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Cloning of a rat α1,3-fucosyltransferase gene: a member of the fucosyltransferase IV family

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Abstract

We report the cloning of a rat α1,3-fucosyltransferase gene (rFuc-T), isolated from a rat genomic library by a PCR-cross-hybridization based cloning approach using primers derived from the conserved region of human α1,3-Fuc-T sequences. Comparison of the rFuc-T predicted amino acid sequence with those of previously cloned human and murine fucosyltransferases showed highest degree of homology to murine Fuc-TIV (87% identity) and human Fuc-TIV (78% identity), with lower homology (41–49% identity) to Fuc-TIII, V, VI, and VII. COS-1 cells transfected with the rFuc-T gene expressed a fucosyltransferase activity with type 2 (Galβ1→4GlcNAc)-containing oligosaccharides and the glycolipid acceptor neolactotetraosyl-ceramide but only low activity with sialylated substrates; the SSEA-1/Lex antigen was detected in transfected cells by immunocytochemistry. Based on these results, we surmise that rFuc-T is a member of the fucosyltransferase IV family. Northern blot analysis with a rFuc-T specific probe indicated a major transcript of 4.2 kb most abundantly expressed in rat spleen; minor transcripts of different sizes were detected in several tissues, including rat brain. Abbreviations: Fuc-T, fucosyltransferase; SSEA-1, stage specific embryonic antigen or Lewisx (Lex); ELFT, ELAM-1 ligand fucosyltransferase; ELAM, endothelial-leukocyte adhesion molecule; nLc4Cer, neolactotetraosylceramide (Galβ1→4GlcNAcβ1→3Galβ1→4Glcβ1→1Cer); NEM, N-ethylmaleimide

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References

  1. Fukuda M (1992) In Cell Surface Carbohydrates in Hematopoietic Cell Differentiation and Malignancy (Fukuda EM, ed.) pp. 127-59. Boca Raton, FL: CRC Press.

    Google Scholar 

  2. Velupillai P, Harn DA (1994) Pro Nat Acad Sci 91: 18-22.

    Article  CAS  Google Scholar 

  3. Fenderson BA, Eddy EM, Hakomori S (1990) Bioassays 12: 173-9.

    Article  CAS  Google Scholar 

  4. Kim YS, Itzkowitz SH, Yuan M, Chung YS, Satake K, Umeyama K, Hakomori S (1988) Cancer Res 48: 475-82.

    PubMed  CAS  Google Scholar 

  5. Marani E, Mai K (1992) Histochem J 24: 852-68.

    Article  PubMed  CAS  Google Scholar 

  6. Mai J, Marani E, Hakomori S (1992) Special Issue: CD15. Histochem J 24: 852-68.

    Google Scholar 

  7. Chou DKH, Suzuki Y, Jungalwala FB (1996) Glycoconj J 13: 1-11.

    Article  Google Scholar 

  8. Wiederschain G, Koul O, McCluer RH (1995) Glycoconj J 12: S9.

    Google Scholar 

  9. Kukowska-Latallo JF, Larsen RD, Nair RP, Lowe JB (1990) Genes Develop 4: 1288-303.

    PubMed  CAS  Google Scholar 

  10. Lowe JB, Kukowska-Latallo JF, Nair RP, Larsen RD, Marks RM, Macher BA, Kelly RJ, Ernst LK (1991) J Bio Chem 266: 17467-77.

    CAS  Google Scholar 

  11. Kumar R, Potwin B, Muller WA, Stanley P (1991) J Biol Chem 266: 21777-83.

    PubMed  CAS  Google Scholar 

  12. Sasaki K, Kurata K, Funayama K, Nagata M, Watanabe E, Ohta S, Hanai N, Nishi T (1994) J Biol Chem 269: 14730-7.

    PubMed  CAS  Google Scholar 

  13. Weston BW, Nair RP, Larsen RD, Lowe JB (1992a) J Biol Chem 267: 4152-60.

    PubMed  CAS  Google Scholar 

  14. Weston BW, Smith PL, Kelly RJ, Lowe JB (1992b) J Biol Chem 267: 24575-84.

    PubMed  CAS  Google Scholar 

  15. Natsuka S, Gersten KM, Zenita K, Kannagi R, Lowe JB (1994) J Biol Chem 269: 16789-94.

    PubMed  CAS  Google Scholar 

  16. Natsuka S, Lowe JB (1994) Current Opinion in Structural Biology 4: 683-91.

    Article  CAS  Google Scholar 

  17. Gersten KM, Natsuka S, Trinchera M, Petryniak B, Kelly RJ, Hiraiwa N, Jenkins NA, Gilbert DJ, Copeland NG, Lowe JB (1995) J Biol Chem 270: 25047-56.

    Article  PubMed  CAS  Google Scholar 

  18. Ozawa M, Muramatsu T (1996) J Biochem 119: 302-8.

    PubMed  CAS  Google Scholar 

  19. Smith PL, Gersten KM, Petryniak B, Kelly RJ, Rogers C, Natsuka Y, Alford III JA, Scheidegger EP, Natsuka S, Lowe JB (1996) J Biol Chem 271: 8250-9.

    Article  PubMed  CAS  Google Scholar 

  20. Mollicone R, Candelier JJ, Mennesson B, Couillin P, Venot AP, Oriol R (1992) Carbohydr Res 228: 265-76.

    Article  PubMed  CAS  Google Scholar 

  21. Sajdel-Sulkowska EM, Smith FI, McCluer RH (1994) Glycobiology 4: 741.

    Google Scholar 

  22. Sajdel-Sulkowska EM, Smith FI, McCluer RH (1995) Glycoconjugate J. 12: 476-7.

    Google Scholar 

  23. Mollicone R, Candelier JJ, Reguigne I, Couillin P, Fletcher A, Oriol R (1994) Transfus Clin Biol 1: 91-7.

    Article  PubMed  CAS  Google Scholar 

  24. Goelz SE, Hession C, Goff D, Griffiths B, Tizard R, Newman B, Chi-Rosso G, Lobb R (1990) Cell 63: 1349-56.

    Article  PubMed  CAS  Google Scholar 

  25. Dodemont HJ, Soriano P, Quax WJ, Ramaekers F, Lensha JA, Groenen MA, Bernardi G, Blemendal H (1982) EMBO J 1: 167-71.

    PubMed  CAS  Google Scholar 

  26. Ko AI, Drager UC, Harn DA (1990) Proc Nat Acad Sci 87: 4159-63.

    Article  PubMed  CAS  Google Scholar 

  27. Goelz S, Kumar R, Potvin B, Sundaram S, Brickelmaier M, Stanley P (1994) J Biol Chem 269: 1033-40.

    PubMed  CAS  Google Scholar 

  28. Folch J, Lees M, Sloane-Stanley GH (1957) J Biol Chem 226: 497-509.

    PubMed  CAS  Google Scholar 

  29. Williams MA, McCluer RH (1980) J Neurochem 35: 1351-61.

    Article  Google Scholar 

  30. Dynan WS (1986) Trends in Genet 2: 196-7.

    Article  CAS  Google Scholar 

  31. Shur BD (1991) Glycobiology 1: 563-75.

    PubMed  CAS  Google Scholar 

  32. Cardullo RA, Armant DR, Millette CF (1989) Biochem 28: 1611-17.

    Article  CAS  Google Scholar 

  33. Raychoudhury SS, Millette CF (1993) Molecular Reproduction and Development 36: 195-202.

    Article  PubMed  CAS  Google Scholar 

  34. DeVries T, Srnka CA, Palcic MM, Swiedler SJ, Vandeneijnden DH, Macher BA (1995) J Biol Chem 270: 8712-22.

    Article  CAS  Google Scholar 

  35. Xu Z, Vo L, Macher BA (1996) J Biol Chem 271: 8818-21.

    Article  PubMed  CAS  Google Scholar 

  36. Howard DR, Fukuda M, Fukuda MN, Stanley P (1987) J Biol Chem 262: 16830-7.

    PubMed  CAS  Google Scholar 

  37. Cameron HS, Szczepaniak D, Weston BW (1995) J Biol Chem 270: 20112-22.

    Article  PubMed  CAS  Google Scholar 

  38. Holmes EH, Xu Z, Sherwood AL, Macher BA (1995) J Biol Chem 270: 8145-51.

    Article  PubMed  CAS  Google Scholar 

  39. Mollicone R, Gibaud A, Francois A, Ratcli¤e M, Oriol R (1990) Eur J Biochem 191: 169-76.

    Article  PubMed  CAS  Google Scholar 

  40. Reguigne I, James MR, Richard CW 3rd, Mollicone R, Seawright A, Lowe JB, Oriol R, Couillin P (1994) Cytogenet Cell Genet 66: 104-6.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Neurology, Harvard Medical School, Boston, MA, 02115, USA

    Elizabeth M Sajdel-Sulkowska, Frances I Smith, Gherman Wiederschain & Robert H McCluer

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  1. Elizabeth M Sajdel-Sulkowska
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  2. Frances I Smith
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  3. Gherman Wiederschain
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  4. Robert H McCluer
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Sajdel-Sulkowska, E.M., Smith, F.I., Wiederschain, G. et al. Cloning of a rat α1,3-fucosyltransferase gene: a member of the fucosyltransferase IV family. Glycoconj J 14, 249–258 (1997). https://doi.org/10.1023/A:1018550023637

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