Abstract
When immobilized in sol-gels, invertase (β;-fructofuranosidase) from Candida utilis and β;-glucosidase from Pyrococcus furiosus had activity recovery values of 30 and 28%, respectively. However, if Blue Dextran (0.04%) was included in the immobilization-reaction mixture, the respective recovery values increased to 63 and 52%. Glucose dehydrogenase from Thermoplasma acidophilum immobilized by the same method lost most of its activity and Blue Dextran had no effect on the recovery of activity during the immobilization procedure. The immobilized enzymes required treatment with glutaraldehyde in order to maintain their activity within the sol-gel matrix during continuous reaction with their respective substrates.
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O'Neill, H., Angley, C.V., Hemery, I. et al. Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: stabilization of invertase and β;-glucosidase by Blue Dextran** . Biotechnology Letters 24, 783–790 (2002). https://doi.org/10.1023/A:1015572020633
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DOI: https://doi.org/10.1023/A:1015572020633