Abstract
The European tarnished plant bug, Lygus rugulipennis, is an important pest of agricultural and horticultural crops throughout Europe. Adult male L. rugulipennis were previously shown to be attracted to traps baited with live virgin females, which suggests the females produce a sex pheromone. Volatiles produced by virgin female L. rugulipennis were shown to contain three components, hexyl butyrate, (E)-2-hexenyl butyrate, and (E)-4-oxo-2-hexenal which elicited electroantennographic (EAG) responses from males in analyses by linked gas chromatography–electroantennography (GC-EAG). They were produced in 1.5:1:0.08 ratio, respectively, by single females. Collections from 1, 2, or 4 virgin females showed the proportions of hexyl butyrate and (E)-4-oxo-2-hexenal to increase relative to that of (E)-2-hexenyl butyrate with increasing number of females. Although these compounds were found in body extracts of both male and female L. rugulipennis, they were not detected in volatiles released by virgin males. EAG dose–response studies showed that both males and females responded to these chemicals with minimal differences in sensitivity between the sexes or to the three components, except that males were more responsive than females to (E)-4-oxo-2-hexenal at the two highest doses tested. Release rates of the compounds from rubber septa, polyethylene vials, and polyethylene sachets were measured under laboratory conditions. Four field tests were carried out using sticky traps baited with all possible binary and tertiary combinations of the three chemicals using different combinations of dispensing systems. Catches of male L. rugulipennis in baited traps were similar to those in unbaited traps. Significantly fewer females were caught on traps baited with blends containing hexyl butyrate than on traps without hexyl butyrate or unbaited traps in one test and overall. The roles of the three compounds and possible reasons for their failure to attract males are discussed.
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REFERENCES
Aldrich, J. R. 1988. Chemical ecology of the Heteroptera. Annu. Rev. Entomol. 33:211-238.
Aldrich, J. R., Numata, H., Borges, M., Bin, F., Waite, G. K., and Lusby, W. R. 1993. Artifacts and pheromone blends from Nezara spp. and other stink bugs (Heteroptera: Pentatomidae). Z. Naturforsch. 48c:73-79.
Blum, M. S. 1981. Chemical Defenses of Arthropods. Academic Press, New York.
Blum, M. S. 1996. Semiochemical parsimony in the arthropoda. Annu. Rev. Entomol. 41:353-374.
Blumenthal, M. A. 1978. The metathoracic gland system of Lygus lineolaris (Hetroptera: Miridae). MSc., Thesis, Cornell University, Ithaca New York, 142 pp.
Chinta, S., Dickens, J. S., and Aldrich, J. R. 1994. Olfactory reception of potential pheromones and plant odors by tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae). J. Chem. Ecol. 20:3251-3267.
Cork, A., Beevor, P. S., Gough, J. E., and Hall, D. R. 1990. Gas chromatography linked to electroantennography: A versatile technique for identifying insect semiochemicals, pp. 271-279, in A. R. McCaffery and I. D. Wilson (eds.). Chromatography and Isolation of Insect Hormones and Pheromones. Plenum, London.
Dickens, J. C. 1984. Olfaction in the boll weevil, Anthonomus grandis Boh. (Coleoptera: Curculionidae): Electroantennogram studies. J. Chem. Ecol. 10:1759-1785.
Drijfhout, E. P., Groot, A. T., Posthumus, M. A., Van Beek, T. A., and De Groot, A. 2002. Coupled gas chromatographic-electroantennographic responses of Lygocoris pabulinus (L.) to female and male produced volatiles. Chemoecology 12:113-118.
Erdelyi, C., Manninger, S., Manninger, K., Gergely, K., Hangyel, L., and Bernath, I. 1994. Climatic factors affecting population dynamics of the main seed pests of lucerne in Hungary. J. Appl. Entomol. 117:195-209.
Groot, A. T., Drijfhout, F. P., Heijboer, A., Van Beek, T. A., and Visser, J. H. 2001. Disruption of sexual communication in the mirid bug Lygocoris pabulinus by hexyl butyrate. Agric. For. Entomol. 3:49-55.
Gueldner, R. C. and Parrot, W. L. 1978. Volatile constituents of the tarnished plant bug. Insect Biochem. 8:389-391.
Hedin, P. A., Parrott, W. L., Tedders, W. L., and Reed, D. K. 1985. Field responses of the tarnished plant bug to its own volatile components. J. Miss. Acad. Sci. 30:63-66.
Ho, H. Y. and Millar, J. G. 2002. Identification, electroantennogram screening, and field bioassays of volatile chemicasl from Lygus hesperus Knight (Heteroptera: Miridae). Zool. Stud. 41:311-320.
Holopainen, J. 1989. Host plant preference of the tarnished plant bug Lygus rugulipennis Popp. (Het., Miridae). J. Appl. Entomol. 107:78-82.
Innocenzi, P. J., Hall, D. R., Sumathi, C., Cross, J. V., and Jacobson, R. J. 1998. Studies of the sex pheromone of the European tarnished plant bug, Lygus rugulipennis (Het. Miridae). Brighton Crop Prot. Conf.–Pests Dis. 8:829-832.
Kakazaki, M. and Sugie, H. 2001. Identification of female sex pheromone of the rice leaf bug, Trigonotylus caelestialium. J. Chem. Ecol. 27:2447-2458.
Marques, F. De A., McElfresh, J. S., and Millar, J. G. 2000. Female-produced sex pheromone of the predatory bug Geocoris punctipes. J. Chem. Ecol. 26:2843-2855.
McBrien, H. L. and Millar, J. G. 1999. Phytophagous bugs, pp. 277-304, in J. Hardie and A. K. Minks (eds.). Pheromones of Non-Lepidopteran Insects Associated with Agricultural Plants. CABI Publishing, Wallingford, UK.
Millar, J. G. and Rice, R. E. 1998. Sex pheromone of the plant bug Phytocoris californicus. J. Econ. Entomol. 23:1743-1755.
Millar, J. G., Rice, R.E., and Wang, Q. 1997. Sex pheromone of the mirid bug Phytocoris relativus. J. Chem. Ecol. 23:1743-1755.
Smith, R. F., Pierce, H. D., Jr., and Borden, J. H. 1991. Sex pheromone of the mullen bug, Campylomma verbasci (Meyer) (Heteroptera: Miridae). J. Chem. Ecol. 17:1437-1447.
Taksdal, G. and Sorum, O. 1971. Capsids (Heteroptera, Miridae) in strawberries, and their influence on fruit malformation. J. Hort. Sci. 46:43-50.
Todd, J. L. and Baker, T. C. 1999. Function of peripheral olfactory organs, pp. 67-96, in B. S. Hanson (ed.). Insect Olfaction. Springer, Berlin.
Torr, S. J., Hall, D. R., Phelps, R. J., and Vale, G. A. 1997. Methods for dispensing odour attractants for tsetse files (Diptera: Glossinidae). Bull. Entomol. Res. 87:299-311.
Ward, J. P. and Van Dorp, D. A. 1969. A stereospecific synthesis of 4-oxo-2-trans-hexenal Rec. Trav. Chim. 88:989-993.
Wardle, A. R., Borden, J. H., Pierce, H. D., Jr., and Gries, R. 2003. Volatile compounds released by disturbed and calm adults of the tarnished plant bug, Lygus lineolaris. J. Chem. Ecol. 29:931-944.
Zhang, Q. H. and Aldrich, J. R. 2003a. Male-produced anti-sex pheromone in a plant bug. Natur-wissenschaften 90:505-508.
Zhang, Q. H. and Aldrich, J. R. 2003b. Pheromones of milkweed bugs (Heteroptera: Lygaeidae) attract wayward plant bugs: Sex pheromones of two Phytocoris Mirids. J. Chem. Ecol. 29:1835-1851.
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Innocenzi, P.J., Hall, D.R., Cross, J.V. et al. Investigation of Long-Range Female Sex Pheromone of the European Tarnished Plant Bug, Lygus rugulipennis: Chemical, Electrophysiological, and Field Studies. J Chem Ecol 30, 1509–1529 (2004). https://doi.org/10.1023/B:JOEC.0000042065.19044.6d
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DOI: https://doi.org/10.1023/B:JOEC.0000042065.19044.6d