Abstract
The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.
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Kim, S.H., Kook, M.C. & Song, H.G. Optimal Conditions for the Retrieval of CD4 and CD8 Antigens in Formalin-Fixed, Paraffin-Embedded Tissues. Histochem J 35, 403–408 (2004). https://doi.org/10.1023/B:HIJO.0000039856.43632.66
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DOI: https://doi.org/10.1023/B:HIJO.0000039856.43632.66