Abstract
An automated reverse flow injection analysis (r-FIA) system using stop-flow technique for quantifying methanol based on the enzymatic reactions of alcohol oxidase and peroxidase was developed. The system permitted methanol analysis in a linear range of 0.006-0.1 g methanol l−1 without external dilution, and with a sampling frequency of 12 analyses per hour, with a relative standard deviation of 1.16%. The analyser was validated analysing samples from a Pichia pastoris fermentation producing a heterologous protein.
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Almuzara, C., Cos, O., Baeza, M. et al. Methanol determination in Pichia pastoris cultures by flow injection analysis . Biotechnology Letters 24, 413–417 (2002). https://doi.org/10.1023/A:1014554324117
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DOI: https://doi.org/10.1023/A:1014554324117