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Transgene integration, organization and interaction in plants

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Abstract

It has been appreciated for many years that the structure of a transgene locus can have a major influence on the level and stability of transgene expression. Until recently, however, it has been common practice to discard plant lines with poor or unstable expression levels in favor of those with practical uses. In the last few years, an increasing number of experiments have been carried out with the primary aim of characterizing transgene loci and studying the fundamental links between locus structure and expression. Cereals have been at the forefront of this research because molecular, genetic and cytogenetic analysis can be carried out in parallel to examine transgene loci in detail. This review discusses what is known about the structure and organization of transgene loci in cereals, both at the molecular and cytogenetic levels. In the latter case, important links are beginning to be revealed between higher order locus organization, nuclear architecture, chromatin structure and transgene expression.

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  1. Rexagen Corporation, Genome Centre, Norwich Bio-Incubator, Colney Lane, Norwich, NR4 7UH, United Kingdom

    Ajay Kohli

  2. Department of Biology, University of York, Heslington, York, YO10 5DD, United Kingdom

    Richard M. Twyman

  3. Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, United Kingdom

    Rita Abranches & Eva Wegel

  4. Biologie VII, RWTH Aachen, Worringerweg 1, 52074, Aachen, Germany

    Eva Stoger

  5. Fraunhofer Institute for Molecular Biology and Applied Ecology, Auf dem Aberg 1, D-57392, Schmallenberg, Germany

    Paul Christou

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Kohli, A., Twyman, R.M., Abranches, R. et al. Transgene integration, organization and interaction in plants. Plant Mol Biol 52, 247–258 (2003). https://doi.org/10.1023/A:1023941407376

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