Photosynthetica 2003, 41(1):91-96 | DOI: 10.1023/A:1025816614572

Comparison of Aerial and Submerged Leaves in Two Amphibious Species, Myosotis scorpioides and Ranunculus trichophyllus

M. Germ1, A. Gaber¹èik1,2
1 National Institute of Biology, Ljubljana, Slovenia
2 Department of Biology, Biotechnical Faculty, Ljubljana, Slovenia

Both amphibious species, Myosotis scorpioides and Ranunculus trichophyllus, thrive in a stressful environment (alternated flooding and drying), which is variable regarding water and radiation regimes. Plants from the field and plants grown under controlled water table maintained at 40 cm were analysed for content of chlorophyll (Chl) and UV-B screening compounds, and the efficiencies of PS2 and electron transport systems. We detected no significant differences in contents of Chl and UV-B screening compounds between submerged and aerial leaves. The measurements of respiratory potential and photochemical efficiency revealed the presence of permanent stress in M. sporpioides in the natural environment. Differences in physiological responses of submerged and aerial leaves indicated that the terrestrial environment was more favourable for M. scorpioides than for R. trichophyllus. Characteristics of both species suggested that R. trichophyllus might be a phylogenetically older aquatic plant than M. scorpioides.

Additional key words: chlorophylls; electron transport system activity; fluctuating water level; fluorescence induction; leaf length; photochemical efficiency of photosystem 2; stomata number; UV-B screening compounds

Published: March 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Germ, M., & Gaber¹èik, A. (2003). Comparison of Aerial and Submerged Leaves in Two Amphibious Species, Myosotis scorpioides and Ranunculus trichophyllus. Photosynthetica, 41(1), 91-96. doi: 10.1023/A:1025816614572
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adamec, L.: Interrelationship between uptake of mineral ions and photosynthesis in submerged aquatic plants.-Biol. Plant. 34: 501, 1992.
    2. Adams, W.W., III, Demmig-Adams, B., Winter, K., Schreiber, U.: The ratio of variable to maximum chlorophyll fluorescence from photosystem II, measured in leaves at ambient temperature and at 77 K, as an indicator of the photon yield of photosynthesis.-Planta 180: 166-174, 1990. Go to original source...
    3. Albrecht, G., Biemelt, S.: A comparative study on carbohydrate reserve and ethanolic fermentation in the roots of two wetland and non-wetland species after commencement of hypoxia.-Physiol. Plant. 104: 81-86, 1998. Go to original source...
    4. Albrecht, G., Biemelt, S., Baumgartner, S.: Accumulation of fructans following oxygen deficiency stress in related plant species with different flooding tolerances.-New Phytol. 136: 137-144. 1998. Go to original source...
    5. Amthor, J.S.: Higher plant respiration and its relationship to photosynthesis.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 71-101. Springer-Verlag, Berlin-Heidelberg-New York 1995. Go to original source...
    6. Barrat-Segretain, M.H., Amoros, C.: Recovery of riverine vegetation after experimental disturbance; a field test of the patch dynamic concept.-Hydrobiologia 321: 53-68, 1996. Go to original source...
    7. Björn, L.O.: Effects of ozone depletion and increased ultraviolet-B radiation on terrestrial plants.-In: Baumstark-Khan, C. (ed.): Fundamentals for the Assessment of Risks from Environmental Radiation. Pp. 463-470. Kluwer Academic Publishers, Dordrecht 1999. Go to original source...
    8. Blumthaler, M., Ambach, W., Rehwald, W.: Solar UV-A and UV-B radiation fluxes at two alpine stations at different altitudes.-Theor. appl. Climatol. 46: 39-44, 1992. Go to original source...
    9. Bucher, M., Brandle, R., Kuhlemeier, C.: Glycolytic gene expression in amphibious Acorus calamus L. under natural conditions.-Plant Soil 178: 75-82 1996. Go to original source...
    10. Caldwell, M.M.: Solar ultraviolet radiation as an ecological factor for alpine plants.-Ecol. Monogr. 38: 243-268, 1968. Go to original source...
    11. Clevering, O.A., Blom, C.W.P.M., Van Vierssen, W.: Growth and morphology of Scirpus lacustris and S. maritimus seedlings as affected by water level and light availability.-Funct. Ecol. 10: 286-296, 1996. Go to original source...
    12. Critchley, C.: Photoinhibition.-In: Raghevendra, A.S. (ed.): Photosynthesis. A Comprehensive Treatise. Pp. 264-272. Cambridge University Press, Cambridge 1998.
    13. Dahlgren, G., Cronberg, N.: Species differentiation and relationship in Ranunculus subgenus Batrachium (Ranunculaceae) elucidated by isozyme electrophoresis.-Symb. Bot. Ups. 31(3): 91-104, 1996.
    14. Dahlgren, G., Svensson, L.: Variation in leaves and petals of Ranunculus subgenus Batrachium on the Aegean Islands, analysed by multivariate analysis.-Bot. J. Linn. Soc. 14: 253-270, 1994. Go to original source...
    15. Dalla Vecchia, G., Cuccato, F., La Rocca, N., Larcher, W., Rascio, N.: Endodermis-like sheats in the submerged freshwater macrophyte Ranunculus trichophyllus Chaix.-Ann. Bot. 83: 93-97, 1999. Go to original source...
    16. Fiasson, J.L., Gluchoff-Fiasson, K., Dahlgren, G.: Flavonoid patterns in European Ranunculus L. subgenus Batrachium (Ranunculaceae).-Biochem. Syst. Ecol. 25: 327-333, 1997. Go to original source...
    17. Frost-Christensen, H., Sand-Jensen, K.: The quantum efficiency of photosynthesis in macroalgae and submerged angiosperms.-Oecologia 91: 377-384, 1992. Go to original source...
    18. Germ, M., Mazej, Z., Gaber¹èik, A., Häder, D.P.: The influence of enhanced UV-B radiation on Batrachium trichophyllum and Potamogeton alpinus - aquatic macrophytes with amphibious character.-J. Photochem. Photobiol. B 66: 37-46, 2002. Go to original source...
    19. Gluchoff-Fiasson, K., Fiasson, J.L., Waton, H.: Quercetin glycosides from European aquatic Ranunculus species of subgenus Batrachium.-Phytochemistry 45: 1063-1067, 1997. Go to original source...
    20. Hennion, F., Fiasson, J.L., Gluchoff-Fiasson, K.: Morphological and phytochemical relationships between Ranunculus species from Hes Kerguelen.-Biochem. Syst. Ecol. 22: 533-542, 1994. Go to original source...
    21. Jeffrey, S.W., Humphrey, G.F.: New spectrophometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton.-Biochem. Physiol. Pflanzen 167: 191-194, 1975. Go to original source...
    22. Kenner, R.A., Ahmed, S.I.: Measurements of electron transport activities in marine phytoplankton.-Mar. Biol. 33: 119-127, 1975. Go to original source...
    23. Les, D.H., Sheridan, D.J.: Biochemical heterophylly and flavonoid evolution in North American potamogeton (Potamogetonaceae).-Amer. J. Bot. 4: 453-465, 1990. Go to original source...
    24. Madsen, T.V., Breinholt, M.: Effect of air contact on growth, inorganic carbon sources, and nitrogen uptake by an amphibious freshwater macrophyte.-Plant Physiol. 107: 149-154, 1995. Go to original source...
    25. Martinèiè, A., Wraber, T., Jogan, N., Ravnik, V., Podobnik, A., Turk, B., Vre¹, B.: [Flora of Slovenia.]-Tehni¹ka zalo¾ba Slovenije, Ljubljana 1999. [In Slovenian.]
    26. Mazej, Z., Gaber¹èik, A.: ETS-activity as a measure of vitality of different macrophyte species.-Phyton 39: 181-185, 1999.
    27. Menadue, Y., Crowden, R.K.: Leaf polymorphism in Ranunculus nanus Hook. (Ranunculaceae).-New Phytol. 114: 265-274, 1990. Go to original source...
    28. Nielsen, S.L.: A comparison of aerial and submerged photosynthesis in some Danish amphibious plants.-Aquat. Bot. 45: 27-40, 1993. Go to original source...
    29. Nielsen, S.L., Sand-Jensen, K.: Photosynthetic implications of heterophylly in Batrachium peltatum (Schrank) Presl.-Aquat. Bot. 44: 361-371, 1993. Go to original source...
    30. Nielsen, S.L., Sand-Jensen, K.: Growth rates and morphological adaptations of aquatic and terrestrial forms of amphibious Littorella uniflora (L.). Aschers.-Plant Ecol. 129: 135-140, 1997. Go to original source...
    31. Osmond, C.B., Valaane, N., Haslam, S.M., Votila, P., Roksandic, Z.: Comparison of δ13 values in leaves of aquatic macrophytes from different habitats in Britain and Finland; some implications for photosynthetic processes in aquatic plants.-Oecologia 50: 117-124, 1981. Go to original source...
    32. Packard, T.T.: The measurement of respiratory electron transport activity in marine phytoplankton.-J. mar. Res. 29: 235-243, 1971.
    33. Pedersen, O., Sand-Jensen, K.: Adaptation of submerged Lobelia dortmanna to aerial life form: morphology, carbon sources and oxygen dynamics.-Oikos 65: 89-96, 1992. Go to original source...
    34. Rascio, N., Cuccato, F., Dalla Vecchia, F., La Rocca, N., Larcher, W.: Structural and functional features of the leaves of Ranunculus trichophyllus Chaix., a freshwater submerged macrophyte.-Plant Cell Environ. 22: 205-212, 1999. Go to original source...
    35. Sand-Jensen, K., Pedersen, M.F., Nielsen, S.L.: Photosynthetic use of inorganic carbon among primary and secondary water plants in streams.-Freshwater Biol. 27: 283-293, 1992. Go to original source...
    36. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-61. Springer-Verlag, Berlin-Heidelberg-New York 1995. Go to original source...
    37. Sullivan, J.H., Teramura, A.H.: The effects of UV-B radiation on loblolly pine. 2. Growth of field-grown seedlings.-Trees Struct. Funct. 6: 115-120, 1992. Go to original source...
    38. Tosserams, M., Rozema, J.: Effects of ultraviolet-B radiation (UV-B) on growth and physiology of the dune grassland species Calamagrostis epigeios.-Environ. Pollut. 89: 209-214, 1996. Go to original source...
    39. Tratnik, I.: [Genus Ranunculus subg. batrachium in Slovenia.]-Graduation Thesis. Dep. Biology, Biotechnical Faculty, Ljubljana 1977. [In Slovenian.]
    40. Ueno, O.: Structural characterization of photosynthetic cells in an amphibious sedge, Eleocharis vivipara, in relation to C3 and C4 metabolism.-Planta 199: 382-393, 1996. Go to original source...
    41. Vadstrup, M., Madsen, T.V.: Growth limitation of submerged aquatic macrophytes by inorganic carbon.-Freshwater Biol. 34: 411-419, 1996. Go to original source...
    42. Webster, S.D.: A chromatographic investigation of the flavonoids of Ranunculus L. subgenus Batrachium (DC.) A. Gray (water buttercups) and selected species in subgenus Ranunculus.-Aquat. Bot. 40: 11-26, 1991. Go to original source...

    Return to the content