Abstract
The published principles of computer analysis of genomes and protein sets in taxonomically distant eukaryotes are expounded. The authors developed a search strategy to identify in genomes of such organisms genes and proteins nonhomologous in primary structure but having similar functions in cells dividing by meiosis. This strategy based on the combined principles of genomics, proteomics, and morphometric analysis of subcellular structures was applied to a computer search for genes encoding the proteins of synaptonemal complexes in genomes of Drosophila melanogaster, the nematode Caenorhabditis elegans, and the plant Arabidopsis thaliana. These proteins proved to be functionally similar to their counterparts in yeast Saccharomyces cerevisiae (protein Zip1p) and mammals (protein SCP1).
Similar content being viewed by others
REFERENCES
Goffeau, A., Barrell, B.G., Bussey, H., et al., Life with 6000 Genes, Science, 1996, vol. 274, no. 5287, pp. 563-567.
The C. elegans Sequencing Consortium, Genome Sequence of the Nematode C. elegans: A Platform for Investigating Biology, Science, 1998, vol. 282, pp. 2012-2020.
Adams, M.D., Celniker, S.E., Holt, R.A., et al., The Genome Sequence of Drosophila melanogaster, Science, 2000, vol. 287, pp. 2185-2195.
Myers, E.W., Sutton, G.G., Delcher, A.T., et al., A Whole-Genome Assembly of Drosophila, Science, 2000, vol. 287, pp. 2196-2204.
The Arabidopsis Genome Initiative, Analysis of the Genome Sequence of the Flowering Plant Arabidopsis thaliana, Nature 2000, vol. 408, pp. 796-815.
Chervitz, S.A., Aravind, L., Sherlock, G., et al., Comparison of the Complete Protein Sets of Worm and Yeast: Orthology and Divergence, Science, 1998, vol. 282, pp. 2022-2028.
Rubin, G.M., Yandell, M.D., Wortman, J.R., et al., Comparative Genomics of the Eukaryotes, Science, 2000, vol. 287, pp. 2204-2215.
Kornberg, T.B. and Krasnov, M.A., The Drosophila Genome Sequence: Implication for Biology and Medicine, Science, 2000, vol. 287, pp. 2218-2220.
Timofeeff-Ressovsky, N.W. and Vogt, O., Ñber idiosomatishe Variationsgruppen und ihre Bedeutung für Dise Klassifikation der Krankheiten, Naturwiss., 1926, vol. 14, nos. 50-51, pp. 1188-1190.
Muller, H.J., Studies in Genetics, Bloomington: Indiana Univ. Press, 1962.
Bogdanov, Yu.F., The Molecular Concept of Meiosis Withstands a Test: Results of the Fourth European Conference on Meiosis, Genetika (Moscow), 2000, vol. 36, no. 4, pp. 585-590.
Grishaeva, T.M. and Bogdanov, Yu.F., The Genetic Control of Meiosis in Drosophila, Genetika (Moscow), 2000, vol. 36, no. 10, pp. 1301-1321.
Zickler, D. and Kleckner, N., Meiotic Chromosomes: Integrating Structure and Function, Annu. Rev. Genet., 1999, vol. 33, pp. 603-754.
Meuwissen, R.L.J., Offenberg, H.H., Dietrich, A.J.J., et al., A Coiled-Coil Related Protein Specific for the Synapsed Regions of Meiotic Prophase Chromosomes, EMBO J., 1992, vol. 11, pp. 5091-5100.
Dobson, M.J., Pearlman, R.E., Karaiskakis, A., et al., Synaptonemal Complex Proteins: Occurrence, Epitope Mapping and Chromosome Disjunction, J. Cell. Sci., 1994, vol. 107, pp. 2749-2760.
Liu, J.G., Yuan, L., Brundell, E., et al., Localization of the N-Terminus of SCP1 to the Central Element of the Synaptonemal Complex and Evidence for Direct Inter-actions between the N-Termini of SCP1 Molecules Organized Head-to-Head, Exp. Cell Res., 1996, vol. 226, pp. 11-19.
Meuwissen, R.L.J., Meerts, I., Hoovers, J.M.N., et al., Human Synaptonemal Complex Protein 1 (SCP1): Isolation and Characterization of the cDNA and Chromosomal Localization of the Gene, Genomics, 1997, vol. 39, pp. 377-384.
Tung, K.S. and Roeder, G.S., Meiotic Chromosome Morphology and Behavior in zip1 Mutants of Saccharomyces cerevisiae, Genetics, 1998, vol. 149, pp. 817-832.
Dong, H. and Roeder, G.S., Organization of the Yeast Zip 1 Protein within the Central Region of the Synap-tonemal Complex, J. Cell Biol., 2000, vol. 148, no. 3, pp. 417-426.
Heyting, C., Synaptonemal Complex: Structure and Function, Curr. Opin. Cell Biol., 1996, vol. 8, pp. 389-396.
Penkina, M.V., Karpova, O.I., and Bogdanov, Yu.F., Synaptonemal Complex Proteins: Specific Proteins of Meiotic Chromosomes, Mol. Biol. (Moscow), 2002, vol. 36, no. 1, pp. 1-11.
Schmekel, K., Meuwissen, R.L., Dietrich, A.J., et al., Organization of SCP1 Protein Molecules within Synaptonemal Complexes of the Rat, Exp. Cell Res. 1996, vol. 226, no. 1, pp. 20-30.
Bogdanov, Yu.F., Grishaeva, T.M., and Dadashev, S.Ya., The Drosophila melanogaster CG17604 Gene May Be a Possible Functional Homolog of the ZIP1 and SCP1 (SYCP1) Genes Coding for Synaptonemal Complex Proteins, Genetika (Moscow), 2002, vol. 38, no. 1, pp. 108-112.
Matsubayashi, H. and Yamamoto, M.-T., Dissection of Chromosome Region 89A of Drosophila melanogaster by Local Transposition of P Elements, Genes Genet. Syst. 1998, vol. 73, pp. 95-103.
Smith, P.A. and King, R.C., Genetic Control of Synaptonemal Complexes in Drosophila melanogaster, Genetics 1968, vol. 60, pp. 335-351.
FlyBase Drosophila Database, http://flybase.bio. indiana.edu.
Carpenter, A.T.C., Electron Microscopy of Meiosis in Drosophila melanogaster Females. I. Structure, Arrangement and Temporal Change of the Synaptonemal Complex in Wild Type, Chromosoma, 1975, vol. 51, pp. 157-182.
Wayson, S.M., Page, S.L., Carey, B.W., et al., The Role of c(3)G in Promoting Meiotic Chromosome Synapsis and Exchange, The 17th European Drosophila Research Conference (1-5 September 2001), Edinburgh, 2001, p. 34.
Page, S.L. and Hawley, R.S., c(3)G Encodes a Drosophila Synaptonemal Complex Protein, Genes Dev., 2001, vol. 15, no. 23, pp. 3130-3143.
MacQueen, A.J., Colaiacovo, M.P., and Villeneuve, A.M., Molecules Underlying Meiotic Nuclear Reorganization and the Homolog Pairing Process, International C. elegans Meeting 2001, p. 291.
Goldstein, P., The Synaptonemal Complex of Caenorhabditis elegans: Pachytene Karyotype Analysis of the dp1 Mutant and Disjunction Regulator Regions, Chromosoma, 1985, vol. 93, pp. 177-182.
Dernburg, A.F., McDonald, K., Moulder, G., et al., Meiotic Recombination in C. elegans Initiates by a Conserved Mechanism and Is Dispensable for Homologous Chromosome Synapsis, Cell (Cambridge, Mass.), 1998, vol. 94, pp. 387-309.
Schmekel, K. and Daneholt, B., The Central Region of the Synaptonemal Complex Revealed in Three Dimensions, Trends Cell Biol. 1995, vol. 5, pp. 239-242.
Bogdanov, Yu.F., Homologous Series of Meiotic Characters: Evolution and Conservation, Evolyutsiya, ekologiya, bioraznoobrazie. Materialy konferentsii pamyati Nikolaya Nikolaevicha Vorontsova, 1934-2000 (Evolution, Ecology, Biodiversity: Proc. Conf. in Memory of Nikolai Nikolaevich Vorontsov, 1934-2000), Krasilov, V.A., Ed., Moscow: Univ. Tsentr Dovuzovskogo Obrazovaniya, 2001, pp. 60-75.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bogdanov, Y.F., Dadashev, S.Y. & Grishaeva, T.M. Comparative Genomics and Proteomics of Drosophila, Brenner's Nematode, and Arabidopsis: Identification of Functionally Similar Genes and Proteins of Meiotic Chromosome Synapsis. Russian Journal of Genetics 38, 908–917 (2002). https://doi.org/10.1023/A:1016883711260
Issue Date:
DOI: https://doi.org/10.1023/A:1016883711260