Abstract
Fiji disease fijivirus (FDV) genomic segments 1 (S1) and 3 (S3) were completely sequenced. FDV S1 comprised 4532 nt and was predicted to encode a 170.6 kDa protein. FDV S3 comprised 3623 nt and was predicted to encode a 135.5 kDa protein. The terminal sequences of S1 and S3 were 5′ AAGUUUUU......CAGCUAGCGUC 3′ and 5′ AAGUUUUU......CAGCAGAUGUC 3′, respectively, and located immediately adjacent to these sequences were 12 bp imperfect inverted repeats. The predicted translation product of FDV S1 showed highest similarity to Rice black-streaked dwarf virus (RBSDV) S1 and is thought to encode the viral RNA-dependent RNA polymerase (RdRp). The predicted translation product of FDV S3 was found to be most similar to RBSDV S4 which is thought to encode the 'B-spike' protein. The FDV sequence contained an ATP/GTP binding motif and a leucine zipper motif, but these motifs were not found in the RBSDV sequence. Phylogenetic analysis based on the amino acid sequences of the RdRp of FDV S1 and other reoviruses revealed that the fijiviruses form a cluster close to the oryzaviruses. The RdRp sequences were grouped into genera that were consistent with the current reovirus classification scheme that is based on physico-chemical and biological properties.
Similar content being viewed by others
References
Hughes C. and Robinson P., in Martin J.P., Abbott E.V., and Hughes C.G. (eds.), Sugar Cane Diseases of the World, Vol. 1. Elsevier, Amsterdam, 1961, pp. 389-405.
Smith G.R., in Rott P., Bailey R.A., Comstock Croft B.J., and Saumtally A.S. (eds.), A Guide to Sugarcane Diseases, Cirad Publications Service, Montpellier, 2000, pp. 239-244.
Australian Department of Foreign Affairs and Trade. [http://www.dfat.gov.au/facts/affaoverview.html].
Nuss D.L., and Dall D.J., Adv Virus Res 38, 249-306, 1990.
Mertens P., Arella M., Attoui H., Belloncik S., Bergoin M., Boccardo G., Booth T., Chiu W., Diprose J., and Duncan R., in van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeogh D.J., Pringle C.R., and Wickner R.B. (eds.), Virus Taxonomy: The Classification and Nomenclature of Viruses. The Seventh Report of the International Committee on Taxonomy of Viruses, Academic Press, SanDiego, 2000, pp. 395-480.
Soo H.M., Handley J.A., Maugeri M.M., Burns P., Smith G.R., Dale J.L., and Harding R.M., J Gen Virol 79, 3155-3361, 1998.
McMahon J.A., Dale J.L., and Harding R.M., Arch Virol 144, 2259-2263, 1999.
Nakashima N., and Noda H., J Gen Virol 75, 2803-2806, 1994.
Isogai M., Uyeda I., Uyeda I., and Lee B.C., J Gen Virol 79, 1487-1494, 1998.
Zhang H.M., Chen J.P., and Adams M.J., Arch Virol 146, 2331-2339, 2001.
Uyeda I., Lee B.C., Ando Y., Suga H., He Y.K., and Isogai M., Methods Mol Biol 81, 65-75, 1998.
Lambden P., Cooke S., Caul E., and Clarke I., J Virol 66, 1817-1822, 1992.
Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403-410, 1990.
Kumar S., Tamura K., Jakobsen I.B., and Nei M. Bioinformatics 17, 1244-1245, 2001.
Anzola J.V., Xu Z.K., Asamizu T., and Nuss D.L., Proc Natl Acad Sci USA 84, 8301-8305, 1987.
Nakashima N., Koizumi M., Watanabe H., and Noda H., J Gen Virol 77, 139-146, 1996.
Landschulz W.H., Johnson P.F., and McKnight S.L., Science 240, 1759-1764, 1988.
Saraste M., Sibbald P.R., and Wittinghofer A., Trends Biochem Sci 15, 430-434, 1990.
Ikeda K., Nagaoka S., Winkler S., Kotani K., Yagi H., Nakanishi K., Miyajima S., Kobayashi J., and Mori H., J Virol 75, 988-995, 2001.
Attoui H., Mohd Jaafar F., Biagini P., Canteloube J., de Micco P., Murphy F., and de Lamballerie X., Arch Virol 147, 533-561, 2002.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
McQualter, R.B., Smith, G.R., Dale, J.L. et al. Molecular Analysis of Fiji Disease Fijivirus Genome Segments 1 and 3. Virus Genes 26, 283–289 (2003). https://doi.org/10.1023/A:1024403431373
Issue Date:
DOI: https://doi.org/10.1023/A:1024403431373