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Genome Organization and Expression of the Penicillium stoloniferum Virus S

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Abstract

The complete sequences of two double-stranded RNAs (dsRNAs) (referred to S1 and S2) of Penicillium stoloniferum virus S (PsV-S) were established. The S1 dsRNA was 1,690 bp in length, and it contained a unique open reading frame (ORF) of 539 amino acids (molecular weight of 62 kDa, referred to P62). The S2 dsRNA was 1,523 bp in length, and also it contained one ORF of 434 amino acids (molecular weight of 47 kDa, referred to P47). Both S1 and S2 ORFs were identified only on the positive strand of each dsRNA segment. A sequence motif of (5′-CUG-3′) was found at the 3′-termini of the positive strands of PsV-S1 and S2 dsRNAs. The predicted amino acid sequences of S1 dsRNA showed high sequence homology with the putative RNA-dependent RNA polymerases of RNA viruses. Near full-length and positive-sense single-stranded RNAs derived from the S1 and S2 dsRNAs were detected from the PsV-infected host cell. The expressed proteins of P62 and P47 showed a positive reaction against PsV-S antiserum in Western blot analysis. Phylogenetic analysis using the RDRP sequences and the capsid proteins of the various partitiviruses revealed that PsV-S is a definite member of the partitivirus, the family Partitiviridae, and especially clusters well along with D. destructiva virus 1 and 2.

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References

  1. Buck K.W. and Kempson-Jones G.F., Nature 225, 504-505, 1970.

    Google Scholar 

  2. Buck K.W. and Kempson-Jones G.F., J Gen Virol 18, 223-235, 1973.

    Google Scholar 

  3. Bozarth R.F., Wood H.A., and Mandelbrot A., Virology 45, 516-523, 1971.

    Google Scholar 

  4. Lhoas P., Nature 230, 248-249, 1971.

    Google Scholar 

  5. Lhoas P., J Gen Virol 13, 365-367, 1971.

    Google Scholar 

  6. Still P.E., Detroy R.W., and Hesseltine C.W., J Gen Virol 27, 275-281, 1975.

    Google Scholar 

  7. Bozarth R.F. and Harley E.H., Biochim Biophys Acta 432, 329-335, 1976.

    Google Scholar 

  8. Kim J.W. and Bozarth R.F., J Gen Virol 66, 1991-2002, 1985.

    Google Scholar 

  9. Bozarth R.F., Wood H.A., and Goenaga A., Phytopathology 62, 493, 1972.

    Google Scholar 

  10. Diener T.D. and Schneider T.R., Arch Biochem Biophys 124, 401-412, 1968.

    Google Scholar 

  11. Maniatis T., Fritsch E.F., and Sambrook, J., Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1982.

    Google Scholar 

  12. Wallace D.M., in Burger S.L., and Kimmel A.R. (eds), Methods in Enzymology Vol. 152, Guide to Molecular Cloning Techniques, Academic Press, Orlando, 1982, pp. 41-48.

    Google Scholar 

  13. Bailey J.M. and Davidson N., Anal Biochem 70, 75-85, 1976.

    Google Scholar 

  14. Attoui H., Billoir F., Cantaloube J.F., Biagini P., Micco P., and Lamballerie X., J Virol Methods 89, 147-158, 2000.

    Google Scholar 

  15. Frohman M.A., in Innis M.A., Gelfand D.H., Sninsky J.J., and White T.J. (eds), PCR Protocols: A Guide to Methods and Applications, Academic Press, San Diego, 1990, pp. 28-38.

    Google Scholar 

  16. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403-410,.

  17. Higgins D., Thompson J., Gibson T., Thompson J.D., Higgins D.G., and Gibson T.J., Nucleic Acids Res 22, 4673-4680, 1994.

    Google Scholar 

  18. Osaki H., Wei C.Z., Arakawa M., Iwanami T., Nomura K., Matsumoto N., and Ohtsu Y., Virus Genes 25(1), 101-107, 2002.

    Google Scholar 

  19. Poch O., Sauvaget I., Delarue M., and Tordo N., EMBO J 8, 3867-3874, 1989.

    Google Scholar 

  20. Nogawa M., Kageyama T., Nakatani A., Taguchi G., Shimosaka M., and Okazaki M., Biosci Biotechnol Biochem 60, 784-788, 1996.

    Google Scholar 

  21. Yao J.M., McElreath S.D., and Tainter F.H., Curr Microbiol 34, 67-69, 1997.

    Google Scholar 

  22. Rong R., Rao S., Scott S.W., and Tainter F.H., Curr Microbiol 42, 144-148, 2001.

    Google Scholar 

  23. Compel P., Papp I., Bibo M., Fekete C., and Hornok L., Virus Genes 18(1), 49-56, 1999.

    Google Scholar 

  24. Oh C.S. and Hillman B.I., J Gen Virol 76(Pt 6), 1461-1470, 1995.

    Google Scholar 

  25. Strauss E.E., Lakshman D.K., and Tavantzis S.M., J Gen Virol 81(Pt 2), 549-555, 2000.

    Google Scholar 

  26. Buck K.W. and Kempson-Jones G.F., J Gen Virol 22, 441-445. 1974.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, Inha University, Incheon, 402-751, Korea

    Jong Wook Kim & Kyung Mi Kim

  2. Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea

    Shi Youn Kim

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  1. Jong Wook Kim
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  2. Shi Youn Kim
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  3. Kyung Mi Kim
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Correspondence to Jong Wook Kim.

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Kim, J.W., Kim, S.Y. & Kim, K.M. Genome Organization and Expression of the Penicillium stoloniferum Virus S. Virus Genes 27, 249–256 (2003). https://doi.org/10.1023/A:1026343831909

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