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The effect of B chromosomes and T-DNA on chromosomal variation in callus cells and regenerated roots of Crepis capillaris

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Abstract

The chromosome behaviour has been compared in three Crepis capillaris callus culture lines and the roots regenerated from these calli. The calli were obtained from explants derived from plants without and with two B chromosomes and the hairy roots were obtained from plants transformed with Agrobacterium rhizogenes. Cytological studies demonstrated that the presence of additional DNA as B chromosomes or as T-DNA had an influence on the numerical and structural variability of the standard chromosome in long-term callus cultures and in regenerated organs. The callus with two B chromosomes displayed higher levels of polyploidyzation than callus without B chromosomes. The roots regenerated from both these calli were only diploid, while roots regenerated from transformed callus were also polyploid.

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Authors and Affiliations

  1. Department of Plant Anatomy and Cytology, Silesian University, Katowice, Poland

    Jolanta Maluszynska

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  1. Jolanta Maluszynska
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Maluszynska, J. The effect of B chromosomes and T-DNA on chromosomal variation in callus cells and regenerated roots of Crepis capillaris. Plant Cell, Tissue and Organ Culture 50, 113–118 (1997). https://doi.org/10.1023/A:1005727529303

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  • DOI: https://doi.org/10.1023/A:1005727529303

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