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Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity

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Abstract

Apple shoots were grown in a Murashige and Skoog liquid proliferation medium containing 4.4 10 -6 M BA, and supplemented with various fractions of agar. Hydrolysed agar from Difco was able to overcome hyperhydricity when its concentration was increased to 0.7%. Among the fractions isolated from this hydrolysed agar, only oligosaccharides were found to reduce the occurrence of this developmental abnormality. The most anti-hyperhydric fraction had a molecular weight slightly less than 1900 daltons and contained methylated and sulphated galactose derivatives.

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire Végétale et Valorisation des Espèces Ligneuses – Faculté des Sciences –, 123 rue Albert Thomas –, 87060, Limoges, France

    Francoise Marga, Laurence Vebret & Henri Morvan

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  1. Francoise Marga
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  2. Laurence Vebret
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  3. Henri Morvan
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Marga, F., Vebret, L. & Morvan, H. Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity. Plant Cell, Tissue and Organ Culture 49, 1–5 (1997). https://doi.org/10.1023/A:1005832115954

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  • DOI: https://doi.org/10.1023/A:1005832115954

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