biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:637-639, 1997 | DOI: 10.1023/A:1000947319165

A method of DNA isolation from epiphytic CAM ferns for use in random amplified polymorphic DNA analysis

S.H. Lim1, L.K.C. Looi1, B.L. Ong1, Y.C. Wee1
1 School of Biological Sciences, National University of Singapore, Singapore

A method for DNA extraction from Pyrrosia piloselloides (L.) Price and Pyrrosia longifolia (Burm.) Morton utilising hexadecyltrimethylammonium bromide is described. The genomic DNA isolated was found to be relatively pure and shown to be suitable for RAPD analysis.

Keywords: genomic DNA; Pyrrosia longifolia; Pyrrosia piloselloides
Subjects: crassulacean acid metabolism; DNA, isolation for RAPD; genomic DNA; Pyrrosia longifolia; Pyrrosia piloselloides

Prepublished online: May 1, 1997; Published: December 1, 1997  Show citation

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Lim, S.H., Looi, L.K.C., Ong, B.L., & Wee, Y.C. (1997). A method of DNA isolation from epiphytic CAM ferns for use in random amplified polymorphic DNA analysis. Biologia plantarum, 39(4), 637-639. doi: 10.1023/A:1000947319165
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