Abstract
A molecular analysis of complement components (C3, C4, and factor B) in human saliva was performed by SDS-PAGE and immunoblotting. Complement C3 was detected as a molecule composed of a 115-kDa α-chain linked to a 70-kDa β chain by disulfide bonds, and C3 levels ranged from 0.52 to 15.0 /μg/ml (n = 15). C4 was detected as a triple-chain molecule (98-kDa α chain, 73-kDa β chain, and 33-kDa γ chain) linked by disulfide bonds, and C4 levels ranged from 0.086 to 4.8 μg/ml. Factor B was detected as a 100-kDa single chain, and factor B levels ranged from 0.042 to 0.62/μg/ml. The sizes and subunit structures of the complement components in human saliva were compatible with those reported in human serum. The results of a hemolytic assay indicated that the complement molecules in human saliva were functionally active. These complement components may participate in the local immune and inflammatory responses in the oral cavity.
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Andoh, A., Fujiyama, Y., Kimura, T. et al. Molecular Characterization of Complement Components (C3, C4, and Factor B) in Human Saliva. J Clin Immunol 17, 404–407 (1997). https://doi.org/10.1023/A:1027320425291
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DOI: https://doi.org/10.1023/A:1027320425291