. Plant Soil 188, 153–159.
McGrath S P, Zhao F J and Lombi E 2001 Plant and rhizosphere processes involved in phytoremediation of metal-contaminated soils. Plant Soil 232, 207–214.
Mench M, Vangronsveld J, Didier V and Clijsters H 1994 Eval-uation of metal mobility, plant availability and immobilization.32 by chemical agents in a limed-silty soil. Environ. Pollut. 86, 279–286.
Moriau L, Michelet B, Bogaerts P, Lambert L, Michel A, Oufat-tole M and Boutry M 1999 Expression analysis of two gene subfamilies encoding the plasma membrane H +-ATPase in Nico-tiana plumbaginifolia reveals the major transport functions of this enzyme. Plant J. 19, 31–41.
Msaky J J and Calvet R 1990 Adsorption behavior of copper and zinc in soils influence of pH on adsorption characteristics. Soil Sci. 150, 513–522.
Niebes J F, Hinsinger P, Jaillard B and Dufey J E 1993 Release of nonexchangeable potassium from different size fractions of two highly K-fertilized soils in the rhizosphere of rape Brassica napus cv Drakkar. Plant Soil 155/156, 403–406.
Orsini L and Rémy J C 1976 The use of the chloride of cobalti-hexamine for the simultaneous determination of the exchange capacity and exchangeable bases in soils. Sci. Sol. 4, 269–275.
Rengel Z 1993 Mechanistic simulation models of nutrient uptake a review. Plant Soil 152, 161–173.
Saha J K, Adhikari T and Biswapati M 1999 Effect of lime and or-ganic matter on distribution of zinc, copper, iron, and manganese in acid soils. Commun. Soil Sci. Plant Anal. 30, 1819–1829.
Sanders J R and Kherbawy M I E 1987 The effect of pH on zinc ad-sorption equilibria and exchangeable zinc pools in soils. Environ. Pollut. 44, 165–176.
Sauvé S, Hendershot W and Allen H E 2000 Solid-solution parti-tioning of metals in contaminated soils dependence on pH, total metal burden, and organic matter. Environ. Sci. Technol. 34, 1125–1131.
Shuman L M 1986 Effect of liming on the distribution of man-ganese, copper, iron, and zinc among soil fractions. Soil Sci. Soc. Am. J. 50, 1236–1240.
Sims J T 1986 Soil pH effects on the distribution and plant avail-ability of manganese, copper, and zinc. Soil Sci. Soc. Am. J. 50, 367–373.
Statistica edition 98, Kernel Version 5.1 M, StatSoft France 1984–1998, StatSoft Inc., Tulsa, OK.
Tiller K G, Gerth J and Brummer G 1984 The relative affinities of Cd, Ni and Zn for different soil clay fractions and goethite. Geoderma 34, 17–35.
Tyler G and Olsson T 2001 Concentrations of 60 elements in the soil solution as related to the soil acidity. Eur. J. Soil Sci. 52, 151–165.
Ure A M 1995 Methods of analysis of heavy metals in soils. In Heavy Metals in Soils. pp. 40–80. Ed. B J Alloway. Blackie & Son Ltd, Bishopbriggs, Glasgow.
Vansuyt G, Mench M and Briat J F 2000 Soil dependent variability of leaf iron accumulation in transgenic tobacco over expressing ferritin. Plant Physiol. Biochem. 38, 499–506.
Vansuyt G, Souche G, Straczek A, Briat J-F and Jaillard B 2003 Flux of protons released by wild type and ferritin over-expressor tobacco plants: effects of phosphorus and iron nutrition. Plant Physiol. Biochem. 41, 27–33.
van Wuytswinkel O, Vansuyt G, Grignon N, Fourcroy P and Briat JF 1999 Iron homeostasis alteration in transgenic tobacco over-expressing ferritin. Plant J. 17, 93–97.
Wu X and Aasen I 1994 Models for predicting soil zinc availability for barley. Plant Soil 163, 279–285.
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Loosemore, ¡., Straczek, A., Hinsinger, P. et al. Zinc mobilisation from a contaminated soil by three genotypes of tobacco as affected by soil and rhizosphere pH. Plant and Soil 260, 19–32 (2004). https://doi.org/10.1023/B:PLSO.0000030173.71500.e1
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DOI: https://doi.org/10.1023/B:PLSO.0000030173.71500.e1