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High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures

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Abstract

In vitro shoot proliferation and bulblet production of garlic (Allium sativum L.) was studied in liquid cultures. Shoots grown in vitro were used as explants and were cultured in MS medium supplemented with 2% (w/v) sucrose and 0.5 mg l−1 2-iP. Three culture methods (semi-solid, liquid-immersion and raft) were compared for shoot proliferation. Explants in liquid (immersion) culture exhibited an increased multiplication rate and fresh weight of shoots after 3 weeks of culture as compared with the other treatments. Bulblet formation and growth were studied in liquid medium with different concentrations of sucrose (2–13%). MS medium containing 11% (w/v) sucrose was optimal for bulblet development and bulblets developed in this medium within 9 weeks in culture. The highest multiplication rate was (135 bulblets/explant) found when explants were cultured in bulbing medium (MS medium containing 0.1 mg l−1 NAA+11% (w/v) sucrose) supplemented with 10 μM JA. Growth retardants CCC, B-9, ABA also promoted induction and growth of bulblets. Darkness promoted the bulblet induction and growth compared to light conditions (16-h photoperiod of 50 μmol m−2 s−1). The dormancy of bulblets was broken by cold treatment at 4 °C for 8 weeks.

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References

  • Abo El-Nil MM(1977) Organogenesis and embryogenesis in callus cultures of garlic (Allium satium L.). Plant Sci. Lett. 9: 259-264

    Google Scholar 

  • Alvard A, Cote F & Teisson C (1993) Comparison of methods of liquid medium culture for banana micropropagation. Effects of temporary immersion of explants. Plant Cell Tiss. Org. Cult. 32: 55-60

    Google Scholar 

  • Ayabe M & Sumi S (1998) Establishment of a novel tissue culture method, stern-disc culture, and its practical application to micro-propagation of garlic (Allium sativum L.). Plant Cell Rep. 17: 773-779

    Google Scholar 

  • Bhojwani SS (1980) In vitro propagation of garlic by shoot proliferation. Sci. Hort. 13: 47-52

    Google Scholar 

  • Dantu PK & Bhojwani SS (1987) In vitro propagation of gladiolus. Gartenbauwissenschaft 52: 90-93

    Google Scholar 

  • Escalona M, Lorenzo JC, Gonzalez B, Daquinta M, Gonzalez JL, Desjardin Y & Borrota CG (1999) Pineapple (Ananas comosus L. Merr.) micropropagation in temporary immersion systems. Plant Cell Rep. 18: 743-748

    Google Scholar 

  • Garner N & Blake J (1989) The induction of potato microtubers in vitro on media free growth regulatory substances. Ann. Bot. 63: 663-674

    Google Scholar 

  • Haque MS, Wada T & Hattori K (1997) High frequency shoot regeneration and plantlets formation from root tip of garlic. Plant Cell Tiss. Org. Cult. 50: 83-89

    Google Scholar 

  • Ilan A, ZIiv M & Halevy AH (1995) Propagation and corm development of Brodiaea in liquid cultures. Sci. Hort. 63: 101-112

    Google Scholar 

  • Koch M, Tanami Z & Salomon R (1995) Improved regeneration of shoots from garlic callus. HortScience 30: 378

    Google Scholar 

  • Myers JM & Simon PW (1998) Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot-tip-derived plantlets. Plant Cell Rep. 17: 726-730

    Google Scholar 

  • Myers JM & Simon PW (1999) Regeneration of garlic callus affected by clonal variation, plant growth regulators and culture conditions over time. Plant Cell Rep. 19: 32-36

    Google Scholar 

  • Mohamed-Yassen Y, Barringer SA & Splittstoesser WE (1995) In vitro bulb production form Alllium spp. In Vitro Cell Dev. Biol. 31: 51-52

    Google Scholar 

  • Moriconi DN, Conci VC & Nome SF (1990) Rapid multiplication of garlic (Allium sativum L.) in vitro. Phyton 51: 145-151

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497

    Google Scholar 

  • Nagakubo T, Nagasawa A & Ohkawa H (1993) Micropropagation of garlic through in vitro bulblet formation. Plant Cell Tiss. Org. Cult. 32: 175-183

    Google Scholar 

  • Nagasawa A & Finer JJ (1988) Induction of morphogenic callus cultures from leaf tissue of garlic. HortScience 23: 1068-1070

    Google Scholar 

  • Nimmi Y & Onozawa T (1979) In vitro bulblet from leaf segments of lilies, especially Lilium rubellum Baker. Sci. Hort. 11: 379-389

    Google Scholar 

  • Nojiri H, Yamane H, Seto H, Yamguch I, Morofushi N, Yoshihara T & Shibaoka H (1992) Qualitative and quantitative analysis of endogenous jasmonic acid in bulbing and non-bulbing onion plants. Plant Cell Physiol. 33: 1225-1231

    Google Scholar 

  • Paek KY & Murthy HN (2002) High frequency of bulblet regenera-tion from bulb scale section of Fritillaria thunbergii. Plant Cell Tiss. Org. Cult. 68: 247-252

    Google Scholar 

  • Ravnikar M, Zel J, Plaper I & Spacapan A (1993) Jasmonic acid stimulates shoot and bulb formation in garlic in vitro. Plant Growth Regul. 12: 73-77

    Google Scholar 

  • Santos I & Salema R (2000) Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul. 30: 133-138

    Google Scholar 

  • Seabrook JEA (1994) In vitro propagation and bulb formation of garlic. Can. J. Plant. Sci. 74: 155-158

    Google Scholar 

  • Skidmore DT, Simon AJ & Bedi S (1988) In vitro culture of shoots of Pinus caribaea on a liquid medium. Plant Cell Tiss. Org. Cult. 14: 129-136

    Google Scholar 

  • Stimart DP & Ascher PD (1978) Tissue culture of bulb scale sections for asexual propagation of Lilium longiflorum Thumb. J. Am. Soc. Hort. Sci. 103: 180-184

    Google Scholar 

  • Stimart DP, Ascher PD & Wilkins HF (1982) Overcoming dor-mancy of Lilium longiflorum bulblets produced by tissue culture. J. Am. Soc. Hort. Sci. 107: 1004-1007

    Google Scholar 

  • Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants. Plant Cell Tiss. Org. Cult. 17: 101-110

    Google Scholar 

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, South Korea

    E.K. Kim, E.J. Hahn, H.N. Murthy & K.Y. Paek

  2. Department of Botany, Karnatak University, Dharwad, 580 003, India

    H.N. Murthy

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  1. E.K. Kim
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  2. E.J. Hahn
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  3. H.N. Murthy
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  4. K.Y. Paek
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Correspondence to K.Y. Paek.

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Kim, E., Hahn, E., Murthy, H. et al. High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures. Plant Cell, Tissue and Organ Culture 73, 231–236 (2003). https://doi.org/10.1023/A:1023029302462

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