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Streptococcal integration vectors for gene inactivation and cloning

  • Published:
Methods in Cell Science

Abstract

Five streptococcal integration vectors containing different antibiotic resistance-encoding genes capable of expressing in both Streptococcus sp. and Escherichia coli are introduced. These plasmids can replicate in E. coli, but not in streptococci, because the plasmids lack a streptococcal origin of replication. If these plasmids carry a fragment of streptococcal DNA they can specifically integrate into the streptococcal chromosome via Campbell-like, single crossover homologous recombination. Methods are described to use these vectors for gene inactivation and cloning in streptococci.

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Author notes

  1. Lin Tao

    Present address: Department of Oral Biology, College of Dentistry, Chicago, Illinois, USA)

Authors and Affiliations

  1. Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City, Kansas City, Missouri, USA

    Lin Tao

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  1. Lin Tao
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Tao, L. Streptococcal integration vectors for gene inactivation and cloning. Methods Cell Sci 20, 59–64 (1998). https://doi.org/10.1023/A:1009756819641

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  • DOI: https://doi.org/10.1023/A:1009756819641

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