Abstract
Purpose: Our purpose was to examine the timing ofimplantation and early embryo development following uterinetransfer of oocytes/embryos previously subjected to zonapellucida micromanipulation.
Methods: A total of 68 singleton pregnancies resulting fromIVF and embryo transfer with/without micromanipulation.Patients were divided into four groups according to the typeof micromanipulation technique: assisted hatching, embryobiopsy, intracytoplasmic sperm injection, and nomicromanipulation (control group). Serial serum β-hCG levels weremeasured between 10 and 25 days after fertilization andlog-transformed. Linear regression analyses were performedand extrapolated to hCG = 10 mIU/ml (hCG10) to estimatedetectable implantation. The slopes of the regression lineswere used to estimate the rising speed of hCG, an indirectsign of embryo development.
Results: There were no significant differences among groupswith respect to hCG10, the slopes or intercepts of theregression lines.
Conclusions: Various oocyte/embryo microsurgicalprocedures used in ART involving zona pellucida manipulationdo not appear to affect the timing of implantation or earlyembryo development.
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Hsu, MI., Barroso, G., Mayer, J. et al. Is the Timing of Implantation Affected by Zona Pellucida Micromanipulation?. J Assist Reprod Genet 17, 34–38 (2000). https://doi.org/10.1023/A:1009497929994
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DOI: https://doi.org/10.1023/A:1009497929994