Skip to main content
SpringerLink
Log in

Resonance Energy Transfer Between Tryptophan-214 in Human Serum Albumin and Acrylodan, Prodan, and Promen

  • Published:
The Protein Journal Aims and scope Submit manuscript

Abstract

It has been proposed that acrylodan (6-acryloyl-2-dimethylaminonaphthalene) and prodan (6-propionyl-2-dimethylaminonaphthalene) bind to site I of human serum albumin, whereas promen (6-propionyl-2-methoxynaphthalene) binds to site II of this carrier protein. Because human albumin contains only one single tryptophan, at position 214, it has been possible to measure the distances from this amino-acid residue to each of the three probes by nonradiative energy transfer. The distances calculated, 2.97 ± 0.10 nm, 3.14 ± 0.11 nm, and 2.62 ± 0.17 nm, respectively, confirm the locations previously proposed for all three probes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chignell, C. F. (1969). Mol. Pharmacol. 5: 244–252.

    Google Scholar 

  • Catalan, J., Laynez, J., and Garcia Blanco, F. (1991). J. Fluoresc. 1: 215–223.

    Google Scholar 

  • Cortijo, M., Steinberg, I., and Shaltiel, S. (1971). J. Biol. Chem. 246: 933–938.

    Google Scholar 

  • Flora, K., Brennan, J. D., Baker, G. A., Doody, M. A., and Bright, F. V. (1998). Biophys. J. 75: 1084–1096.

    Google Scholar 

  • Förster, T. (1959). Discuss. Faraday Soc. 27: 7–17.

    Google Scholar 

  • González-Jiménez, J. (1994). Chem. Biol. Interact. 91: 65–74.

    Google Scholar 

  • González-Jiménez, J., and Cortijo, M. (2002). J. Protein. Chem. 21: 73–77.

    Google Scholar 

  • González-Jiménez, J., and García-Cantalejo, J. M. (2002). In: Vargas, F.(ed.), Advances in Biomedical Applications of Photochemistry & Photobiology, Kerala, India, pp. 51–78.

    Google Scholar 

  • González-Jiménez, J., Frutos, G., Cayre, I., and Cortijo, M. (1991). Biochemie 73: 551–556.

    Google Scholar 

  • González-Jiménez, J., Jacquotte, H., and Cayre, I. (1992). Chem. Biol. Interact. 84: 221–228.

    Google Scholar 

  • González-Jiménez, J., Moreno, F., and Garcia Blanco, F. (1995a). J. Pharm. Pharmacol. 47: 436–441.

    Google Scholar 

  • González-Jiménez, J., Corrales, S., Moreno, F., and García Blanco, F. (1995b). Chem. Pharm. Bull. (Tokyo) 43: 1949–1952. bib id="bib13">Hagag, N., Birnbraum, E. R., and Darnall, D. W. (1983). Biochemistry 22: 2420–2427.

    Google Scholar 

  • He, X. M., and Carter, D. C. (1992). Nature 358: 209–215.

    Google Scholar 

  • Honor, B., and Pedersen, O. (1989). Biochem. J. 258: 199–204.

    Google Scholar 

  • Kragh-Hansen, U. (1981). Pharmacol. Rev. 33: 17–53.

    Google Scholar 

  • Krag-Hansen, U. (1985). Biochem J. 225: 629–638.

    Google Scholar 

  • Lakowicz, J. R. (1983). Principles of Fluorescence Spectroscopy, Plenum Press, New York.

    Google Scholar 

  • Lakowicz, J. R., and Weber, G. (1980). Biophys. J. 32: 591–601.

    Google Scholar 

  • Llor, J., Sánchez-Ruiz, M., Cortijo, M., and Clares, B. (1984). Anales de Química 80: 701–707.

    Google Scholar 

  • Melhuish, W. H. (1961). J. Phys. Chem. 65: 229–235.

    Google Scholar 

  • Moreno, F., and González-Jiménez, J. (1999). Chem. Biol. Interact. 121: 237–252.

    Google Scholar 

  • Moreno, F., Cortijo, M., and González-Jiménez, J. (1999a). Photochem. Photobiol. 70: 695–700.

    Google Scholar 

  • Moreno, F., Cortijo, M., and González-Jiménez, J. (1999b). Photochem. Photobiol. 69: 8–15.

    Google Scholar 

  • Muller, W. E., and Wollert, U. (1979). Pharmacology 19: 56–59.

    Google Scholar 

  • Parker, C. A. (1968). Photoluminiscence of Solutions, Elsevier, Amsterdam.

    Google Scholar 

  • Peters, T., Jr. (1975). The Plasma Proteins, Vol. 1, Academic Press, New York.

    Google Scholar 

  • Peters, T., Jr. (1996). All About Albumin. Biochemistry, Genetics and Medical Applications, Academic Press, San Diego, CA.

    Google Scholar 

  • Prendergast, F. G., Meyer, M., Carlson, G. L., Iida, S., and Potter, J. D. (1983). J. Biol. Chem. 258: 7541–7544.

    Google Scholar 

  • Siano, D. B. (1972). J. Chem. Educ. 49: 755–757.

    Google Scholar 

  • Sudlow, G., Birkett, D., and Wade, D. N. (1975). Mol. Pharmacol. 11: 824–832.

    Google Scholar 

  • Sugio, S., Kashima, A., Mochizuki, S., Noda, M., and Kobayashi, K. (1999). Protein Eng. 12: 439–446.

    Google Scholar 

  • Suzuhida, M., Le, H. P., Shahid, F., McPherson, R. A., Birnbaum, E. R., and Darnall, D. W. (1983). Biochemistry 22: 2415–2420.

    Google Scholar 

  • Weber, G., and Farris, F. J. (1979). Biochemistry 18: 3075–3078.

    Google Scholar 

Download references

Authors
  1. José González-Jiménez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manuel Cortijo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

González-Jiménez, J., Cortijo, M. Resonance Energy Transfer Between Tryptophan-214 in Human Serum Albumin and Acrylodan, Prodan, and Promen. J Protein Chem 23, 351–355 (2004). https://doi.org/10.1023/B:JOPC.0000032655.26249.ba

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:JOPC.0000032655.26249.ba

Search

Navigation