Abstract
Soils at the contaminated Aljustrel mining area situated in SWPortugal in the Iberian Pyrite Belt, and Cistusladanifer L., an autochthonal aromatic bush, were investigated to quantify the most relevant elements present in soils and in the plant, and to evaluate the possibility of phytoremediation of that area, particularly concerning the elements of higher pollutant potential.Multielemental (Mg, S, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn, As, Br and Pb) characterisation of the soils and C. ladanifer leaves was carried out by Energy-Dispersive X-Ray Fluorescence spectrometry (EDXRF). Subsequently, due to their abundance in the soil and significant elevation compared to the control site, potential toxicity and/or biological significance, Mn, Cu, Zn, and Pb were determined by Atomic Absorption Spectrometry (AAS) in samples of soils, as well as in the roots and leaves of plants. C. ladanifer is able to survive and grow in soils having high concentrations of such toxic elements and to accumulate Mn.The bioavailability of Mn, Cu, Zn and Pb in the soils was evaluated by determining the contents of the elements in a solution of 0.5 M ammonium acetate, 0.5 M acetic acid and 0.02 M EDTA, pH 4.7, after soil extraction. Comparison of the values obtained with the corresponding element concentration in leaves give us a model of C. ladanifer's capacity to avoid these metals to reach toxic levels in the plant or their toxic effects when such levels are exceed, as in the case of Mn.
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References
Alados, C. L., Navarro, T. and Cabezudo, B.: 1999, 'Tolerance assessment of Cistus ladanifer to serpentine soils by developmental stability analysis', Plant Ecology 143, 51–66.
Alvarenga, P., AraÚjo, M. F. and Silva, J. A.: 1998, 'Phytoremediation: A Study of Metals in the Soil-Plant System in Aljustrel's Mining Area Regarding Cystus ladanifer L', Proceeding of 1st International Meeting of Aromatic and Medical Mediterranean Plants, Ansião, Portugal, 24–26 April 1998, pp. 51–56.
AraÚjo, M. F. D., Valério P. and Jouanneau, J.-M.: 1998, 'Heavy metal assessment in Sediments of the Ave River Basin (Portugal) by EDXRF', X-Ray Spectrometry 27, 305–312.
AraÚjo, M. F. D., Cruz, A., Humanes, M., Lopes, M. T., Silva, J. A. L. and FraÚsto da Silva, J. J. R.: 1999, 'Elemental composition of Demospongiae from the eastern Atlantic coastal waters', Chem. Spec. Bioavail. 11, 25–36.
Baker, A. J. M.: 1981, 'Accumulators and excluders – Strategies in the response of plants to heavy metals', J. Plant Nutrition 3, 643–654.
Bolaños, M. M. and Lopez E. G.: 1949, Jarales y Jaras, Ed. Ares, Madrid.
De Koe, T., Geldmeyer, K., Jaques, N. M. M.: 1992, 'Measuring maximum root growth instead of longest root elongation in metal tolerance tests for grasses (Agrostis capillaris, Agrostis delicatula and Agrostis castellana)', Plant and Soil 144, 305–308.
De Koe, T.: 1994, 'Agrostis castellana and Agrostis delicatula on heavy metals and arsenic enriched sites in NE Portugal', The Sci. of the Total Environ. 145, 103–109.
Delgado, J. A., Serrano, J. M., Lopez, F. and Acosta, F.J.: 2001, 'Heat shock, mass-dependent germination, and seed yield as related components of fitness in Cistus ladanifer', Environ. Exp. Bot. 46, 11–20.
Dewis, J. and Freitas, F.: 1984, 'Physical and chemical methods of soil and water analysis', FAO Soils Bulletin, 10.
Dias, L. S. and Dias, A. S.: 1987, 'Cistus ladanifer L. (Esteva). Importância, Gestão e Ecologia' Actas do 2 ? Congresso Sobre o Alentejo, 1987, pp. 243–250.
Esser, K. B.: 1995, 'Reference concentrations for heavy metals in mineral soils, oat and orchard grass (Dáctylis glomeráta) from three agricultural regions in Norway', Water, Air, and Soil Pollut. 89, 375–397.
Farago, M. E. and Merha, A.: 1991, 'Uptake of Elements by the Copper-Tolerant Plant Armeria maritime', in E. Merian (ed.), Metal Compounds in Environment and Life – Interrelation Between Chemistry and Biology Proceedings of the Fourth Hans Wolfgang Nürnberg Memorial Workshop, 1991, 4, pp. 163–169.
Farago, M. E., Cole, M., Xiao, X. and Vaz, M. C.: 1992, 'Preliminary assessment of metal bioavailability to plants in the Neves Corvo Area of Portugal', Chem. Spec. and Bioavail. 4, 19–27.
FraÚsto da Silva, J. J. R. and Williams, R. J. P.: 2001, The Biological Chemistry of the Elements – The Inorganic Chemistry of Life, 2nd ed., Clarendon Press, Oxford.
Gordillo, M. C. T., Gi, E. P., Gonzalez, M. A. R., Murciego, A. M. and Ostapczuk, P.: 2001, 'Potentiometric stripping analysis (PSA) for monitoring of antimony in samples of vegetation from a mining area', Fres. J. Analyt. Chem. 370, 434–437.
Hadfield, P.: 1998, 'Greening the desert', New Scientist 2143, 21.
Henriques, F. S. and Fernandes, J. C.: 1991, 'Metal uptake and distribution in rush (Juncus conglomeratus L.) plants growing in pyrites mine tailings at Lousal, Portugal', The Sci. of the Total Environ. 102, 253–260.
Jung, M. C. and Thornton, I.: 1996 'Heavy metal contamination of soils and plants in the vicinity of a lead-zinc mine, Korea', Appl. Geochem. 11, 53–59.
Kennedy, V. H., Sanchez, A. L., Oughton, D. H. and Rowland, A. P.: 1997, 'Use of single and sequential chemical extractants to assess radionuclide and heavy metal availability from soils for root uptake – Critical review', Analyst 122, 89R–100R, and references therein.
Nunez Oliveira, E. and Martinez Abaigar, J. and Escudero, J. C.: 1994, 'Chlorophyll content of a Mediterranean Shrub (Cistus Ladanifer, L.) over a latitude and altitude gradient in the Iberian Peninsula', Photosynthetica 30, 133–142.
Rauser, W. E.: 1984, 'Partial purification and characterization of copper-binding protein from roots of Agrostis gigantean', Roth. J. Plant Physiol. 115, 148–152.
Salomons, W. and Förstner, U.: 1984, Metals in the Hydrocycle, Springer-Verlag, Berlin.
Salt, D. E., Blaylock, M., Kumar, N. P. B. A, Dushenkov, V., Ensley, B. D., Chet, I. and Raskin, I.: 1995, 'Phytoremediation: A novel strategy for the removal of toxic metals from the environment using plants', Bio/Technology 13, 468–474.
Sanchez, J., Vaquero, M. C. and Legorburu, I.: 1994 'Metal pollution from old lead-zinc mine works: Biota and sediment from Oiartzun Valle', Environ. Technol. 15, 1069–1076.
Schermerhorn, L. J. G., Zbyszewski, G. and Veiga Ferreira, O.: 1987, Notícia Explicativa da Folha 42-D Aljustrel, Serviços Geológicos de Portugal, Lisboa.
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Alvarenga, P.M., Araújo, M.F. & Silva, J.A.L. Elemental Uptake and Root-Leaves Transfer in Cistus Ladanifer L. Growing in a Contaminated Pyrite Mining Area (Aljustrel-Portugal). Water, Air, & Soil Pollution 152, 81–96 (2004). https://doi.org/10.1023/B:WATE.0000015333.24165.5e
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DOI: https://doi.org/10.1023/B:WATE.0000015333.24165.5e