Abstract
An improved micropropagation protocol has been developed for teak (Tectona grandis). Nodal explants placed on MS medium supplemented with 22.2 μM benzylaminopurine and then serially transferred to fresh medium after 12, 24, 48 and 72 h gave maximum culture establishment (76.8%). Establishment was reduced when explants were retained in the initial culture medium longer than 12 h. Explants collected in May showed maximum (76.8%) response. Placement of the explants on MS medium supplemented with 22.2 μM benzylaminopurine and 0.57 μM indole-3-acetic acid resulted in the maximum average number of shoots. In vitro raised micro shoots were rooted ex vitro by dipping in indole-3-butyric acid (9.8 mM) for 2 min followed by planting in polyethylene pots containing a soil:vermiculite (1:1 v/v) mixture. This treatment resulted in 77.9% survival of the plantlets. They were weaned in a glasshouse and finally moved to an agro-net shade house.
Similar content being viewed by others
References
Anon. (1996) The Wealth of India, Vol. X, Pub. CSIR, New Delhi, India
Chalupa V (1987) European hardwoods. In: JM Bonga & DJ Durzan (eds.) Cell and Tissue Culture in Forestry, Vol. 3 (pp 224–240). Martinus Nijhoff, Dordrecht
Devi YS, Mukherjee BB & Gupta S (1994) Rapid cloning of elite teak (Tectona grandis Linn.) by in vitro multiple shoot production. Indian J. Exp. Bio. 32: 668–671
Gill RIS & Gill SS (1994) In vitro exudation of phenol in Eucalyptus. Indian Forester 120: 504–509
Gupta PK, Nadgir Al, Mascarenhas AF & Jaganathan V (1980) Tissue culture of forest trees - Clonal multiplication of Tectona grandis (teak) by tissue culture. Plant Sci. Lett. 17: 259–268
Mascarenhas AF, Kendurkar SV, Gupta PK, Khusepe SS & Agrawal DC (1987) Teak. In: JM Bonga & DJ Durzan (eds.) Cell and Tissue Culture in Forestry, Vol. 3 (pp 300–315) Martinus Nijhoff, Dordrecht, Netherlands.
Maslekar AR (1981) Forester's Companion. India Forestry Handbook. Jugal Kishore & Co., India
Moncousin CH (1991) Rooting of in vitro cuttings. In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry, Vol. 17. High-Tech and Micropropagation. Springer-Verlag, Berlin, Heidelberg, Germany
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nautiyal S, Singh U & Gurumurthi K (1992) Rooting response of branch cuttings of teak (Tectona grandis) as influenced by season and growth hormones. Indian Forester 118: 112–121
Nemeth G (1986) Induction of rooting. In: YPS Bajaj (ed) Biotechnology in Agriculture and Forestry Vol. 1: Trees I (pp 49–64). Springer-Verlag, Berlin, Germany
Siril EA & Dhar U (1997) Micropropagation of mature Chinese tallow tree (Sapium sebiferum Roxb.). Plant Cell Rep. 16: 637–640
Tiwari KP, Sharma MC & Tiwari SK (1997) Tissue culture protocols for Teak (Tectona grandis, Neem (Azadirachta indica) and Khamer (Gmelina arboreta). SFRI Technical Bulletin No. 29, State Forest Research Institute, Jabalpur, India
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tiwari, S., Tiwari, K. & Siril, E. An improved micropropagation protocol for teak. Plant Cell, Tissue and Organ Culture 71, 1–6 (2002). https://doi.org/10.1023/A:1016570000846
Issue Date:
DOI: https://doi.org/10.1023/A:1016570000846