Abstract
The polyhedrin gene (polh) of a characteristically distinct Spodoptera litura nuclear polyhedrosis virus isolate (SIMNPV) is identified in the HindIII-F fragment of the viral DNA. The nucleotide sequence of the 1057 base pair (bp) region of this fragment contains an open reading frame (ORF) without any intervening sequence for coding a polypeptide of 246 amino acids. Analysis of the nucleotide sequence and deduced amino acid sequence indicate that this has more than 70% sequence identity to known polyhedrins. The coding region is preceded by an AT rich region containing the conserved late promoter motif TAAG. The upstream promoter and coding regions of this polh gene are more similar to polh of the NPVs of Spodoptera frugiperda (Sf), Spodoptera exigua (Se) and Panolis flamea (Pf).
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Blissard G.W. and Rohrmann G.F., Ann Rev Entomol 53, 127–155, 1990.
Rohrmann G.F., J Gen Virol 73, 749–761, 1992.
Mathews R.E.F., Intervirology 17, 1–199, 1982.
Francki R.I.B., Faquet C.M., Knudson D.L., and Brown F., Arch Virol 2, (Suppl.), 119, 1991.
Tinsley T.W. and Kelley D.C., in Maramorosch K. and Sherman K.E. (eds). Viral Insecticides for Biological Control. Academic Press, New York, 1985, p. 7.
Falcon L.A. and Hess R.T., J Invert Pathol 45, 356–359, 1985.
Cowan P., Bulach D., Goodge K., Robertson A., and Tribe D.E., J Gen Virol 75, 3211–3218, 1994.
Zanatto P.M.A., Kessing B.D., and Maruniak J.E., J Invert Pathol 62, 147–164, 1993.
Smith G.E. and Summers M.D., Virology 123, 393–406, 1982.
Summers M.D., Smith G.E., Knell J.D., and Burand J.P., J Virol 34, 696–703, 1980.
Croizier G. and Quiot J.M., Ann Virol 132, 3–18, 1981.
Kondo A. and Maeda S., J Virol 65, 3625–3632, 1991.
Granados R.R. and Williams K.A., in Fredrici B. and Granados R. (eds). The Biology of Baculoviruses I. CRC Press, Boca Raton, FL., 1986, pp. 89–108.
Rohrmann G.F., Pearson M.N., Bailey T.J., Becker R.R., and Beauderau G.S., J Mol Evol 17, 329–333, 1981.
Das R.H. and Prasad Y.D., Biochem Mol Biol Intl 39, 1–11, 1996.
Ignoffo C.M., J Invert Pathol 7, 217–226, 1965.
Das R.H., Bansal O.B., Behera A.K., Durgaprasad Y., Kumar M., and Bali A., Biotechniques 20, 364–368, 1996.
McIntosh A.H. and Ignoffo C.M., J Invert Pathol 41, 27–32, 1983.
Bansal O.B. and Das R.H., Nucl Acids Res 17, 10129, 1989.
Sambrook J., Fritsch E.F., and Maniatis T. (eds). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York, 1989.
Danna K.J., in Grossman L. and Moldave K. (eds). Methods in Enzymology 65. Academic Press, New York, 1988, pp. 449–494.
Southern E.M., Anal Biochem 100, 319–323, 1979.
Southern E.M., J Mol Biol 98, 503–508, 1975.
Sanger F., Nickler S., and Coulson A.R., Proc Natl Acad Sci USA 74, 5463–5467, 1977.
Croizier L. and Croizier G., Biochim Biophys Acta 1218, 457–459, 1994.
Matsura Y., Possee R.D., Overtone H.A., and Bishop D.H.L., J Gen Virol 68, 1233–1250, 1987.
Van Strein E.A., Zuidema D., Goldbach R.W., and Vlak J.M., J Gen Virol 73, 2813–2821, 1992.
Morris T.D. and Miller L.K., Gene 140, 147–153, 1994.
Behera A.K., Kumar M., Bansal A., Bansal O.B., and Das R.H., Gene 190, 145–150, 1997.
Cameron I.R. and Possee R.D., Virus Res 12, 183–199, 1989.
Gonzalez M.A., Smith G.E., and Summers M.D., Virology 170, 160–175, 1989.
Possee R.D. and Kelly D.C., J Gen Virol 69, 1285–1298, 1988.
Oakey R., Cameron I.R., Davis B., Davis E., and Possee, R.D., J Gen Virol 70, 769–775, 1989.
Iddekinge Hooft Van B.J.L., Smith G.E., and Summers M.D., Virology 131, 561–565, 1983.
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Bansal, O.B., Bansal, A., Behera, A.K. et al. Characterization of the Polyhedrin Gene of Spodoptera litura Nuclear Polyhedrosis Virus. Virus Genes 14, 175–180 (1997). https://doi.org/10.1023/A:1007975608494
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DOI: https://doi.org/10.1023/A:1007975608494