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Production of Drosophila melanogaster acetylcholinesterase in Saccharomyces cerevisiae

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Abstract

Biologically active acetylcholinesterase from Drosophila melanogaster was constitutively expressed from a high copy number plasmid in Saccharomyces cerevisiae. Cellular metabolism and plasmid copy number were unaffected by expression. Based on comparative PMSF inhibition, the K cat value (6430 mol s−1) was commensurate with human and Torpedo acetylcholinesterases. A 1.7 knt truncated mRNA transcript was observed on Northern blot analysis.

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Authors and Affiliations

  1. School of Biological Sciences, Queen Mary and Westfield College, University of London, Mile End Road, London, E1 4NS, UK

    K.C.A. Stopps, B.P.G. Curran, S.A. Khalawan & C.A. Malcolm

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  1. K.C.A. Stopps
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  2. B.P.G. Curran
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  3. S.A. Khalawan
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  4. C.A. Malcolm
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Correspondence to C.A. Malcolm.

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Stopps, K., Curran, B., Khalawan, S. et al. Production of Drosophila melanogaster acetylcholinesterase in Saccharomyces cerevisiae. Biotechnology Letters 23, 417–422 (2001). https://doi.org/10.1023/A:1010330015598

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  • DOI: https://doi.org/10.1023/A:1010330015598

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