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Gelling agent influences the detrimental effect of kanamycin on adventitious budding in flax

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Abstract

Flax (Linum usitatissimum L.) hypocotyls were cultivated on regeneration media containing various concentrations of kanamycin (an aminoglycoside antibiotic commonly used to select transgenic plant material) solidified with three different gelling agents: gellan gum, agar and a mixture of both. The inhibitory effect of kanamycin on bud regeneration was analyzed. A significant interaction was observed between the nature of the gelling agent and the kanamycin concentration. The antibiotic concentration needed to strongly inhibit bud production varied greatly with the nature of the gelling agent. Gellan gum lowered the inhibitory effect of kanamycin.

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Authors and Affiliations

  1. Laboratoire de Biotechnologie et Physiologie Végétales, UFR Sciences, 33 rue St Leu, 80039, Amiens cedex, France

    E. Laine, E. Laine, F. Lamblin, J. Lacoux, P. Dupre, D. Roger & A. David

  2. Laboratoire de Morphogenèse Végétale Expérimentale, Université Paris Sud, Bat 360, 91405, Orsay cedex, France

    D. Sihachakr

Authors
  1. E. Laine
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  2. F. Lamblin
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  3. J. Lacoux
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  4. P. Dupre
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  5. D. Roger
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  6. D. Sihachakr
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  7. A. David
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Correspondence to E. Laine.

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Laine, E., Lamblin, F., Lacoux, J. et al. Gelling agent influences the detrimental effect of kanamycin on adventitious budding in flax. Plant Cell, Tissue and Organ Culture 63, 77–80 (2000). https://doi.org/10.1023/A:1006455918041

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  • DOI: https://doi.org/10.1023/A:1006455918041

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