Abstract
The methods of studying the genetic diversity of wild animals using tissue samples collected without harming the studied populations are discussed for the red deer (Cervus elaphus) fur samples. The methods are proposed for the collection and storage of fur samples in field conditions. The optimal methods of DNA isolation from the fur and other tissues (dry, alcohol-fixed, and frozen skin and muscle) of the red deer and other ungulates were selected, and the conditions of PCR for amplification of the mitochondrial DNA fragments were determined. The comparison between the pure-blood red deers from Central England and the Voronezh State Reserve (Russia) was performed using a cytochrome b fragment of the mitochondrial DNA.
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REFERENCES
Avise, J.C., Molecular Markers, Natural History and Evolution, New York: Chapman and Hall, 1994.
Caughley, G. and Gunn, A., Conservation Biology in Theory and Practice, Oxford: Blackwell Science, 1996.
Higgins, D.G. and Sharp, P.M., CLUSTAL: A Package for Performing Multiple Sequence Alignment on a Microcomputer, Gene, 1988, vol. 73, no. 1, pp. 237–244.
Hillis, D.M., Mable, B.K., Larson, A., et al., Molecular Systematics, Nucleic Acids IV: Sequencing and Cloning, Sinauer Associates, 1996, pp. 321–354.
Hoss, M. and Paabo, S., Mammoth DNA Sequences, Nature, 1994, vol. 370, p. 333.
Hoss, M.A., Kohn, M., Paabo, S., et al., Excrement Analysis by PCR, Nature, 1992, vol. 359, p. 199.
Kocher, T.D., Thomas, W.K., Meyer, A., et al., Dynamics of Mitochondrial DNA Evolution in Animals: Amplification and Sequencing with Conserved Primers, Proc.Nat.Acad.Sci.USA, 1989, vol. 86, pp. 6196–6200.
Kohn, M.H. and Wayne, R.K., Facts from Faeces Revisited, Tree, 1997, vol. 12, no. 6, pp. 223–227.
Kohn, M., Knaucr, F., Stofella, A., et al., Conservation Genetics of the European Brown Bear-A Study Using Excremental PCR of Nuclear and Mitochondrial Sequences, Mol.Ecol., 1995, vol. 4, pp. 95–103.
Martin, A.P., Naylor, G.J.P., and Palumbi, S.R., Rates of Mitochondrial DNA Evolution in Sharks Are Slow Compared to Mammals, Nature, 1992, vol. 357, pp. 153–155.
Morin, P.A., Wallis, J., Moore, J.J., and Woodruff, D.S., Paternity Exclusion in a Community of Wild Chimpanzees Using Hypervariable Simple Sequence Repeats, MoI.Ecol., 1994, vol. 3, pp. 469–478.
Morin, P.A. and Woodruff, D.S., Molecular Genetic Approaches in Conservation, Noninvasive Genotyping for Vertebrate Conservation, Oxford: Univ. Press, 1996, pp. 298–313.
Palumbi, S.R., Molecular Systematics, Nucleic Acids II: The Polymerase Chain Reaction, Sinauer Associates, 1996, pp. 205–247.
Sanderson, M.J., Baldwin, B.G., Bharathan, G., et al., The Growth of Phylogenetic Information and the Need for a Phylogenetic Data Base, Syst.Biol., 1993, vol. 42, pp. 562–568.
Silver, J., PCR: A Practical Approach, Inverse Polymerase Chain Reaction, Oxford: Univ. Press, 1992, pp. 137–146.
Soule, M., Conservation Biology: An EvolutionaryEcological Perspective, Thresholds for Survival: Maintaining Fitness and Evolutionary Potential, Sunderland, MA: Sinauer, 1980, pp. 111–124.
Taberlet, P., Bear Conservation Genetics, Nature, 1992, vol. 358, p. 197.
Taberlet, P. and Bouvet, J., Mitochondrial DNA Polymorphism, Phylogeography, and Conservation Genetics of the Brown Bear (Ursus arctos) in Europe, Proc.Roy.Soc.Lond.(ser.B), 1994, vol. 255, pp. 195–200.
Taberlet, P., Griffin, S., Goossens, B., et al., Reliable Genotyping of Samples with Very Low DNA Quantities Using PCR, Nucl.Acids Res., 1996, vol. 24, no. 16, pp. 3189–3194.
Yalden, D.W., The Past, Present and Future of Deer in Britain, Population Ecology, Management and Welfare of Deer.3rd Symposium.9–10 April 1997. Book of Abstracts, Manchester Metropolitan University, 1997, p. 5.
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Kholodova, M.V., Easton, E. & Milner-Gulland, E.J. The Use of Fur Collected in the Field for Studying the Genetic Diversity of Wild Ungulates. Biology Bulletin 27, 585–590 (2000). https://doi.org/10.1023/A:1026611615608
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DOI: https://doi.org/10.1023/A:1026611615608