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Variation and population structure at microsatellite and isozyme loci in wild cabbage ( Brassica oleracea L.) in Dorset (UK)

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Abstract

We examined variation at four isozyme and seven microsatellite loci in natural populations of Brassica oleracea L. on the coast of Dorset. All loci were polymorphic, and the diversity index of isozyme loci was similar to that of the microsatellites. Both microsatellites and isozymes showed significant spatial differentiation of genetic variation, but the scale differed between the marker types. Significant non-random mating at isozyme loci was detected within small groups of about five plants, in which adjacent individuals were separated by less than 5 m. On the other hand, non-random mating at microsatellite loci was only detected within the Dorset region as a whole. FST was significant for all loci and there was evidence of isolation by distance at both microsatellite and isozyme loci. From these data we infer that there is low but significant amounts of gene flow among B. oleracea populations in Dorset. The differences in the genetic structure between the two types of marker may be due to higher mutation rates at the microsatellite loci; however, the data suggest that stepwise gain or loss of single repeat units is not the principal mechanism.

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References

  • Arus, P. & T.J. Orton, 1983. Inheritance and linkage relationships of isozyme loci in Brassica oleracea. J. Hered. 74: 405–412.

    Google Scholar 

  • Carafa, A.M. & G. Carratu, 1997. Stigma treatment with saline solutions: a new method to overcome self-incompatibility in Brassica oleracea L. J. Hort. Sci. 72: 531–535.

    Google Scholar 

  • Charlesworth, D. & P. Awadalla, 1998. Flowering plant selfincompatibility: the molecular population genetics of Brassica S-loci. Heredity 81: 1–9.

    PubMed  Google Scholar 

  • Cornuet, J.M. & G. Luikart, 1996. Description and power analysis of two tests for detecting recent bottlenecks from allele frequency data. Genetics 144: 2001–2014.

    PubMed  Google Scholar 

  • Dallas, J.F., F. Bonhomme, P. Boursot, J. Britton-Davidian & V. Bauchau, 1998. Population genetic structure in a Robertsonian race of house mice: evidence from microsatellite polymorphism. Heredity 80: 70–77.

    PubMed  Google Scholar 

  • Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: version II. Pl. Mol. Biol. Rep. 1: 19–21.

    Google Scholar 

  • Di Rienzo, A., A.C. Peterson, J.C. Garza, A.M. Valdes, M. Slatkin & N.B. Freimer, 1994. Mutational processes of simple-sequence repeat loci in human populations. Proc. Natl. Acad. Sci. U.S.A. 91: 3166–3170.

    PubMed  Google Scholar 

  • Fang, G., S. Hammar & R. Grumet, 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13: 52–56.

    PubMed  Google Scholar 

  • Goudet, J., 1995. FSTAT (Version 1.2): a computer program to calculate F-statistics. J. Hered. 86: 485–486.

    Google Scholar 

  • Goudet, J., T. De Meeüs, A.J. Day & C.J. Gliddon, 1994. The different levels of population structuring of the dogwhelk, Nucella lapillus, along the south Devon coast. In: Beaumont, A.R. (Ed.), Genetics and Evolution of Aquatic Organisms, Chapman & Hall, London, pp. 81–95.

    Google Scholar 

  • Goudet, J., M. Raymond, T. De Meeüs & F. Rousset, 1996. Testing differentiation in diploid populations. Genetics 144: 1993–1940.

    Google Scholar 

  • Grandclement, C. & G. Thomas, 1996. Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor. Appl. Genet. 93: 86–90.

    Google Scholar 

  • Greenhalgh, J.R. & N.D. Mitchell, 1976. The involvement of flavour volatiles in the resistance to downy mildew of wild and cultivated forms of Brassica oleracea. New Phytol. 77: 391–398.

    Google Scholar 

  • Hamrick, J.L. & M.J.W. Godt, 1996. Effects of life-history traits on genetic diversity in plant species. Phil. Trans. R. Soc. Lond. B 351: 1291–1298.

    Google Scholar 

  • Hu, J., J. Sadowski, T.C. Osborn, B.S. Landry & C.F. Quiros, 1998. Linkage group alignment from four independent Brassica oleracea RFLP maps. Genome 41: 226–235.

    Google Scholar 

  • Hurtrez-Boussès, S., 1996. Genetic differentiation among natural populations of the rare Corsican endemic Brassica insularis Moris: implications for conservation guidelines. Biol. Conserv. 76: 25–30.

    Google Scholar 

  • Kresovich, S., A.K. Szewc-McFadden, S.M. Bliek, & J.R. McFerson, 1995. Abundance and characterisation of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed). Theor. Appl. Genet. 91: 206–211.

    Google Scholar 

  • Lannér-Herrera, C., M. Gustafsson, A.-S. Fält & T. Bryngelsson, 1996. Diversity in natural populations of wild Brassica oleracea as estimated by isozyme and RAPD analysis. Genet. Res. Crop Evol. 43: 13–23.

    Google Scholar 

  • Lydiate, D., A. Sharpe, U. Langercrantz & I. Parkin, 1993. Mapping the Brassica genome. Outl. Agric. 22: 85–92.

    Google Scholar 

  • Mantel, N., 1967. The detection of disease clustering and a generalised regression approach. Cancer Res. 27: 209–220.

    PubMed  Google Scholar 

  • Mellersh, C. & J. Sampson, 1993. Simplifying detection of microsatellite length polymorphisms. Biotechniques 15: 582.

    PubMed  Google Scholar 

  • Mitchell, N.D., 1976. The status of Brassica oleracea L. subsp. oleracea (wild cabbage) in the British Isles. Watsonia 11: 97–103.

    Google Scholar 

  • Mitchell, N.D. & A.J. Richards, 1979. Biological Flora of the British Isles No 145 Brassica oleracea L. subsp. oleracea. J. Ecol. 67: 1087–1096.

    Google Scholar 

  • Mithen, R., A.F. Raybould & A. Giamoustaris, 1995. Divergent selection for secondary metabolites between wild populations of Brassica oleracea and its implications for plant herbivore interactions. Heredity 75: 472–484.

    Google Scholar 

  • Nauta, M.J. & F.J. Weissing, 1996. Constraints on allele size at microsatellite loci: implications for genetic differentiation. Genetics 143: 1021–1032.

    PubMed  Google Scholar 

  • Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. U.S.A. 70: 3321–3323.

    PubMed  Google Scholar 

  • Nelson, A., 1927. Fertility in the genus Brassica. J. Genet. 18: 109–135.

    Google Scholar 

  • Ockendon, D.J., 1974. Distribution of self-incompatibility alleles and breeding structure of open-pollinated cultivars of Brussels sprouts. Heredity 33: 159–171.

    Google Scholar 

  • Pemberton, J.M., J. Slate, D.R. Bancroft & J.A. Barrett, 1995. Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Mol. Ecol. 4: 249–252.

    PubMed  Google Scholar 

  • Pfeiffer, A., A.M. Olivieri & M. Morgante, 1997. Identification and characterization of microsatellites in Norway spruce. Genome 40: 411–419.

    PubMed  Google Scholar 

  • Raybould, A.F., A.J. Gray, M.J. Lawrence & D.F. Marshall, 1991. The evolution of Spartina anglica (C.E. Hubbard): origin and genetic variability. Biol. J. Linn. Soc. 43: 111–126.

    Google Scholar 

  • Raybould, A.F., J. Goudet, R.J. Mogg, C.J. Gliddon & A.J. Gray, 1996. Genetic structure of a linear population of sea beet (Beta vulgaris ssp. maritima) revealed by isozyme and RFLP analysis. Heredity 76: 111–117.

    Google Scholar 

  • Raybould, A.F., R.J. Mogg, C. Aldham, C.J. Gliddon, R.J. Thorpe & R.T. Clarke, 1998. The genetic structure of sea beet (Beta vulgaris ssp. maritima) populations. III. Detection of isolation by distance at microsatellite loci. Heredity 80: 127–132.

    Google Scholar 

  • Raymond, M., 1997. Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance. Genetics 145: 1219–1228.

    PubMed  Google Scholar 

  • Raymond, M. & F. Rousset, 1995. genepop (Version 1.2): population genetics software for exact tests and ecumenicism. J. Hered. 86: 248–249.

    Google Scholar 

  • Rether, B., G. Delmas & A. Laouedj, 1993. Isolation of polysaccharide-free DNA from plants. Pl. Mol. Biol. Rep. 11: 333–337.

    Google Scholar 

  • Rousset, F. & M. Raymond, 1995. Testing heterozygote excess and deficiency. Genetics 140: 1413–1419.

    PubMed  Google Scholar 

  • Saghai Maroof, M.A., R.M. Biyashev, G.P. Yang, Q. Zhang & R.W. Allard, 1994. Extraordinary polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc. Natl. Acad. Sci. U.S.A. 91: 5466–5470.

    PubMed  Google Scholar 

  • Slatkin, M., 1985. Gene flow in natural populations. Ann. Rev. Ecol. Syst. 16: 393–430.

    Google Scholar 

  • Slatkin, M., 1993. Isolation by distance in equilibrium and nonequilibrium populations. Evolution 47: 264–279.

    Google Scholar 

  • Slatkin, M., 1995. A measure of population subdivision based on microsatellite allele frequencies. Genetics 139: 457–462.

    PubMed  Google Scholar 

  • Snogerup, S., M. Gustafsson & R. von Bothmer, 1990. Brassica sect. Brassica (Brassicaceae). Willdenowia 19: 271–365.

    Google Scholar 

  • Song, K., T.C. Osborn & P.H. Williams, 1990. Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 3. Genome relationships in Brassica and related genera and the origin of Brassica oleracea and B. rapa (syn. campestris). Theor. Appl. Genet. 79: 497–506.

    Article  Google Scholar 

  • Streiff, R., T. Labbe, R. Bacilieri, H. Steinkellner, J. Glossl & A. Kremer, 1998. Within population genetic structure in Quercus robor L. and Quercus petraea (Matt.) Liebl. assessed with isozymes and microsatellites. Mol. Ecol. 7: 317–328.

    Google Scholar 

  • Szewc-McFadden, A.K., S. Kresovich, S.M. Bliek, S.E. Mitchell & J.R. McFerson, 1996. Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species. Theor. Appl. Genet. 93: 534–538.

    Google Scholar 

  • Warman, E.A., 1999. Gene flow in wild cabbage. In: Gray, A.J., F. Amijee & C.J. Gliddon (Eds.), Environmental Impact of Genetically Modified Crops. Department of the Environment, Transport and the Regions, London (in press).

    Google Scholar 

  • Weedon, J.F. & N.F. Wendel, 1989. Visualisation and interpretation of plant isozymes. In: Soltis, D.E. & P.E. Soltis (Eds.), Isozymes in Plant Biology, Chapman & Hall, London, pp. 5–45.

    Google Scholar 

  • Weir, B.S. & C.C. Cockerham, 1984. Estimating F-statistics for the analysis of population structure. Evolution 38: 1358–1370.

    Google Scholar 

  • Wills, A.B. & E.M. Wiseman, 1980. Acid phosphatase isoenzymes of Brassica oleracea seedlings and their application to sib testing in F1 hybrids. Ann. Appl. Biol. 94: 137–142.

    Google Scholar 

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Authors and Affiliations

  1. Furzebrook Research Station, Institute of Terrestrial Ecology, Wareham, Dorset, BH20 5AS, UK Author for correspondence; Tel

    A.F. Raybould, R.J. Mogg, R.T. Clarke & A.J. Gray

  2. School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, Wales, UK

    C.J. Gliddon

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  1. A.F. Raybould
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  2. R.J. Mogg
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  3. R.T. Clarke
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  4. C.J. Gliddon
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  5. A.J. Gray
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Correspondence to A.F. Raybould.

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Raybould, A., Mogg, R., Clarke, R. et al. Variation and population structure at microsatellite and isozyme loci in wild cabbage ( Brassica oleracea L.) in Dorset (UK). Genetic Resources and Crop Evolution 46, 351–360 (1999). https://doi.org/10.1023/A:1008658630440

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