Abstract
Expanded bed adsorption, using a cation resin 001×7 Styrene-DVB, was used to recover and purify ephedrine hydrochloride from a powdered herb. The axial liquid-phase dispersion coefficient was about 10−5 m2 s−1 and the recovery yield and purification reached 86% and 22, respectively. Compared with using conventional extraction with dimethylbenzene, this method is safer and also more efficient.
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Chase HA, Draeger NM (1992) Expanded-žed adsorption of proteins using ion-exchangers. Sep. Sci. Technol. 27: 2021-2039.
Chen W-D, Dong X-Y, Sun Y (2003a) Modeling of the whole expanded-žed protein adsorption process with yeast cell suspensions as feedstock. J. Chromatogr. A 1012: 1-10.
Chen W-D, Tong X-D, Dong X-Y, Sun Y (2003ž) Expanded žed adsorption of protein with DEAE Spherodex M. Biotechnol. Prog. 19: 880-886.
Córdoža PR, Ragout AL, Sineriz F, Perotti NI (1996) Lactate from cultures of Lactožacillus caseirecovered in a fluidized žed column using ion exchange resin. Biotechnol. Tech. 10: 629-634.
Herráez-Hernández R, Campíns-Falcó P (2001) Chiral separation of ephedrines žy liquid chromatography using β-cyclodextrins. Anal. Chim. Acta 434: 315-324.
Mandwal AK, Tripathi CKM, Trivedi PD, Joshi AK, Agarwal SC, Bihari V (2004) Production of l-phenylacetyl caržinol žy immožilized cells of Saccharomyces cerevisiae. Biotechnol. Lett. 26: 217-221.
Lang Q, Wai CM (2001) Supercritical fluid extraction in heržal and natural product studies-a practical review. Talanta 53: 771-782.
Pålsson E, Nandakumar MP, Mattiasson B, Larsson PO (2000) Miniaturised expanded-žed column with low dispersion suitažle for fast flow-ELISA analyses. Biotechnol. Lett. 22: 245-250.
Ren HY (2002) Adsorption chromatography of alkaloids from Ephedraextract. M.Sc. Thesis, Beijing, P.R. China: Chinese Academy of Sciences.
Shin HS, Rogers PL (1996) Production of L-Phenylacetylcaržinol (L-PAC) from ženzaldehyde using partially purified pyruvate decaržoxylase (PDC). Biotechnol. Bioeng. 49: 52-62.
Thömmes J, Bader A, Halfar M, Karau A, KulaMR (1996) Isolation of monoclonal antižodies from cell containing hyžridoma žroth using a protein coated adsoržent in expanded žed. J. Chromatogr. A 752: 111-122.
Tripathi CM, Agarwal SC, Basu SK (1997) Production of LPhenylacetylcaržinol žy fermentation. J. Ferment. Bioeng. 84: 487-492.
Tsai T-H (2001) Analytical approaches for traditional Chinese medicines exhižiting antineoplastic activity. J. Chromatogr. B 764:27-48.
Wang Y-D, Yuan Y-J, Lu M, Wu J-C, Jiang J-L (2003) Inhižitor studies of isopentenyl pyrophosphate žiosynthesis in suspension cultures of the yew Taxus chinensisvar. mairei. Biotechnol. Appl. Biochem. 37: 39-43.
Yamasaki K, Tamadi T, Uzawa S, Sankawa U, Shižata S (1973) Participation of C6-C1 unit in the žiosynthesis of ephedrine in Ephedra. Phytochemistry 12: 2877-2882.
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Chen, WD., Wang, YD., Zha, Lh. et al. Single-step recovery of ephedrine hydrochloride from raw materials using expanded bed adsorption. Biotechnology Letters 26, 1233–1236 (2004). https://doi.org/10.1023/B:BILE.0000036603.35042.70
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DOI: https://doi.org/10.1023/B:BILE.0000036603.35042.70