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The structure of the avian fast skeletal muscle troponin T gene: seven novel tandem-arranged exons in the exon x region

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Abstract

To elucidate the mechanism that produces enormous molecular diversity in troponin T (TnT) of fast skeletal muscle, we determined the 5-half genomic sequence of the chicken fast muscle TnT gene. The sequence of ca. 16 kb included seven exons (exons 1, 2, 3, 4, w, 5, and 6), which have been reported previously and presumed by sequencing TnT cDNAs. Additionally we found six 15 nt and one 18 nt sequences in the region between exons 5 and 6 (i.e. the exon x region). They were encompassed by consensus splice donor and acceptor sites and preceded by putative branch sites, and designated herein as exons xa to xg. Our result shows that the sequence derived from exons x1, x2, and x3, the exons presumed previously by cDNA sequencing, is actually encoded by the seven exons xa to xg, establishing the precise gene structure in the exon x region. Based on our data, together with that on the 3-half genomic sequence of the quail fast muscle TnT gene, we conclude that the avian fast skeletal muscle TnT gene includes 27 exons, 16 of which are alternatively spliced.

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References

  • Breitbart RE and Nadal-Ginard B (1986) Complete nucleotide sequence of the fast skeletal troponin T gene. J Mol Biol 133: 313–324.

    Google Scholar 

  • Bucher EA, De la Brousse FC and Emerson CP Jr (1989) Developmental and muscle-specific regulation of avian fast skeletal troponin T isoform expression by mRNA splicing. J Biol Chem 264: 12482–12491.

    Google Scholar 

  • Ebashi S, Kodama A and Ebashi F (1968) Troponin. J Biochem 64: 465–477.

    Google Scholar 

  • Greaser ML and Gergely J (1973) Purification and properties of the components from troponin. J Biol Chem 248: 2125–2133.

    Google Scholar 

  • Jin J-P and Smillie LB (1994) An unusual metal-binding cluster found exclusively in the avian breast muscle troponin T of Galliformes and Craciformes. FEBS Lett 341: 135–140.

    Google Scholar 

  • Kimura F, Nakada K, Yonemura I, Hirabayashi T and Miyazaki J-I (1999) Tissue-specific distribution of breast-muscle-type and leg-muscle-type troponin T isoforms in birds. Biochem Biophys Acta, 1426: 505–512.

    Google Scholar 

  • Morgan MJ, Earnshaw JC and Dhoot GK (1993) Novel developmentally regulated exon identified in the rat fast skeletal muscle troponin T gene. J Cell Sci 106: 903–908.

    Google Scholar 

  • Ohshima Y and Gotoh Y (1987) Signals for the selection of a splice site in pre-mRNA. J Mol Biol 195: 247–259.

    Google Scholar 

  • Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Schachat F, Schmidt JM, Maready M and Briggs MM (1995) Chicken perinatal troponin Ts are generated by a combination of novel and phylogenetically conserved alternative splicing pathways. Dev Biol 171: 233–239.

    Google Scholar 

  • Smillie LB, Golosinska K and Reinach FC (1988) Sequences of complete cDNAs encoding four variants of chicken skeletal muscle troponin T. J Biol Chem 263: 18816–18820.

    Google Scholar 

  • Watanabe T, Takemasa T, Yonemura I and Hirabayashi T (1997) Regulation of troponin T gene expression in chicken fast skeletal muscle: involvement of an M-CAT-like element distinct from the standard M-CAT. J Biochem 121: 212–218.

    Google Scholar 

  • Yanisch-Perron C, Vieira J and Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119.

    Google Scholar 

  • Yao Y, Nakamura M, Miyazaki J-I, Kirinoki M and Hirabayashi T (1992) Expression pattern of skeletal muscle troponin T isoforms is fixed in cell lineage. Dev Biol 151: 531–540.

    Google Scholar 

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Miyazaki, JI., Jozaki, M., Nakatani, N. et al. The structure of the avian fast skeletal muscle troponin T gene: seven novel tandem-arranged exons in the exon x region. J Muscle Res Cell Motil 20, 655–660 (1999). https://doi.org/10.1023/A:1005504018059

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  • DOI: https://doi.org/10.1023/A:1005504018059

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