Abstract
The aim of the project was to develop micropropagation procedures for theheavily exploited and endangered South African trees black stinkwood (Ocotea bullata) and pepperbark (Warburgia salutaris) to facilitateconservation and reforestation. Both species are difficult to establish andgrow in tissue culture because of their high phenolic content. A protocol forthe establishment of explants in vitro was developed comprisingdecontamination, the application of antioxidants and cold treatment.
References
Cunningham AB and Ihlenfeldt HD (1990) People and medicines: the exploitation and conservation of traditional Zulu medicinal plants. Proceedings Twelfth Plenary Meeting AETFAT, Hamburg, 1988. Mitteilungen aus dem Institut für Allgemeine Botanik, Hamburg 23b: 979–990
David JM, Yoshida M and Gottlieb OR (1994) Phenylpropanoid, catechins from bark of Ocotea porosa. Phytochemistry 35: 545–546
Dry DH (1991) A breakthrough in the vegetative propagation of Ocotea bullata at the Pretoria National Botanical Gardens. S Afr For J 159: 65–69
Farnsworth K and Gaum WG (1995) Root initiation in Ocotea bullata (Burch.) Baill. cuttings. S Afr For J 173: 31–33
Garcez WS, Yoshida M and Gottlieb OR (1995) Benzylisoquinoline alkaloids and flavonols from Ocotea vellosiana. Phytochemistry 39: 815–816
George EF (1993) Plant Propagation by Tissue Culture, Part I: The Technology. Edington, UK Exegetics Ltd
Himejima M and Kubo I (1992) Antimicrobial agents from Licaria puchuri-major and their synergistic effect with polygodial. J Nat Prod 55: 620–625
Hollmann J and Van der Schijff M (1996) Portrait of a medicinal tree. Veld & Flora 82: 115–116
Hutchings A, Scott AH, Lewis G and Cunningham AB (1996) Zulu Medicinal Plants - An Inventory. University of Natal Press 204–205
Johnson D and Scott-Shaw R (1995) The pepperbark tree of Zululand. Veld & Flora 81: 16
Kowalski B and Van Staden J (1998) Cold treatment, as part of the process, improves explant decontamination. Plant Growth Regul 26: 203–205
Lubbe WA (1990) Management of the coppice regeneration of Ocotea bullat (Lauraceae). S Afr For J 154: 1–6
Lloyd GB and McCown BH (1981) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb Proc Int Plant Prop Soc 30: 421–427
Maura-Costa PH, Viana AM and Mantell SH (1993). In vitro plantlet regeneration of Ocotea catharinensis, an endangered Brazilian hardwood forest tree. Plant Cell Tissue Org Cult 35: 279–286
Murashige T and Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497
Silva ML da, Maia JGS, Rezende CMA da and Gottlieb OR (1973) Arylpropanoids from Licaria puchuri-major. Phytochemistry 12: 471–472
Taniguchi M and Kubo I (1993) Ethnobotanical drug discovery based on medicine men's trials in the African savanna: screening of East African plants for antimicrobial activity II.J Nat Prod 56: 1539–1546
Vilegas JHY, Gottlieb OR, Kaplan MAC and Gottlieb HE (1989) Aprophine alkaloids from Ocotea caesia [stemwood]. Phytochemistry 28: 3577–3578
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kowalski, B., Staden, J.v. In vitro culture of two threatened South African medicinal trees – Ocotea bullata and Warburgia salutaris. Plant Growth Regulation 34, 223–228 (2001). https://doi.org/10.1023/A:1013362615531
Issue Date:
DOI: https://doi.org/10.1023/A:1013362615531