Abstract
We amplified the capsid protein gene fragments of 30 Japanese isolates of feline calicivirus (FCV), including the C, D, and E regions, by reverse transcription-polymerase chain reaction (RT-PCR), followed by direct sequencing. Alignment of the predicted amino acid sequences, together with other published sequences from the isolates obtained in other countries, demonstrated a marked heterogeneity among the isolates, confirming the current definition of hypervariable regions within the capsid protein; these regions give rise to the antigenic variations seen in FCV isolates. Phylogenetic analysis of the nucleotide sequences could not identify significant geographically or temporally separated clusters of FCV isolates, supporting the theory of a single genotype.
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Gillespie J.H. and Scott F.W., Adv Vet Sci Comp Med 17, 163-200, 1973.
Kalunda M., Lee K.M., Holmes D.F., and Gillespie J.H., Am J Vet Res 36, 353-356, 1975.
Povey R.C., Infect Immum 10, 1307-1314, 1974.
Dawson S., McArdle F., Bennett M., Carter M., Milton I.P., Turner P., Meanger J., and Gaskell R.M., Vet Rec 133, 13-17, 1993.
Seal B.S., Ridpath J.F., and Mengeling W.L., J Gen Virol 74, 2519-2527, 1993.
Geissler K., Schuneider K., Platzer G., Truyen B., Oskar-Rüger K. and Truyen U., Virus Res 48, 193-206, 1997.
Seal B.S., Virus Res 33, 39-53, 1994.
Baulch-Brown C., Love D., and Meanger J., Vet Microbiol 68, 107-117, 1999.
Tohya Y., Yokoyama N., Maeda K., Kawaguchi Y., and Mikami T., J Gen Virol 78, 303-305, 1997.
Radford A.D., Willoughby K., Dawson S., McCracken C., and Gaskell R.M., J Virol 73, 8496-8502, 1999.
Baulch-Brown C., Love D., and Meanger J., Aust Vet J 75, 209-213, 1997.
Tajima T., Nakata E., Kawamura H., Mochizuki M., and Uchino T., J Anim Clin Res Found 1, 19-23, 1993 (in Japanese).
Tajima T., Yoshizaki S., Nakata E., Tohya Y., Ishiguro S., Fujikawa Y., and Sugii S., J Vet Med Sci 60, 155-160, 1998.
Takahashi E., Konishi S., and Ogata M., Jpn J Vet Sci 30, 197-206, 1971.
Tohya Y., Taniguchi Y., Takahashi E., Utagawa E., Takeda N., Miyamura K., Yamazaki S., and Mikami T., Virology 183, 801-814, 1991.
Guiver M., Littler E., Caul E.O., and Fox A.J., J Gen Virol 73, 2429-2433, 1992.
Milton I.D., Turner J., Teelan A., Gaskell R., Turner P.C., and Carter M.J., J Gen Virol 73, 2435-2439, 1992.
Tajima T., Nakata E., Tohya Y., Yuri K., Katase H., and Mikami T., J Vet Epidemiol 2, 57-62, 1997.
Glenn M., Radford A.D., Turner P.C., Carter M., Lowery D., DeSilver D.A., Meanger J., Baulch-Brown C., Bennett M., and Gaskell R.M., Vet Microbiol 67, 175-193, 1999.
Radford A.D., Bennett M., McArdle F., Dawson S., Turner P.C., Glenn M.A., and Gaskell R.M., Vaccine 15, 1451-1458, 1997.
Kreutz L.C., Johnson R.P., and Seal B.S., Vet Microbiol 59, 229-236, 1998.
Radford A.D., Turner P.C., Bennett M., McArdle F., Dawson S., Glenn M.A., Williams R.A., and Gaskell R.M., J Gen Virol 79, 1-10, 1998.
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Horimoto, T., Takeda, Y., Iwatsuki-Horimoto, K. et al. Capsid Protein Gene Variation among Feline Calicivirus Isolates*. Virus Genes 23, 171–174 (2001). https://doi.org/10.1023/A:1011892120875
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DOI: https://doi.org/10.1023/A:1011892120875