Abstract
Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 °C. The highest total lipase/esterase activity (57 U l−1) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l−1, respectively), and finally by Thermus sp. (16 U l−1). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l−1). All enzymes were stable at 80 °C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate).
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Domínguez, A., Sanromán, A., Fuciños, P. et al. Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.. Biotechnology Letters 26, 705–708 (2004). https://doi.org/10.1023/B:BILE.0000024092.27943.75
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DOI: https://doi.org/10.1023/B:BILE.0000024092.27943.75