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Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.)

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Abstract

Fusarium wilt caused by Fusarium oxysporum f.sp. melonis is one of the most devastating diseases in melon production worldwide. The most effective control measure available is the use of resistant varieties. Identifying molecular markers linked to resistance genes can serve as a valuable tool for the selection of resistant genotypes. Bulked segregant analysis was used to identify markers linked to the Fom-2 genes, which confers resistance to races 0 and 1 of the fungal pathogen. Pooled DNA from homozygous resistant or homozygous susceptible progeny of F2 cross between MR-1 and AY was screened using 240 PstI/MseI and 200 EcoRI/MseI primer combinations to identify AFLP markers linked to Fom-2. Fifteen markers potentially linked to Fom-2 were identified, all with EcoRI/MseI primer pairs. These were mapped relative to Fom-2 in a backcross (BC) population of 60 progeny derived from MR-1 × AY with AY as recurrent parent. Two AFLP markers (ACT/CAT1 and AAC/CAT1) flanked the gene at 1.7 and 3.3 cM, respectively. Moreover, AFLP marker AGG/CCC and the previously identified RAPD marker 596-1 cosegregated with Fom-2. These two dominant markers were converted to co-dominant markers by designing specific PCR primers that produced product length polymorphisms between the parents. A survey of 45 melon genotypes from diverse geographic origins with the co-dominant markers demonstrated a high correlation between fragment size and the resistance phenotype. These markers may therefore be useful in marker-assisted breeding programs.

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Author notes

  1. Yi-Hong Wang

    Present address: US Plant, Soil and Nutrition Lab, Cornell University, Tower Road, Ithaca, NY , 14853, USA

Authors and Affiliations

  1. Department of Plant Pathology & Physiology and Genomics Institute (CUGI), Clemson University, Clemson, SC , 29634, USA

    Yi-Hong Wang & Ralph A. Dean (Director Fungal Genomics)

  2. USDA, ARS, US Vegetable Laboratory, 2975 Savannah Hwy, Charleston, SC , 29414, USA

    Claude E. Thomas

  3. North Carolina State University, Campus Box 7251, Raleigh, NC , 27695-7251, USA

    Ralph A. Dean (Director Fungal Genomics)

Authors
  1. Yi-Hong Wang
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  2. Claude E. Thomas
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  3. Ralph A. Dean
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Wang, YH., Thomas, C.E. & Dean, R.A. Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.). Molecular Breeding 6, 379–389 (2000). https://doi.org/10.1023/A:1009671925793

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