Abstract
A mapping population of 96 BC1F9lines (Backcross Inbred Lines: BILs),derived by a single-seed descent method rom a backcross of Nipponbare (japonica) / Kasalath (indica // Nippon are, was used to detect quantitative trait loci (QTLs) for leaf bronzing index (LBI), stem dry weight (SDW), tiller number (TN) and root dry weight (RDW) under Fe2+ stress condition in rice. Two parents and 96 BILs were phenotyped for the traits by growing them in Fe2+ toxicity nutrient solution. A total of four QTLs were detected on chromosome 1 and 3, respectively, with LOD of QTLs ranging from 3.17 to 7.03. One QTL controlling LBI, DW, N and RDW was located at the region of C955-C885 on chromosome 1, and their contributions to whole variation were 20.5%, 36.9%, 43.9% and 38.8%,respectively. The QTL located at the region of C955-C885 on chromosome 1 may be important to ferrous iron toxicity tolerance in rice. Another QTL for SDW and RDW was located at the region of C25-C515 on chromosome 3, with respective contributions of 47.9% and 35.0% to whole variation. Further, two QTLs on chromosome 1 were located for RDW at the region of R2329-R210 and for TN at the region of R1928-C178. Comparing with the other mapping results, the QTL located at the region of C955-C885 on chromosome 1 was identical with the results reported previously. There is a linkage between a TL detected under Fe2+ stress condition for stem and root dry weight and a QTL detected under phosphorus-deficiency condition for dry weight on chromosome 3.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bode, K., O. Doring, S. Luthje, H.U. Neue & M. Bottger, 1995. The role of active oxygen in iron tolerance of rice, Oryza sativa L. Protoplasm 184: 249-255.
Conklin, P.L. & R.L. Last, 1995. Differential accumulation of antioxidant mRNA in Arabidopsis thaniana exposed to ozone. Plant Physiol 109: 203-209.
Ezaki, B., S. Tsugita & H. Matsumoyo, 1996. Express of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: Possible involvement of peroxidase isozymes in aluminum ion stress. Plant Physiol 96: 21-28.
Fageria, N.K. & N.A. Rabelo, 1987. Tolerance of rice cultivars to iron toxicity. J Plant Nutr 10: 653-661.
Gunawarkena I., S.S. Virmani & F.J. Sumo, 1982. Breeding rice for tolerance to iron toxicity. Oryza 19: 5-12.
International Rice Research Institute (IRRI), 1965. Annual report 1964 Los Banos, Philippines, pp. 335.
Ishimaru, K., M. Yano, N. Aoki, K. Ono, T. Hirose, S.Y. Lin, L. Monna, T. Sasaki & R. Ohsugi, 2001. Toward the mapping of physiological and agronomic characters on a rice function map: QTL analysis and comparison between QTLs and expressed sequences tags. Theor Appl Genet 102: 793-800.
Li, D.M., J.J. Tang, J.L. Zhou & S.Q. Li, 1991. The Ecophysiological Mechanism of Rice Tolerance for Gleyic Soil Stress and The Breeding of Varieties Tolerance for Soil-related Stress. Rice Review and Abstracts 10(2): 1-4.
Lin, S.Y., T. Sasaki & M. Yano, 1998. Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L. Using backcross inbred lines. Theor Appl Genet 96: 997-1003.
Lincoln, S.E., M.J. Daly & E.S. Lander, 1993. Mapping genes controlling quantitative traits using MAPMAKER/QTL version 1.1: a tutorial and reference manual. 2nd ed. Cambridge Mass, Whitehead Institute for Biometrical Research.
Mahadavappa M., IRRPS, 1979. NO. 43. International Rice Research Institute.
Ming, F., X. Zheng, G. Mi, P. He, L. Zhu & F. Zhang, 1999. Molecular markers links to genes underlying rice seeding tolerance for P-deficiency. Chinese Sci Bull 44(23): 2514-2518.
Ota, Y., 1968. Studies on the occurrence of the physiological disease called ‘bronzing’. Bull Natl Inst Agric Sci (Japan) D. 18: 31-104.
Ponnamperuma, F.N., R. Bradfield & M. Peech, 1955. Physiological disease of rice attributable to iron toxicity. Nature 175: 265.
Smirnoff, N. & S.V. Colombe, 1988. Drought influence the activity of enzymes of the chloroplast hydrogen peroxide scavenging system. J Exp Bot 39: 1097-1108.
Wissuwa, M., M. Yano & N. Ae, 1998. Mapping of QTLs for phosphorus-deficiency tolerance in rice, Oryza sativa L. Theor Appl Genet 97: 777-783.
Wu, P., B. Hu, C. Liao, J. Zhu, Y. Wu, D. Senadhira & A.H. Paterson, 1998. Characterization of tissue tolerance to iron by molecular markers in different lines of rice. Plant and Soil 203: 217-226.
Wu, P., A. Luo & J. Zhu, 1997. Molecular markers links to genes underlying seeding tolerance for ferrous iron toxicity. Plant and Soil 196(2): 317-320.
Wu, P., J. Ni, A. Luo, G. Jin & Q. Tao, 1997. Investigation of QTLs underlying rice tolerance for potassium deficiency via molecular markers. Plant Nutr Fert Sci 3(3): 209-217.
Yan, J., J. Zhu, C. He, M. Benmoussa & P. Wu, 1998. Quantitative trait loci analysis for the developmental behavior of tiller number in rice (Oryza sativa L.) Theor Appl Genet 97: 267-274.
Yoshida, S., 1981. Fundamentals of Rice Crop Science. International Rice Research Institute.
Yoshida, S., D.A. Forno, J.H. Cock & K.A. Gomez, 1976. Laboratory manual for physiological studies of rice. The third edition. Int Rice Res Inst Manila, Philippines, pp. 61-64.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wan, Jl., Zhai, Hq., Wan, Jm. et al. Detection and analysis of QTLs for ferrous iron toxicity tolerance in rice, Oryza sativa L.. Euphytica 131, 201–206 (2003). https://doi.org/10.1023/A:1023915710103
Issue Date:
DOI: https://doi.org/10.1023/A:1023915710103