Skip to main content
SpringerLink
Log in

The effects of exogenous proline and proline analogues on in vitro shoot organogenesis in Arabidopsis

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

In vitro shoot organogenesis from Arabidopsis hypocotyl explants was stimulated by 1 mMproline, and to a lesser extent by 5 mM proline, butinhibited by inclusion of 10 mM proline in thehormonally-supplemented regeneration medium. Theability of low concentrations of the prolineanalogues, azetidine-2-carboxylate and thioproline toovercome the stimulatory effect of 1 mM proline, anda slight increase in the stimulative effects of 1 mMproline by D-proline, are consistent with an importantrole for the interconversions of proline and itsprecursors in regulating cell division anddifferentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Akama K, Shiraishi H, Ohta S, Nakamura K, Okada K and Shimura Y (1992) Efficient transformation of Arabidopsis thaliana: Comparison of the efficiencies with various organs, plant ecotype and Agrobacterium strains. Plant Cell Rep 12: 7–11

    Google Scholar 

  2. Chen SL and Kao CH (1995) Cd induced changes in proline level and peroxidase activity in roots of rice seedlings. Plant Growth Regul 17: 67–71

    Google Scholar 

  3. Downes BP and Crowell DN (1998) Cytokinin regulates the expression of a soybean b-expansin gene by a posttranscriptional mechanism. Plant Mol Biol 37: 437–444

    Google Scholar 

  4. Elthon TE and Stewart CR (1984) Effects of the proline analogue L-thiazoline-4-carboxylic acid on proline metabolism. Plant Physiol 74: 213–218

    Google Scholar 

  5. Fleming AJ, McQueen-Mason S, Mandel T and Kuhlemeier C (1997) Induction of leaf primordia by the cell wall protein expansin. Science 276: 1415–1418

    Google Scholar 

  6. Gahan PB, McGarry A, Wang L, Doré T and Carmignac DF (1997) Glucose-6-phosphate and UDP-D-glucose dehydrogenases: Possible markers of vascular differentiation. Phytochem Analysis 8: 110–114

    Google Scholar 

  7. Garcia-Rios M, Fujita T, LaRosa PC, Locy RD, Clithero JM, Bressan RA and Csonka LN (1997) Cloning of a polycistronic cDNA from tomato encoding γ-glutamyl kinase and γ-glutamyl phosphate reductase. Proc Natl Acad Sci USA 94: 8249–8254

    Google Scholar 

  8. Hare PD (1998) A Regulatory Role for Proline Metabolism in Arabidopsis thaliana (L.) Heynh. PhD Thesis, University of Natal, Pietermaritzburg, South Africa

    Google Scholar 

  9. Hare PD and Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21: 79–102

    Google Scholar 

  10. Hare PD, Cress WA and van Staden J (1998) Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ 21: 535–553

    Google Scholar 

  11. Hare PD, Cress WA and van Staden J (1999) Proline synthesis and degradation: A model system for elucidating stress-related signal transduction. J Exp Bot 50: 413–434

    Google Scholar 

  12. Kay LE and Basile DV (1987) Specific peroxidase isoenzymes are correlated with organogenesis. Plant Physiol 84: 99–105

    Google Scholar 

  13. Kiyosue T, Yoshiba Y, Yamaguchi-Shinozaki K and Shinozaki K (1996) A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis. Plant Cell 8: 1323–1335

    Google Scholar 

  14. Lozovaya V, Gorshkova T, Yablokova E, Zabotina O, Ageeva M, Rumyantseva N, Kolesnichenko E, Waranyuwat A and Widholm J (1996) Callus cell wall phenolics and plant regeneration ability. J Plant Physiol148: 711–717

    Google Scholar 

  15. Milazzo MC, Kellett G, Haynesworth K and Shetty K (1998) Regulation of benzyladenine-induced in vitro shoot organogenesis and endogenous proline in melon (Cucumis melo L.) by exogenous proline, ornithine, and proline analogues.J Agric Food Chem 46: 2402–2406

    Google Scholar 

  16. Murthy BNS, Victor J, Singh RP, Fletcher RA and Saxena PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regul 19: 233–240

    Google Scholar 

  17. Nanjo T, Kobayashi M, Yoshiba Y, Sanada Y, Wada K, Tsukaya H, Kakubari Y, Yamaguchi-Shinozaki K and Shinozaki K (1999) Biological functions of proline inmorphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana. Plant J 18: 185–193

    Google Scholar 

  18. Ozawa S, Yasutani I, Fukuda H, Komamine A and Sugiyama M (1998) Organogenic responses in tissue culture of srd mutants of Arabidopsis thaliana. Development 125: 135–142

    Google Scholar 

  19. Trigiano RN and Conger B (1987) Regulation of growth and somatic embryogenesis by proline and serine in suspension cultures of Dactylis glomerata. J Plant Physiol130: 49–55

    Google Scholar 

  20. Verbruggen N, Borstlap AC, Jacobs M, van Montagu M and Messens E (1996) The raz1 mutant of Arabidopsis thaliana lacks the activity of a high-affinity amino acid transporter. Planta200: 247–253

    Google Scholar 

  21. Yang R and Shetty K (1998) Stimulation of rosmarinic acid in shoot cultures of oregano (Oreganum vulgare) clonal line in response to proline, proline analogues, and proline precursors. J Agric Food Biochem 46: 2888–2893

    Google Scholar 

  22. Zhang C-s, Lu Q and Verma DPS (1995) Removal of feedback inhibition of 1-pyrroline-5-carboxylate synthetase, a bifunctional enzyme catalysing the first two steps of proline biosynthesis in plants. J Biol Chem 270: 20491–20496

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    P.D. Hare & W.A. Cress

  2. Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    J. van Staden

Authors
  1. P.D. Hare
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W.A. Cress
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. van Staden
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. van Staden.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hare, P., Cress, W. & van Staden, J. The effects of exogenous proline and proline analogues on in vitro shoot organogenesis in Arabidopsis. Plant Growth Regulation 34, 203–207 (2001). https://doi.org/10.1023/A:1013326526875

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1013326526875

Search

Navigation