Abstract
A putative basic DNA-binding protein (BDBP) gene was identified in the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV) genome. The ORF is 330 nucleotides long encoding a basic protein of 109 amino acids with a molecular mass of 11.6 kDa. It is the first BDBP identified in single nucleocapsid NPVs and a homologue of Autographa californica MNPV (AcMNPV) P6.9. A consensus late transcription motif, ATAAG, was found at 57 nt upstream of the translational start codon and a polyadenylation signal was observed at 172 nt downstream of the stop codon. A major transcript of 620 nt was first observed in HearNPV-infected Hz2e5 cells 16 h post infection. Primer extension analysis revealed that this transcript initiated from the first residue of the consensus ATAAG late transcription start motif. Comparison with other baculoviral BDBPs showed that they all contained two conserved cAMP- and cGMP-dependent protein kinase phosphorylation motifs, R-R-R-S. The HearNPV P6.9 homologue is the longest BDBP found so far in baculoviruses.
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Fitt G.P. Ann Rev Entomol 34, 17-52, 1989.
Zhang G., Zhang Y., Ge L., and Shan Z., Acta Phytophyla Sin 8, 235-240, 1981.
Sun X. and Zhang G., Virol Sin 9, 309-318, 1994.
Chen X., Hu Z.H., and Vlak J.M., Virol Sin 12, 346-353, 1997.
Chen X., Hu Z.H., Jehle J.A., and Vlak J.M., Virus Genes 15, 219-225, 1997.
Chen X., Ijkel W.F.J., Domony C., Zanotto P.A., Hashimoto Y., Faktor O., Hayakawa T., Wang C.H., Krell P.J., Hu Z.H., and Vlak J.M., Virus Res 65, 21-32, 1999.
Funk C.J., Braunagel S.C., and Rohrmann G.F., in Miller L.K. (ed). The baculoviruses. Baculovirus structure, L.K., Plenum Press, New York and London, 1997, pp. 12-14.
Bud H.M. and Kelly D.C., J Ultrastruct Res 73, 361-368, 1980.
Tweeten K.A., Bulla L.A. Jr., and Consigli R.A., J Virol 33, 866-876, 1980.
Kelly D.C., Brown D.A., Ayres M.D., Allen C.J., and Walker I.O., J Gen Virol 64, 399-408, 1983.
Wilson M.E. and Consigli R.A., Virology 143, 526-535, 1985.
Miller L.K., Adang M.J., and Browne D., J Virol 46, 275-278, 1983.
Funk C.J. and Consigli R.A., J Virol 66, 3168-3171, 1992.
Funk C.J. and Consigli R.A., Virology 193, 396-402, 1993.
Wilson M.E., Virus Res 9, 21-23, 1988.
Russell R.L.Q. and Rohrmann G.F., J Gen Virol 71, 551-560, 1990.
Maeda S., Kamita S.G., and Kataoka H., Virology 180, 807-810, 1991.
Jehle J.A. and Backhaus H., J Gen Virol 75, 1815-1820, 1994.
Kuzio J., Pearson M.N., Harwood S.H., Funk C.J., Evans J.T., Slavicek J.M., and Rohrmann G.F., Virology 253, 17-34, 1999.
Hayakawa T.K.R., Okano K., Seong S.I., Goto C., and Maeda S., Virology 262, 277-297, 1999.
IJkel W.F.J., Van Strien E.A., Heldens J.G.M., Broer R., Zuidema D., Goldbach R.W., and Vlak J.M., J Gen Virol 80, 3289-3304, 1999.
Lu A., Seshagiri S., and Miller L.K., Biol Control 7, 320-332, 1996.
O'Reilly D.K., Miller L.K., and Luckow V.A., Baculovirus expression vectors. A laboratory manual, W.H. Freeman and Company, New York, 1992, pp. 132.
Sambrook E.M., Fritsch E.F., and Maniatis T., Molecular Cloning. A laboratory guide, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.
Sanger F., Nichlen S., and Coulson A.R., Proc Natl Acad Sci USA 74, 5463-5467, 1977.
Altschul S.F., Gish W., Miller W., Meyers E.W., and Lipman D.J., J Mol Biol 215, 403-410, 1992.
Bairoch A., Bucher P., and Hofmann K., Nucl Acid Res 25, 217-221, 1997.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G., Nucl Acids Res 25, 4876-4882, 1997.
Chomczynski P. and Sacchi N., Biochemistry 162, 156-159, 1987.
Ausubel F., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K., Current protocols in molecular biology, Vol. 3. John Wiley and Sons, Inc., 1996.
Church G.M. and Gilbert W., Proc Natl Acad Sci USA 81, 1991-1995, 1984.
Lu A. and Carstens E.B., Virology 181, 336-347, 1991.
Heldens J.G., Liu Y., Zuidema D., Goldbach R.W., and Vlak J.M., Virus Res 55, 187-198, 1998.
Wilson M.E., Mainprize T.H., Friesen P.D., and Miller L.K., J Virol 61, 661-666, 1987.
Rivkin H., Mor M., and Chejanovsky N., Virus genes 17, 11-19, 1998.
Feramisco J.R., Glass D.B., and Krebs E.G., J Biol Chem 255, 4240-4245, 1980.
Glass D.B. and Smith S.B., J Biol Chem 258, 14797-14803, 1983.
Kishimoto A., Nishizuka K., Nakanishi H., Uratsuji Y., Nomura H., Takeyama Y., and Nishizuka Y., J Biol Chem 260, 12492-12499, 1985.
Balhorn R., J Cell Biol 93, 298-305, 1982.
Stryer L., Biochemistry, Fourth edition W.H. Freeman and Company, New York, 1995.
Bonning B.C., Roelvink P.W., Vlak J.M., Possee R.D., and Hammock B.D., J Gen Virol 75, 1551-1556, 1994, pp. 421.
Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., and Possee R.D., Virology 202, 586-605, 1994.
Ahrens C.H., Russell R.L., Funk C.J., Evans J.T., Harwood S.H., and Rohrmann G.F., Virology 229, 381-399, 1997.
Kamita S.G. and Maeda S., Gene 190, 173-179, 1997.
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Wang, H., Chen, X., Wang, H. et al. Nucleotide Sequence and Transcriptional Analysis of a Putative Basic DNA-Binding Protein of Helicoverpa armigera Nucleopolyhedrovirus. Virus Genes 22, 113–120 (2001). https://doi.org/10.1023/A:1008194605429
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DOI: https://doi.org/10.1023/A:1008194605429