Abstract
Human α2(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l−1 of E. coli culture), a two-site immunometric assay based on two monoclonal antibodies with different epitopes was developed.
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Frang, H., Hellman, J., Goldman, A. et al. A two-site immunometric assay for the detection of the expression level of α2(C2)-adrenergic receptor produced in Escherichia coli. Biotechnology Letters 22, 1963–1966 (2000). https://doi.org/10.1023/A:1026766330471
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DOI: https://doi.org/10.1023/A:1026766330471