Abstract
To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methyl-1-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic–inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85 °C.
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Furukawa, Sy., Ono, T., Ijima, H. et al. Activation of protease by sol-gel entrapment into organically modified hybrid silicates. Biotechnology Letters 24, 13–16 (2002). https://doi.org/10.1023/A:1013876313806
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DOI: https://doi.org/10.1023/A:1013876313806