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Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. BG-11

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Abstract

An alkalophilic, chitinase-producing Bacillus sp. BG-11 was isolated which produced an extracellular chitinase and which was purified 16.5-fold, using standard purification techniques. The purified chitinase exhibited a broad pH and temperature optima of 7.5-9.0 and 45 deg C-55 deg C, respectively. The chitinase was stable between pH 6.0-9.0 and 50°C for more than 2 h. Half lives of enzyme at 60 deg C, 70 deg C and 80 deg C were 90 min, 30 min and 20 min respectively. Km value was 12 mg chitin per ml. Shelf life was 60 days at 4°C. Ca2+, Ni2+ and Triton-X-100 stimulated the activity up to 20% whereas Ag+, Hg2+, dithiothreitol, β-mercaptoethanol, glutathione, iodoacetic acid and iodoacetamide inhibited the activity up to 50%.

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, Chandigarh, India

    Bharat Bhushan & G.S. Hoondal

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  1. Bharat Bhushan
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  2. G.S. Hoondal
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Bhushan, B., Hoondal, G. Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. BG-11. Biotechnology Letters 20, 157–159 (1998). https://doi.org/10.1023/A:1005328508227

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