Abstract
Immature zygotic embryos of durum wheat cv ‘Ardente’ were cultured vitro on 2,4-D to induce somatic embryogenesis. Five days after culture initiation, somatic proembryos were directly initiated from the scutellum of immature embryos. After 28 days, somatic embryos were fully developed with a scutellum-like structure. Histological observations between the first and the eighty day in culture showed a clear unicelllar origin for a few of these somatic embryos, whilst most of them originated from a meristematic multilayer. Furthermore, estimation of the mitotic index of outer epithelium, subepithelium and inner epithelium of the scutellum during the first week of culture, showed a strict epidermal origin of these early developed structures. The addition of 1 mg·L−1 of AgNO3 enhanced the induction of direct somatic embryogenesis (a more than 22 fold increase), affecting both the percentage of embryogenic explants and the number of somatic embryos per explant, suggesting the possible involvement of ethylene.
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References
Ahmed KZ and Sagi F (1993) Culture of and fertile plant regeneration from regenerable embryogenic suspension cell-derived protoplasts of wheat (Triticum aestivum L.). Plant Cell Rep 12: 175–179
Auboiron E, Carron MP and Michaux-Ferrière N (1990) Influence of atmospheric gases, particularly ethylene on somatic embryogenesis of Hevea brasiliensis. Plant Cell Tissue Organ Cult 21: 31–37
Barcelo P, Lazzeri PA, Hernandez P, Martin A and Lörz H (1993) Morphogenic cell and protoplast culture of Trithordeum. Plant Sci 88: 209–218
Bennici A, Caffaro L, Dameri RM, Gastaldo P and Profumo P (1988) Callus formation and plantlet regeneration from immature Triticum durum Desf. embryos. Euphytica 39: 255–263
Bhaskaran S and Smith RH (1990) Regeneration in cereal tissue culture. Crop Sci 30: 1328–1336
Bommineni VR and Jauhar PP (1996) Regeneration of plantlets through isolated scutellum culture of durum wheat. Plant Sci 116: 197–203
Borelli GM, Lupotto E, Locatelli F and Wittmer G (1991) Long-term embryogenic cultures in durum wheat (Triticum durum Desf.). Plant Cell Rep 10: 296–299
Chang YF, Wang WC, Warfield CY, Nguyen HT and Wong JR (1991) Plant regeneration from protoplasts isolated from longterm cell cultures of wheat (Triticum aestivum L.). Plant Cell Rep 9: 611–614
Chowdhury MKU, Vasil V and Vasil IK (1994) Molecular analysis of plants regenerated from embryogenic cultures of wheat. Theor Appl Genet 87: 821–824
Cho UH and Kasha KJ (1989) Ethylene production and embryogenesis from anther cultures of barley (Hordeum vulgare). Plant Cell Rep 8: 415–417
Eapen S and Rao PS (1982) Plant regeneration from callus cultures of durum and emmer wheat. Plant Cell Rep 1: 215–218
Emons AMC (1994) Somatic embryogenesis: cell biological aspects. Acta Bot Neerl 43: 1–14
Felföldi K and Purnhauser L (1992) Induction of regenerating callus cultures from immature embryos of 44 wheat and 3 triticale cultivars. Cereal Res Comm 20: 273–277
Fisher DB (1968) Protein staining of ribboned epon sections for light microscopy. Histochemie 16: 92–96
Fujimura T and Komamine A (1980) The serial observation of embryogenesis in a carrot cell suspension culture. New Phytol 86: 213–218
Galiba G and Yamada Y (1988) A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation. Plant Cell Rep 7: 55–58
Greco B, Tanzarella OA and Blanco A (1984) Plant regeneration from leaf base callus in durum wheat (Triticum durum Desf.). Cereal Res Comm 12: 171–177
He DG, Tanner G and Scott KJ (1986) Somatic embryogenesis and morphogenesis in callus derived from the epiblast of immature embryos of (Triticum aestivum L.). Plant Sci 45: 119–124
Jones TJ and Rost TL (1989) The developmental anatomy and ultrastructure of somatic embryos of rice (Oryza sativa L.) scutellum epithelial cells. Bot Gaz 150: 41–49
Magnusson I and Bornman CH (1985) Anatomical observations on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum. Physiol Plant 63: 137–145
Maheshwari N, Rajyalakshmi K, Baweja K, Dhir SK, Chowdhuy CN and Maheshwari SC (1995) In vitro culture of wheat and genetic transformation: retrospect and prospect. Crit Rev Plant Sci 14: 149–178
Martoja R and Martoja M (1967) Initiation aux techniques de l'histologie animale. Masson et Cie, Paris
McCain JW and Hodges TK (1986) Anatomy of somatic embryos from maize embryo culture. Bot Gaz 147: 453–460
Mohmand AS and Nabors MW (1991) Comparison of two methods for callus culture and plant regeneration in wheat (Triticum aestivum L.). Plant Cell Tissue Organ Cult 26: 185–187
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco culture. Physiol Plant 15: 473–497
Nissen P (1994) Stimulation of somatic embryogenesis by ethylene: Effects of modulators of ethylene biosynthesis and action. Physiol Plant 92: 397–403
Oka S, Saito N and Kawaguchi H (1995) Histological observations on initiation and morphogenesis in immature and mature embryo derived callus of barley (Hordeum vulgare L.). Ann Bot 76: 487–492
Ozias-Akins P and Vasil K (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): evidence for somatic embryogenesis. Protoplasma 110: 95–105
Redway FA, Vasil V, Lu D and Vasil IK (1990) Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Theor Appl Genet 79: 609–617
Ryschka S, Ryschka U and Schulze J (1991) Anatomical studies on the development of somatic embryoids in wheat and barley explants. Biochem Physiol Pflanzen 187: 31–41
Sévenier R and Coumans M (1996) Ethylene production and involvement during the first steps of durum wheat (Triticum durum) anther culture. Physiol Plant 96: 146–151
Sharma GC, Wang WC and Sapra VT (1985) Effect of genotype, media and temperature pretreatment on callus initiation in triticale, wheat and rye anther culture. Cereal Res Commun 10: 143–150
Skirvin RM, McPheeters KD and Norton M (1994) Sources and frequency of somaclonal variation. Hort Sci 29: 1231–1237
Songstad DD, Armstrong CL and Petersen WL (1991) Silver nitrate increases type II callus production from immature embryos of maize inbred B73 and its derivatives. Plant Cell Rep 9: 699–702
Takumi S and Shimada T (1996) Production of transgenic wheat through particle bombardment of scutellar tissues: frequency is influenced by culture duration. J Plant Physiol 149: 418–423
Taylor MG and Vasil IK (1996) The ultrastructure of somatic embryo development in pearl millet (Pennisetum glaucum; Poaceae). Amer J Bot 83: 28–44
Vain P, Flament P and Soudain P (1989) Role of ethylene in embryogenic callus initiation and regeneration in Zea mays L. J Plant Physiol 537–540
Vasil V and Vasil IK (1982) The ontogeny of somatic embryos of Pennisetum americanum (L.) K. Schum. I. In cultured immature embryos. Bot Gaz 143: 454–465
Yang YM, He DG and Scott KJ (1993) Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. D6962). Plant Cell Rep 12: 320–323
Yang YM, He DG and Scott KJ (1994) Cell aggregates in wheat suspension cultures and their effects on isolation and culture of protoplasts. Plant Cell Rep 13: 176–179
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Fernandez, S., Michaux-Ferrière, N. & Coumans, M. The embryogenic response of immature embryo cultures of durum wheat (Triticum durum Desf.): histology and improvement by AgNO3. Plant Growth Regulation 28, 147–155 (1999). https://doi.org/10.1023/A:1006142504577
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DOI: https://doi.org/10.1023/A:1006142504577