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Aptamer DNA: A New Type of Thrombin Inhibitors

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Abstract

The formation of complexes between various thrombin preparations and 30-mer aptamer DNA was comparatively studied, and a correlation between the complex formation and the fibrinogen-hydrolyzing activity of thrombin was found. The aptamer DNA was shown to inhibit the formation of fibrin from fibrinogen.

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References

  1. Gold, L., Polisky, B., Uhlenbeck, O., and Yarus, M., Annu. Rev. Biochem., 1995, vol. 64, pp. 763–797.

    Google Scholar 

  2. Gold, L., J. Biol. Chem., 1995, vol. 270, pp. 13 581–13 584.

    Google Scholar 

  3. Osborne, S.E., Matsumura, I., and Ellington, A.D., Curr. Opin. Chem. Biol., 1997, vol. 1, pp. 5–9.

    Google Scholar 

  4. Kopylov, A.M., Spiridonova, V.A., and Park, K.-Kh., Ross. Khim. Zh., 1998, vol. 42, pp. 89–96.

    Google Scholar 

  5. Kopylov, A.M. and Spiridonova, V.A., Mol. Biol. (Moscow), 2000, vol. 34, pp. 1097–1113.

    Google Scholar 

  6. Tuerk, C. and Gold, L., Science, 1990, vol. 249, pp. 505–510.

    Google Scholar 

  7. Takeno, H., Yamamoto, S., Tanaka, T., Sakano, Y., and Kikuchi, Y., J. Biochem. (Tokyo), 1999, vol. 125, pp. 1115–1119.

    Google Scholar 

  8. Takeno, H., Tanaka, T., and Kikuchi, Y., Nucl. Acids Symp. Ser., 1997, vol. 37, pp. 249–250.

    Google Scholar 

  9. Fukuda, K., Vishnuvardhan, D., Sekiya, S., Hwang, J., Kakiuchi, N., Taira, K., Shimotohno, K., Kumar, P.K., and Nishikawa, S., Eur. J. Biochem., 2000, vol. 267, pp. 3685–3694.

    Google Scholar 

  10. Bless, N.M., Smith, D., Charlton, J., Czermak, B.J., Schmal, H., Friedl, H.P., and Ward, P.A., Curr. Biol., 1997, vol. 7, pp. 877–880.

    Google Scholar 

  11. Charlton, J., Sennello, J., and Smith, D., Chem. Biol., 1997, vol. 4, pp. 809–816.

    Google Scholar 

  12. Charlton, J., Kirschenheuter, G.P., and Smith, D., Biochemistry, 1999, vol. 36, pp. 3018–3026.

    Google Scholar 

  13. Panchenko, E.P. and Dobrovol'skii, A.B., Trombozy v kardiologii. Mekhanizmy razvitiya i vozmozhnosti terapii (Thromboses in Cardiology: Mechanisms of Development and Possibilities of Therapy), Moscow: Sport i Kul'tura, 1999, pp. 55–74.

    Google Scholar 

  14. Machovich, R., The Thrombin, Boca Raton: CRP Inc., 1984, vol. 1, pp. 131–160.

    Google Scholar 

  15. Bock, L.C., Griffin, L.C., Latham, J.A., Vermaas, E.H., and Toole, J.J., Nature, 1992, vol. 355, pp. 564–566.

    Google Scholar 

  16. Macaya, R.F., Waldron, J.A., Beutel, B.A., Gao, H., Joesten, M.E., Yang, M.M., Patel, R., Bertelsen, A.H., and Cook, A.F., Biochemistry, 1995, vol. 34, pp. 4478–4492.

    Google Scholar 

  17. Tsiang, M., Gibbs, C.S., Griffin, L.C., Dunn, K.E., and Leung, L.L., J. Biol. Chem., 1995, vol. 270, pp. 19370–19376.

    Google Scholar 

  18. Tasset, D.M., Kubik, M.F., and Steiner, W., J. Mol. Biol., 1997, vol. 272, pp. 688–698.

    Google Scholar 

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Authors and Affiliations

  1. Belozersky Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    V. A. Spiridonova

  2. Faculty of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    E. V. Rog & A. M. Kopylov

  3. Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    T. N. Dugina & S. M. Strukova

Authors
  1. V. A. Spiridonova
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  2. E. V. Rog
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  3. T. N. Dugina
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  4. S. M. Strukova
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  5. A. M. Kopylov
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Spiridonova, V.A., Rog, E.V., Dugina, T.N. et al. Aptamer DNA: A New Type of Thrombin Inhibitors. Russian Journal of Bioorganic Chemistry 29, 450–453 (2003). https://doi.org/10.1023/A:1026001609421

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  • DOI: https://doi.org/10.1023/A:1026001609421

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