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Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans

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Abstract

Six F1 populations produced from crosses between two Solanum microdontum genotypes and three S. tuberosum genotypes have been assessed for resistance to Phytophthora infestans (late blight; LB) in the field and proved to segregate for P. infestans resistance. The six populations were subjected to genetic mapping using AFLP markers in combination with a core map consisting of RFLPs and a few PCR-based markers. The two S. microdontum parents MCD167 and MCD178 harbour different alleles contributing to resistance. In the MCD167 set of populations a major QTL contributing to resistance was located on chromosome 4. This locus was not detected in the MCD178 set of populations. In the latter set of populations, a major QTL for resistance was detected on chromosome 10. This locus for resistance was not detected in the MCD167 set of populations. A third QTL contributing strongly to resistance was detected on chromosome 5. However, this locus was not consistently detected in all populations. For the loci on chromosome 5 and 10, segregation for resistance alleles from the susceptible parents was also observed. The results provide a starting point for future combinations of genes, to predict the value of each combination and finally to obtain potato germplasm carrying high levels of durable resistance to LB.

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References

  1. Bendahmane A, Kanyuka K, Baulcombe DC: High resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato. Theor Appl Genet 95: 153-162 (1997).

    Google Scholar 

  2. Colon LT, Budding DJ: Resistance to late blight (Phytophthora infestans in ten wild Solanum species. Euphytica volume (suppl): 77-86 (1988).

    Google Scholar 

  3. Colon LT, Jansen RC, Budding DJ: Partial resistance to late blight (Phytophthora infestans) in hybrid progenies of four South American Solanum species crossed with diploid S. tuberosum. Theor Appl Genet 90: 691-698 (1995).

    Google Scholar 

  4. Drenth A, Turkensteen LJ, Govers F: The occurrence of the A2 mating type of Phytophthora infestans in the Netherlands: significance and consequences. Neth J Plant Path 99(suppl. 3): 57-67 (1993).

    Google Scholar 

  5. Drouin G, Dover GA: Independent gene evolution in the potato actin gene family demonstrated by phylogenetic procedures for resolving gene conversions and the phylogeny of angiosperm actin genes. J Mol Evol 31: 132-150 (1990).

    Google Scholar 

  6. Fry WE, Goodwin SB: Recent migrations of Phytophthora infestans. In: Dowley LJ, Bannon E, Cooke LR; Keane O'Sullivan E (Eds), Phytophthora infestans. Publisher Dublin, Boole Press, pp. 89-95 (1993).

  7. Fry WE, Goodwin SB, Dyer AT, Matuszak JM, Drenth A, Tooley PW, Sujkowski LS, Koh YJ, Cohen BA, Spielman LJ, Deahl KL, Inglis DA, Sandlan KP: Historical and recent migrations of Phytophthora infestans: chronology and implications. Plant Dis 77: 653-661 (1993).

    Google Scholar 

  8. Ganal MW, Simon R, Brommonschenkel S, Arndt M, Phillips MS, Tanksley SD, Kumar A: Genetic mapping of a wide spectrum nematode resistance gene (Hero) against Globodera rostochiensis in tomato. Mol Plant-Microbe Interact 8: 886-891 (1995).

    Google Scholar 

  9. Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F: RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78: 65-75 (1989).

    Google Scholar 

  10. Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F: RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83: 49-57 (1991).

    Google Scholar 

  11. Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E: Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85: 541-544 (1993).

    Google Scholar 

  12. Hammond Kosack KE, Jones JDG: Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol Biol 48: 575-607 (1997).

    Google Scholar 

  13. Jacobs JME, van Eck HJ, Horsman K, Arens PFP, Verkerk-Bakker B, Jacbosen E, Pereira A, Stiekema WJ: Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei. Mol Breed 2: 51-60 (1996).

    Google Scholar 

  14. Koh YJ, Goodwin S, Dyer AT, Cohen B, Ogoshi A, Sato N, Fry WE: Migrations and displacements of Phytophthora populations in east Asian countries. Phytopathology 84: 922-927 (1994).

    Google Scholar 

  15. Kreike CM, Stiekema WJ: Reduced recombination and distorted segregation in a Solanum tuberosum (2×) × S. spegazinii (2×) hybrid. Genome 40: 80-87 (1997).

    Google Scholar 

  16. Lander ES, Botstein D: Mapping Mendelian factors underlying quantitative traits using RFLP maps. Genetics 121: 185-199 (1989).

    Google Scholar 

  17. Leister D, Ballvora A, Salamini F, Gebhardt C: A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nature Genet 14: 421-429 (1996).

    Google Scholar 

  18. Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C: Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137: 67-77 (1994).

    Google Scholar 

  19. Li X, van Eck HJ, Rouppe van der Voort J, Huigen DJ, Stam P, Jacobsen E: Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96: 1121-1128 (1998).

    Google Scholar 

  20. Oberhagemann P, Chatot-Balandras C, Schäfer-Pregl R, Wegener D, Palomino C, Salamini F, Bonnel E, Gebhardt C: A genetic analysis of quantitative resistance to late blight in potato towards marker-assisted selection. Mol Breed 5: 399-415 (1999).

    Google Scholar 

  21. Pineda O, Bonierbale MW, Plaisted RL, Brodie BB, Tanksley SD: Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Genome 36: 152-156 (1993).

    Google Scholar 

  22. Provan J, Powell W, Waugh R: Microsatellite analysis of relationships within cultivated potato (Solanum tuberosum). Theor Appl Genet 92: 1078-1084 (1996).

    Google Scholar 

  23. Robertson NF: The challenge of Phytophthora infestans. In: Ingram DS, Williams PH (Eds.), Advances in Plant Pathology, vol. 7, Phytophthora infestans. The cause of Late Blight of potato, pp. 1-30, London Academic Press (1991).

    Google Scholar 

  24. Sandbrink JM, van Ooijen JW, Purimahua CC, Vrielink M, Verkerk R, Zabel P, Lindhout P: Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs. Theor Appl Genet 90: 444-450 (1995).

    Google Scholar 

  25. Shaner G, Finney RE: The effect of nitrogen fertilization on the expression of slow mildewing resistance on Knox wheat. Phytopathology 67: 1051-1056 (1977).

    Google Scholar 

  26. Smulders MJM, Bredemeijer G, Rus-Kortekass W, Arens P, Vosman B: Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species. Theor Appl Genet 97: 264-272 (1997).

    Google Scholar 

  27. Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND: High density molecular linkage map of tomato and potato genomes. Genetics 132: 1141-1160 (1992).

    Google Scholar 

  28. van der Beek JG, Verker R, Zabel P, Lindhout P: Mapping strategy for resistance genes in tomato based on RFLPs between cultivars: Cf9 (resistance to Cladosporium fulvum on chromosome I. Theor Appl Genet 84: 106-112 (1992).

    Google Scholar 

  29. van Eck HJ, Jacobs JME, van Dijk J, Stiekema WJ, Jacobsen E: Identification and mapping of three flower colour loci of potato (S. tuberosum L.) by RFLP analysis. Theor Appl Genet 86: 295-300 (1993).

    Google Scholar 

  30. van Eck HJ, van der Voort JR, Draaistra J, van Zandvoort P, van Enckevort E, Segers B, Peleman J, Jacobsen E, Helder J, Bakker J: The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring. Mol Breed 1: 397-410 (1995).

    Google Scholar 

  31. van Ooijen JW, Maliepaard C: MapQTL (tm) version 3.0: Software for the calculation of QTL positions on genetic maps: CPRO-DLO, Wageningen (1996).

    Google Scholar 

  32. Vos P, Hogers R, Bleeker M, Reijans M, van der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414 (1995).

    Google Scholar 

  33. Wastie RL: Breeding for resistance. In: Ingram DS, Williams PH (Eds.), Advances in Plant Pathology, vol. 7, Phytophthora infestans: The cause of Late Blight of Potato, pp. 193-224, London Academic Press (1991).

    Google Scholar 

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Authors and Affiliations

  1. Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, Netherlands

    Johannes M. Sandbrink, Leontine T. Colon, Petra J.C.C. Wolters & Willem J. Stiekema

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  1. Johannes M. Sandbrink
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  2. Leontine T. Colon
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  3. Petra J.C.C. Wolters
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  4. Willem J. Stiekema
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Sandbrink, J.M., Colon, L.T., Wolters, P.J. et al. Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans. Molecular Breeding 6, 215–225 (2000). https://doi.org/10.1023/A:1009697318518

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