Skip to main content
SpringerLink
Log in

Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Mg2+ at 3.5 mM increased the tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae to ethanol. After 9 h of exposure to 20% (v/v) ethanol at 30 °C, all cells died whereas over 50% remained viable for the cells grown with Mg2+. The effect of Mg2+ is closely related to its ability to decrease plasma membrane permeability of cells subjected to ethanol stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bai FW, Jin Y, Feng PS, He XL (1999) Studies of ethanol fermentation using a fusant SPSC flocs – description of flocs, growth and ethanol fermentation kinetics. Chin. J. Biotechnol. 15: 455–460.

    Google Scholar 

  • Casey GP, Ingledew M (1986) Ethanol tolerance in yeasts. Crit. Rev. Microbiol. 13: 219–290.

    Google Scholar 

  • D'Amore T, Stewart GG (1987) Ethanol tolerance of yeast. Enzyme Microb. Technol. 9: 322–330.

    Google Scholar 

  • Hayashida S, Feng DD, Hongo M (1975) Physiological properties of yeast cells grown in the proteolipid supplemented media. Agric. Biol. Chem. 39: 1025–1031.

    Google Scholar 

  • Hayashida S, Feng DD, Ohta K, Chaitiumvong S, Hongo M (1976) Compositions and a role of Aspergillus oryzae proteolipid as a high concentration alcohol-producing factor. Agric. Biol. Chem. 40: 73–78.

    Google Scholar 

  • Ingram LO, Buttke TM (1984) Effects of alcohols on microorganisms. Adv. Microb. Physiol. 25: 253–300.

    Google Scholar 

  • Mizoguchi H, Hara S (1996) Effect of fatty acid saturation in membrane lipid bilayers on simple diffusion in the presence of ethanol at high concentrations. J. Ferment. Bioeng. 81: 406–411.

    Google Scholar 

  • Rosa MF, Sá-Correia I, Novais JM (1987) Production of ethanol at high temperatures in the fermentation of Jerusalem artichoke juice and a simple medium by Kluyveromyces marxianus. Biotechnol. Lett. 9: 441–444.

    Google Scholar 

  • Rosa MF, Sá-Correia I, Novais JM (1988) Improvements in the ethanol tolerance of Kluyveromyces fragilis in Jerusalem artichoke juice. Biotechnol. Bioeng. 31: 705–710.

    Google Scholar 

  • Thomas DS, Hossack JA, Rose AH (1978) Plasma membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Arch. Microbiol. 17: 239–245.

    Google Scholar 

  • Yang JY, Park KH, Pek UH, Yu JH (1990) Screening and characterization of the high alcohol producing Saccharomyces cerevisiae D 1. Korean J. Appl. Microbiol. Biotechnol. 18: 511–516.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Engineering, Dalian University of Technology, 116024, Dalian, P.R. China

    Chun-Keng Hu, Feng-Wu Bai & Li-Jia An

  2. Department of Bioengineering, National Huaqiao University, 362011, Quanzhou, Fujian, P.R. China

    Chun-Keng Hu

Authors
  1. Chun-Keng Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng-Wu Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li-Jia An
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li-Jia An.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, CK., Bai, FW. & An, LJ. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters 25, 1191–1194 (2003). https://doi.org/10.1023/A:1024583503274

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024583503274

Search

Navigation