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Characterization of Somatotropin- and Prolactin-Binding Sites on Bovine Granulosa Cells Using Homologous Hormones

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Abstract

Specific binding of bovine somatotropin (BST) and bovine prolactin (BPRL) to cow granulosa cells from antrumcontaining follicles of different diameter was studied. Scatchard analysis of the data revealed a single type of low affinity BST-binding sites on the granulosa cells with dissociation constants similar to those for the BPRL-binding sites. The number of BST-binding sites on the cells decreased with increasing follicle diameter from 3-5 to 6-10 mm. However, the binding capacity to BPRL decreased only in the case of cells from follicles 11-20 mm in diameter. The findings are discussed in relation to the “homologous binding” phenomenon.

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Authors and Affiliations

  1. All-Union Research Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Sciences, Moskovskoe Shosse 55a, Pushkin, St. Petersburg, 189620, Russia

    I. Yu. Lebedeva

  2. All-Union Research Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Sciences, Moskovskoe Shosse 55a, Pushkin, St. Petersburg, 189620, Russia

    V. A. Lebedev & T. I. Kuzmina

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  1. I. Yu. Lebedeva
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  2. V. A. Lebedev
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  3. T. I. Kuzmina
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Lebedeva, I.Y., Lebedev, V.A. & Kuzmina, T.I. Characterization of Somatotropin- and Prolactin-Binding Sites on Bovine Granulosa Cells Using Homologous Hormones. Biochemistry (Moscow) 66, 967–972 (2001). https://doi.org/10.1023/A:1012309307153

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