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Molecular Variability of the Capsid Protein of the Prune Dwarf Virus

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Abstract

Sequences of the capsid protein gene and the preceding intergenic region of eleven isolates of prune dwarf virus from central Europe were determined. The isolates were obtained from plum, cherry and peach trees. Comparison of all sequenced isolates (including two sequences published previously) revealed high (88%) conservation of the capsid protein gene. The highest degree of identity was observed in the C-terminal half, where only 13 amino acid substitutions could be observed in contrast to the N-terminal half with 22 substitutions. No reasonable correlation between amino acid substitutions and host species and/or geographic origin of the isolates was observed. Alignment with capsid protein genes of other ilarviruses revealed apple mosaic virus, elm mottle virus, lilac ring mottle virus and prunus necrotic ringspot virus as the most related to prune dwarf virus. Unlike the isolates of related prunus necrotic ringspot virus all the isolates of prune dwarf virus shared extensive conservation of the intergenic region. Portions of RNA3 were selected for design of universal primers for PCR detection.

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Authors and Affiliations

  1. Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Dana Vašková

  2. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ – 37005, České Budějovice, Czech Republic

    Dana Vašková & Josef Špak

  3. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ – 37005, České Budějovice, Czech Republic;

    Karel Petrzik

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Vašková, D., Petrzik, K. & Špak, J. Molecular Variability of the Capsid Protein of the Prune Dwarf Virus. European Journal of Plant Pathology 106, 573–580 (2000). https://doi.org/10.1023/A:1008742513754

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