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Characterization of proteolytic activity of proteases

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Abstract

Methods for characterization of protease activity were applied to three Bacillus sp. protease preparations of varying purity. Activities differed by several orders of magnitude between the three proteases. Fractionation by HPLC and assay of peak activity against the two substrates elucidated the presence of 1–4 active fractions in each protease preparation.

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Authors and Affiliations

  1. Strategic Technology Development Section, Land O'Lakes, Inc., P.O. Box 64101, St. Paul, MN, 55164, USA

    Marc A. Bjurlin, Scott Bloomer & Cara J. Nelson

  2. BioLink, College of Biological Sciences, University of Minnesota, 123 Snyder Hall, 1475 Gortner Ave., St. Paul, MN, 55108

    Marc A. Bjurlin

  3. Department of Philosophy, University of Minnesota, 831 Heller Hall, 271 19th Ave. So., Minneapolis, MN, 55455, USA

    Marc A. Bjurlin

Authors
  1. Marc A. Bjurlin
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  2. Scott Bloomer
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  3. Cara J. Nelson
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Bjurlin, M.A., Bloomer, S. & Nelson, C.J. Characterization of proteolytic activity of proteases. Biotechnology Letters 24, 191–195 (2002). https://doi.org/10.1023/A:1014153122933

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  • DOI: https://doi.org/10.1023/A:1014153122933

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