Abstract
Random amplified polymorphic DNA (RAPD) markers were used to evaluatethe levels and patterns of genetic diversity in ten Elymusalaskanus populations, which were collected from Canada, USA,Greenland and Russia. Ten arbitrarily chosen decamer primers were used in thisstudy. The results revealed high levels of variation. The mean number of allelesper locus (Ap) was 1.5, ranging from 1.4 to 1.6, the meanpercent of polymorphic loci (Pp) was 49.5%, ranging from35.1% to 64.9%, and the mean gene diversity (Hep) was0.162, varying from 0.142 to 0.262. The total variation wasH T = 0.403. When partitioned(G ST), 60% of the total variation was foundamong the populations. Although the genetic diversity values obtained with RAPDsare much higher than for allozymes, they are similar regarding how the geneticvariation is distributed among populations. In addition, a similar geneticpattern of population differentiation, where populations from Greenland andthe USA (“violaceus” and “latiglumis”)were clearly separated from the others (“hyperarcticus”,“komarovii” and “sajanensis”), wasrevealed by both the cluster and principal coordinates analyses.
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Åbo-elwafa A., Murai K. and Shimada T. 1995. Intra-and Inter-specific variations in Lens revealed by RAPD markers. Theor. Appl. Genet. 90: 335–340.
Ayana A., Bryngelsson T. and Bekele E. 2000. Genetic variation of Ethiopian and Eritrean sorghum (Sorghum biocolor (L.) Moench) germplasm assessed by random amplified polymorphic DNA (RAPD). Genet. Resour. Crop Evol. 47: 471–482.
Barkworth M. 1996. The Elymus trachycaulus complex in North America: More question than answer. In: Wang R.R.-C., Jensen L.B. and Jaussi C. (eds), Proc. Second International Triticeae Symposium. Logan, Utah, USA, pp. 189–206.
Barkworth M. 1997 Elymus alaskanus. Nomenclatural set. Internet address: http: / / herbarium.usu.edu /openherbarium/ elymalnm.html
Bartish I.V., Garkava L.P. and Rumpuunen K. 2000. Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theor. Appl. Genet. 101: 554–563.
Baum B.R., Yen C. and Yang J.L. 1991. Roegneria: its generic limits and justification for its recognition. Can. J. Bot. 69: 282–294.
Cao W., Scoles G., Hucl P. and Chibbar R.N. 1999. The use of RAPD analysis to classify Triticum accessions. Theor. Appl. Genet. 98: 602–607.
Chan K.F. and Sun M. 1997. Genetic diversity and relationship detected by isozyme and RAPD analysis of crop and wild species of Amaranthus. Theor. Appl. Genet. 95: 865–873.
Cody W.J. 1996. Flora of the Yukon Territory. NRC Research Press, Ottawa, Canada.
Dawson I.K., Chalmers K.J., Waugh R. and Powell W. 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers. Mol. Ecol. 2: 151–159.
Devos K.M. and Gale M.D. 1992. The use of random amplified polymorphic DNA markers in wheat. Theor. Appl. Genet. 84: 567–572.
Díaz O., Salomon B. and von Bothmer R. 1999. Genetic variation and differentiation in Nordic populations of Elymus alaskanus (Scrib. Ex Merr.) Löve (Poaceae). Theor. Appl. Genet. 99: 210–217.
Díaz O., Salomon B. and von Bothmer R. 2000. Levels and distribution of allozyme and RAPD variation in populations of Elymus fibrosus (Poaceae). Genet. Resour. Crop Evol. 47: 11–24.
Fahima T., Sun G.L., Beharav A., Krugman T., Beiles A. and Nevo E. 1998. RAPD polymorphism of wild wheat populations, Triticum dicoccoides, in Israel. Theor. Appl. Genet. 98: 434–447.
Ferguson M.E., Newbury H.J., Maxted N., Ford-Lloyd B.V. and Robertson L.D. 1998. Population genetic structure in Lens taxa revealed by isozyme and RAPD analysis. Genet. Resour. Crop Evol. 45: 549–559.
Gehrig H.H., Rosicke H. and Kluge M. 1997. Detection of DNA polymorphisms in the genus Kalanchoe by RAPD — PCR fingerprint and its relationships to infrageneric taxonomic positon and ecophysiological photosynthetic behaviour of the species. Plant Science 125: 41–51.
Gower J.C. 1966. Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 53: 325–338.
Hamrick J.L. and Godt M.J.W. 1989. Allozyme diversity in plant species. In: Brown A.H.D., Clegg M.T., Kahler A.L. and Weir B.S. (eds), Plant population genetics, breeding and genetic resources. Sinauer Associates Inc., Sunderland, Massachusetts, USA, pp. 43–63.
Heun M., Murphy J.P. and Phillips T.D. 1994. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions. Theor. Appl. Genet. 87: 689–696.
Hauser L.A. and Crovello T.J. 1982. Numerical analysis of generic relationships in Thelypoieae (Brassicaceae). Syst. Bot. 7: 249–268.
Jaaska V. 1992. Isoenzyme variation in the grass genus Elymus-(Poaceae). Hereditas 117: 11–22.
Joshi C.P. and Nguyen H.T. 1993. Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetrapoloid wheats. Genome 36: 602–609.
Lanner-Herrera C., Gustafsson M., Falt A.S. and Bryngelsson T. 1996. Diversity in natural populations of wild Brassica oleracea as estimated by isozyme and RAPD analysis. Genet. Resour. Crop Evol. 43: 13–23.
Lesica P. and Allendorf F.W. 1994. When are peripheral populations valuable for conservation? Conserv. Biol. 9: 753–760.
Liu Z. and Furnier G.R. 1993. Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. Theor. Appl. Genet. 87: 97–105.
Loveless M.D. and Hamrick J.L. 1984. Ecological determinants of genetic structure in plant populations. Ann. Rev. Ecol. Syst. 15: 65–96.
Love A. 1984. Conspectus of the Triticeae. Feddes Rep. 95: 425–521.
Lynch M. 1994. Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 91–99.
Melderis A. 1978. Taxonomic notes on the tribe Triticeae (Gramineae), with special reference to the genera Elymus L. sensu lato, and Agropyron Gaertner sensu lato. Bot. J. Linn. Soc. 76: 368–384.
Muralidharan K. and Wakeland E.K. 1993. Concentration of primer and template qualitatively affects products in random amplified polymorphic DNA PCR. Bio. Techniques 14: 362–364.
Nebury H.J. and Ford-Lloyd B.V. 1993. The use of RAPD for assessing variation in plants. Plant Growth Reg. 12: 413–451.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.
Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.
Nevo E. 1998. Genetic diversity in wild cereals: regional and local studies and their bearing on conservation ex situ and in situ. Genet. Resour. Crop Evol. 45: 355–370.
Newbury H.J. and Ford-Lloyd B.V. 1993. The use of RAPD for assessing variation in plants. Plant Growth Reg. 12: 413–451.
Rieseberg L.H. 1996. Homology among RAPD fragments in interspecific comparision. Mol. Ecol. 5: 99–105.
Rohlf F.J. 1993. Numerical taxonomy and multivariate analysis system. Exeter Software, Setauket, New York, Version 1.80.
Russell J.R., Fuller J.D., Macaulay M., Hatz B.G., Jahoor A., Powell W. et al. 1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor. Appl. Genet. 95: 714–732.
Schilighting C.D. 1986. The evolution of phenotypic plasticity in plants. Ann. Rev. Ecol. Syst. 17: 667–693.
Schoen D.J. and Brown H.D. 1991. Intraspecific variation in population gene diversity and effective population size correlates with the mating system in plants. Proc. Natl. Acad. Sci. USA 88: 4494–4497.
Slatkin M. 1987. Gene flow and the geographic structure of natural populations. Science 236: 778–792.
Slatkin M. and Barton N.H. 1989. A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43: 1349–1368.
Sneath P.H. and Sokal R.M. 1973. Numerical Taxonomy. The Principles and Practice of Numerical Classifications. Freeman, San Francisco.
Sun G.L., Salomon B. and von Bothmer R. 1997. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers. Genome 40: 806–814.
Sun G.L., Salomon B. and von Bothmer R. 1998a. Characterization and analysis of microsatellite loci in Elymus caninus (Triticeae:Poaceae). Theor. Appl. Genet. 96: 676–682.
Sun G.L., Salomon B. and von Bothmer R. 1998b. Characterization of microsatellite loci from Elymus alaskanus and length polymorphism in several Elymus species (Triticeae:Poaceae). Genome 41: 445–463.
Sun G.L., Díaz O., Salomon B. and von Bothmer R. 1999a. Microsatellite variation and its comparison with allozyme and RAPD variation in Elymus fibrosus (Schrenk) Tzvel. (Poaceae). Hereditas 129: 275–282.
Sun G.L., Díaz O., Salomon B. and von Bothmer R. 1999b. Genetic diversity in Elymus caninus as revealed by isozyme, RAPD and microsatellite markers. Genome 42: 420–431.
Svitashev S., Salomon B., Bryngelsson T. and von Bothmer R. 1996. A study of 28 Elymus species using repetitive DNA sequences. Genome 39: 1093–1101.
Svitashev S., Bryngelsson T., Li X. and Wang R.R.-C. 1998. Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus. Genome 41: 120–128.
Swofford D.L. and Selander R.B. 1989. BIOSYS-1: A computer program for the analysis of the allelic variation in population genetics and biochemical systematics. DL Swofford, Release 1–7 Illinois Nat. Survey, Champaign, II.
Tao Y., Maners J.M., Ludlow M.M. and Hanzel R.G. 1993. DNA polymorphism in grain sorghum (Sorghum bicolor L. Moench). Theor. Appl. Genet. 86: 679–688.
Tassannkajon A., Pongsomboon S., Rimphanitchayakit V., Jarayabhand P. and Boonsaeng V. 1997. Random amplified polymorphic DNA (RAPD) for the determination of genetic variation in wild populations of the Black tiger prawn (Penaeus Monodon) in Thailand. Molec. Mar. Biol. Biotech. 6: 110–115.
Tzvelev N.N. 1976. Tribe 3. Triticeae Dumort. In: Fedorov A.A. (ed.), Poaceae, URSS Leningrad. Nauka Publishing House, pp. 147–181.
Wang C., Scoles G., Hucl P. and Chibbar R.N. 1999. The use of RAPD analysis to classify Triticum accessions. Theor. Appl. Genet. 98: 602–607.
Wei J.Z. and Wang R.R.-C. 1995. Genome and species-specific markers and genome relationships of diploid perennial species in Triticeae based on RAPD analyses. Genome 38: 1230–1236.
Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A. and Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res. 18: 6531–6535.
Williams J.G.K., Hanafey M.K., Rafalski J.A. and Tingey S.V. 1993. Genetic analysis using RAPD markers. Methods Enzymol. 218: 704–740.
Wolf K. and Morgan-Richards M. 1998. PCR markers distinguish Plantago major subspecies. Theor. Appl. Genet. 96: 282–286.
Zhou Y.H., Zheng Y.L., Yang J.L., Yen C. and Jia J.Z. 1999. Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA. Acta Phytotaxonomica Sinica 37: 425–432.
Zhang X.-Q., Salomon B., von Bothmer R. and Díaz O. 2000. Patterns and levels of genetic differentiation in North American populations of the Alaskan wheatgrass complex. Hereditas 133: 123–132.
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Zhang, XQ., Salomon, B. & von Bothmer, R. Application of random amplified polymorphic DNA markers to evaluate intraspecific genetic variation in the Elymus alaskanus complex (Poaceae). Genetic Resources and Crop Evolution 49, 399–409 (2002). https://doi.org/10.1023/A:1020663416938
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DOI: https://doi.org/10.1023/A:1020663416938