Abstract
Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Charati SG,Stern SA (1998) Diffusion of gases in silicone polymers: molecular dynamics simulations. Macromolecules 31: 5529-5535.
Cho MH,Wang SS (1990) Practical method for estimating oxygen kinetic and metabolic parameters. Biotechnol. Prog. 6: 164-167.
Finlayson BA (1972) The Method of Weighted Residuals and Variational Principles. New York: Academic Press.
Foust A,Wenzel L,Clump C,Maus L,Anderson L (1980) Principles of Unit Operations, 2nd edn. New York: John Wiley & Sons.
Kessell RHJ,Carr AH (1972) The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue. J. Exp. Bot. 23: 996-1007.
Payne GF,Shuler ML,Brodelius P (1987) Large-scale plant cell culture. In: Lydersen BK, ed. Large Scale Cell Culture Technology. Münich: Hanser Publishers, pp. 195-229.
Pepin MF,Archambault J,Chavarie C,Cormier F (1995) Growth kinetics of Vitis vinifera cell suspension cultures: 1. Shake flask cultures. Biotechnol. Bioeng. 47: 131-138.
Rosenberg MZ (1989) The Hydrodynamic Shear Sensitivity of Suspension Cultured Plant Cells. Ph.D. Thesis, Washington University.
Stroud AH,Secrest D (1966) Gaussian Quadrature Formulas. New Jersey: Prentice-Hall Inc.
Sun X,Linden JC (1999) Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor. J. Ind. Microbiol. Biotechnol. 22: 44-47.
Tate JL,Payne GF (1991) Plant cell growth under different levels of oxygen and carbon dioxide. Plant Cell Rep. 10: 22-25.
Wong V,Williams D,Colby C,Saint DA (2000) Correlation of O2 uptake rate and mitochondrial activity in carrot cells cultures exposed to laminar fluid shear. Biotechnol. Lett. 22: 1919-1924.
Wudtke M,Schugerl K (1987) Investigations of the influence of physical environment on the cultivation of animal cells. In: European Society of Animal Cell Technology, the 8th Meeting Tiberias, Israel. Butterworth & Co, pp. 297-315.
Zhong JJ,Fujiyama K,Seki T,Yoshida T (1994) A quantitative analysis of shear effects on cells suspension and cell culture of Perilla frutescens in bioreactors. Biotechnol. Bioeng. 44: 649-654.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wong, V., Williams, D. & Colby, C.B. Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects. Biotechnology Letters 23, 1841–1847 (2001). https://doi.org/10.1023/A:1012766116568
Issue Date:
DOI: https://doi.org/10.1023/A:1012766116568