Abstract
Partial outlet obstruction of the rabbit urinary bladder causes increased tissue hypertrophy and decreased contractility of that organ; we showed that, in an experimental rabbit model, both correlate closely with alterations in the status and expression of mitochondrial (mt), and mt-related nuclear, genetic parameters in bladder smooth muscle. Here we investigate the rate and overall level of recovery of mt and nuclear genetic function following reversal of outlet obstruction in the same animal model. Release from outlet obstruction at 28 days resulted in improvement in both level of hypertrophy and contractile function in all bladders studied. However, bladders fell into two groups based on whether relative copy mt genome number per cell was above or below that of unobstructed controls. Bladders with high mt DNA content adjusted organellar genome copy number toward normal post-reversal but did not properly adjust mt transcript levels; mt-related nuclear transcripts in these samples showed recovery. Bladders with low mt DNA content showed no adjustment of those levels toward normal post-reversal but did show some adjustment in other mt and nuclear genetic parameters. Thus, a limiting factor for return of normal bladder function following reversal of outlet obstruction may be recovery of normal mt genetic performance.
Similar content being viewed by others
References
Sterling AM, Ritter RC, Zinner NR: The physical basis of obstructive uropathology. In: F Hinman (ed). Benign Prostafic Hypertrophy. Springer-Verlag, New York, 1983. pp 433–442
Levin RM, Longhurst PA, Monson FC, Haugaard N, Wein AJ: Experimental studies on bladder outlet obstruction. In: H Lepor, RK Lawson (eds). Prostate Diseases. WB Saunders, Philadelphia, 1993. pp 119–130
Ghoniem GM, Regnier CH, Biancani P, Johnson L, Susset JG: Effect of vesical outlet obstruction on detrusor contractility and passive properties in rabbits. J Urol 135: 1284–1289, 1986
Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ: Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. Prostate (Supp 3): 9–26, 1990
Bilgen A, Wein AJ, Haugaard B, Packard D, Levin RM: Effect of outlet obstruction on pyruvate metabolism of the rabbit urinary bladder. Mol Cell Biochem 117: 159–163, 1992
Haugaard N, Potter L, Wein AJ, Levin RM: Effect of partial outlet obstruction of the rabbit urinary bladder on malate dehydrogenase and citrate synthase activity. J Urol 147: 1391–1393, 1992
Levin RM, Haugaard N, Levin SS, Wein AJ: Creatine kinase activity in normal and hypertrophied rabbit urinary bladder tissue (following partial outlet obstruction). Mol Cell Biochem 106: 143–149, 1991
Buttyan R, Jacobs B, Blaivis JG, Levin RM: The early molecular response to rabbit bladder outlet obstruction. Neurourol Urodynam 11: 253–260, 1992
Zhao Y, Chacko S, Levin RM: Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction. Mol Cell Biochem 130: 49–55, 1994
Zhao Y, Levin RM, Levin SS, Nevel CA, Haugaard N, Hueih-Shing Hsu T, Hudson AP: Partial outlet obstruction of the rabbit bladder results in changes in the mitochondrial genetic system. Mol Cell Biochem 141: 47–55, 1994
Nevel-McGarvey CA, Levin RM, Hudson AP: Transcription of mitochondrial and mitochondria-related nuclear genes in rabbit bladder following partial outlet obstruction. Mol Cell Biochem 173: 95–102, 1997
Nagley P: Coordination of gene expression in the formation of mammalian mitochondria. Trends Genet 7: 1–4, 1991
Levin RM, Malkowicz SB, Wein AJ, Atta MA, Elbadawi A: Recovery from short term obstruction of the rabbit urinary bladder. J Urol 134: 388–390, 1985
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989
Horikoshi T, Danenberg K, Volkenandt M, Stadibauer T, Danenberg PV: Quantitative measurement of relative gene expression in human tumors. In: B.A. White (ed). Methods in Molecular Biology, Vol 15; PCR Protocols: Current Methods and Applications. Humana Press lnc, Totowa, NJ, 1993. pp 177–188
McDaniel T, Meltzer SJ: Direct radioactive labeling of polymerase chain reaction products. In: BA White (ed). Methods in Molecular Biology, Vol 15; PCR Protocols: Current Methods and Applications. Humana Press, Totowa, NJ, 1993. p 41–43
Gross-Bellard M, Oudet P, Chambon P: Isolation of high molecular weight DNA from mammalian cells. Eur J Biochem 36: 32–38, 1972
Robinson JB, Breut LG, Sumegi B, Srere PA: An enzymatic approach to the study of the Krebs tricarboxylic acid cycle. In: VM Darley-Usmar, D Rickwood, MT Wilson (eds). Mitochondria: A Practical Approach. IRL Press, Oxford, 1989. pp 137–145
Daley-Usmar VM, Capaldi RA, Takamiya S, Millet F, Wilson MT, Malatesta F, Sarti P: Reconstitution and molecular analysis of the respiratory chain. In: V.M. Darley-Usmar, D. Rickwood, M.T. Wilson (eds). Mitochondria: A Practical Approach. IRL Press, Oxford, 1989. pp. 137–145
Lowry OM, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 268–271, 1951
Jeon KW, Friediander M: Mitochondrial Genomes. lnt Rev Cytol, Vol 141. Academic Press, NY, 1992
Monson FC, Wein AJ, Eika B, Murphy M, Levin RM: Stimulation of DNA synthesis in rabbit bladder wall after partial obstruction and acute overdistension. Neurourol Urodynam 13: 51–62, 1994
Clayton D: Replication and transcription of the mammalian mitochondrial genome. Annu Rev Biochem 53: 575–594, 1991
Ropp PA, Copeland WC: Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase γ. Genomics 36: 449–458, 1996
Shadel GS, Clayton DA: Mitochondrial DNA maintenance in vertebrates. Annu Rev Biochem 66: 409–435, 1997
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nevel-McGarvey, C.A., Rohrmann, D., Levin, R.M. et al. Mitochondrial and mitochondia-related nuclear genetic function in rabbit urinary bladder following reversal of outlet obstruction. Mol Cell Biochem 197, 161–172 (1999). https://doi.org/10.1023/A:1006945732718
Issue Date:
DOI: https://doi.org/10.1023/A:1006945732718