Abstract
The effects of induced subclinical hypocalcaemia (SCHC) on feed intake and chewing activity during eating and rumination were studied in dairy cows. Two non-lactating and non-pregnant cows were subjected to three different treatments, with one test per day, such that the plasma free (ionized) calcium (FCa) concentration was maintained at the eucalcaemic level or at one of two constant SCHC levels. The cows and test days followed a 2×3 crossover design. SCHC was maintained for 7 h by repeatedly infusing 5% Na2EDTA so that constant FCa concentrations of 0.8 mmol/L or 0.6 mmol/L in plasma were achieved. Control conditions were achieved by infusing isotonic saline. Feed intake and the number of the rumination periods were recorded during test days. The proportion of feed eaten during each test meal (EatPro) was related to the mean plasma FCa. An almost linear decrease in EatPro was observed when the plasma FCa was 0.6–0.9 mmol/L. The cows showed no other clinical signs of hypocalcaemia during Na2EDTA-infusion. The time spent chewing during eating and rumination, and the number of rumination periods during a test day, decreased with a decline in plasma FCa concentration.
It was concluded that induced SCHC depresses the feed intake and ruminative activity of dairy cows.
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REFERENCES
AOAC (Association of Official Analytical Chemists), 1990. Official Methods of Analysis of the Association of Official Analytical Chemists, 15th edn, (Association of Official Chemists, Washington, DC), 1, 8-19, 69, 777
Ballantine, H.T. and Herbein, J.H.P., 1991. Potentiometric determination of ionized and total calcium in blood plasma of Holstein and Jersey cows. Journal of Dairy Science, 74, 446-449
Bertics, S.J., Grummer, R.R., Cadorniga-Valino, C. and Stoddard, E.E., 1992. Effect of prepartum dry matter intake on liver triglyceride concentration and early lactation. Journal of Dairy Science, 75, 1914-1922
Bowen, J.M., Blackmon, D.M. and Heavner, J.E., 1970. Neuromuscular transmission and hypocalcemic paresis in the cow. American Journal of Veterinary Research, 31, 831-839
Brydl, E., 1995. Prevention of metabolic disorders of dairy cows by multi-phase feeding strategy during the peripartal period. Hungarian Veterinary Journal, 50, 718-720
Christensen, R., 1996. Analysis of Variance, Design and Regression: Applied Statistical Methods, (Chapman and Hall, London, UK), 271-279
Coppock, C.E., Noller, C.H., Wolfe, S.A., Calhan, C.J. and Baker, J.S., 1972. Effect of forage:concentrate ratio in complete feeds fed ad libitum on feed intake prepartum and the occurrence of abomasal displacement in dairy cows. Journal of Dairy Science, 55, 783-789
Daniel, R.C.W., 1983. Motility of the rumen and abomasum during hypocalcaemia. Canadian Journal of Comparative Medicine, 47, 276-280
Desmecht, D.J.-M., Linden, A.S., Godeau, J.-M. and Lekeux, P.M., 1995. Experimental production of hypocalcaemia by EDTA infusion in calves: a critical appraisal assessed from the profile of blood chemicals and enzymes. Comparative Biochemistry and Physiology, 110A, 115-130
Endres, D.B. and Rude, R.K., 1996. Mineral and bone metabolism. In: C.A. Burtis and R. Ashwood (eds), Tietz Fundamentals of Clinical Chemistry, 4th edn, (W.B. Saunders, Philadelphia, PA), 685-689
Fenwick, D.C. and Daniel, R.C.W., 1990. A comparison between the main clinical signs of milk fever in cows with those of hypocalcaemia induced by Na2EDTA solution. Journal of Veterinary Medicine, Series A, 37, 721-728
Goff, J.P. and Horst, R., 1997. Physiological changes at parturition and their relationship to metabolic disorders. Journal of Dairy Science, 80, 1260-1268.
GraphPad Software, Inc., 5755 Oberlin Drive, 110 San Diego, CA 92121, USA. Homepage: http://www.graphpad.com
Grummer, R.R., Bertics, S.J., LaCount, D.W., Snow, J.A., Dentine, M.R. and Stauffacher, R.H., 1990. Estrogen induction of fatty liver in dairy cattle. Journal of Dairy Science, 73, 1537-1543
Hansen, S.S., Jensen, A.L. and Jørgensen, R.J., 2000. Evaluation of a transportable [Ca2+] and pH analyser and of the impact of different anticoagulants and sampling sites in cattle. Journal of Veterinary Medicine, Series A, 47, 541-551
Hansen, S.S., Mellau, L.S.B., Jørgensen, R.J., Enemark, J.D., Nørgaard, P. and Pedersen, C., 2001. Induction of hypocalcaemia clamps attempted by controlled infusion with Na2EDTA in dairy cows. In: Meeting Documents from Abildgaard Symposium, The Abildgaard Foundation at KVL and The Danish Agricultural and Veterinary Research Council. 16–17 August, Copenhagen, Denmark, 201-202
Herdt, T., 1997. Digestion: The fermentative processes. In: J.G. Cunningham (ed.), Textbook of Veterinary Physiology, (W.B. Saunders, Philadelphia), 331-359
Hove, K., 1986. Cyclic changes in plasma calcium and the calcium homeostatic endocrine system of the postparturient dairy cow. Journal of Dairy Science, 69, 2072-2082
Jonsson, N.N., Pepper, P.M., Daniel, R.C.W., McGowan, M.R. and Fulkerson, W., 1999. Association between non-parturient post-partum hypocalcaemia and the interval from calving to first ovulation in Holstein-Friesian cows. Animal Science, 68, 377-383
Jørgensen, R.J., Nyengaard, N.R., Hara, S., Enemark, J.D. and Andersen, P.H., 1998. Rumen motility during induced hyper-and hypocalcaemia. Acta Veterinaria Scandinavica, 39, 331-338
Jørgensen, R.J., Nyengaard, N.R., Daniel, R.C.W., Mellau, L.S.B. and Enemark, J.M., 1999. Induced hypocalcaemia by Na2EDTA infusion. A review. Journal of Veterinary Medicine, Series A, 46, 389-407
Kamgarpour, R., Daniel, R.C.W., Fenwick, D.C., McGuigan, K. and Murphy, G., 1999. Post partum subclinical hypocalcaemia and effects on ovarian function and uterine involution in a dairy herd. The Veterinary Journal, 158, 59-67
King, R.D., 1984. Developments in Food Analysis Techniques, vol. 1 (Applied Science, London), 293-314
Kvart, C. and Larsson, L., 1978. Studies on ionized calcium in serum and plasma from normal cows. Its relation to total serum calcium and the effects of sample storing. Acta Veterinaria Scandinavica, 19, 487-496
Kvart, C., Larsson, L. and Björsell, A., 1980. Influence of an electrode change on reference values for cow serum ionized calcium. Acta Veterinaria Scandinavica, 21, 454-456
Kvart, C., Björsell, K.A. and Larsson, L., 1982. Parturient paresis in the cow. Serum ionized calcium concentrations before and after treatment with different calcium solutions — classification of different degrees of hypo-and hypercalcemia. Acta Veterinaria Scandinavica, 32, 184-196
Larsson, L., Björsell, K.A., Kvart, C. and Öhman, S., 1983. Clinical signs and serum ionized calcium in parturient paretic cows. Zentalblatt für Veterinärmedicine, reihe A, 30, 401-409
Lincoln, S.D. and Lane, V.M., 1990. Serum ionized calcium concentration in clinically normal dairy cattle, and changes associated with calcium abnormalities. Journal of American Veterinary Medical Association, 197, 1471-1474
Marquardt, J.P., Horst, R.L. and Jørgensen, N.A., 1977. Effect of parity on dry matter intake at parturition in dairy cattle. Journal of Dairy Science, 60, 929-934
McKay, B., 1994. Subclinical hypocalcaemia: a possible affect on fertility. In: Proceedings of the 11th seminar for the Society of Dairy Cattle Veterinarians of the New Zealand Veterinary Association, Queenstown, New Zealand, 89-98
Oetzel, G., 1996. Improving reproductive performance in dairy cattle via milk fever prevention. The Bovine Proceedings, 28, 52-59
Oetzel, G. and Berger, L.L., 1985. Protein-energy malnutrition in domestic ruminants. Part 1. Predisposing factors and pathophysiology. Compendium on Continuing Education, 7, 672-679
Schleisner, C., Nørgaard, P. and Hansen, H.H., 1999. Discriminant analysis of patterns of jaw movements during rumination and eating in a cow. Acta Agriculturae Scandinavica, Section A, Animal Science, 49, 251-259
Studer, V.A., Grummer, R.R., Bertics, S.J. and Reynolds, C.K., 1993. Effect of prepartum propylene glycol administration on periparturient fatty liver in dairy cows. Journal of Dairy Science, 76, 2931-2939
Stuffins, C.B., 1967. The determination of phosphate and calcium in feeding stuffs. The Analyst, 92, 107-111
Tecator, 1978. Determination of crude fibre in some feed and food samples by using the fibertec system and Wende method. Application Note 1. Tecator AB, Box 70, S-26301 Hoganæs, Sweden
Tilley, J.M.A. and Terry, R.A., 1963. A two-stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society, 18, 104-111
Van Soest, P.J., 1963. Use of detergents in the analysis of fibrous feeds. Journal of the Association of Official Agricultural Chemists, 46, 825-835
Vasquez-Anon, M.S., Bertics, S., Luck, M. and Grummer, R.R., 1994. Prepartum liver triglyceride and plasma metabolites in dairy cows. Journal of Dairy Science, 77, 1521-1528
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Hansen, S., Nørgaard, P., Pedersen, C. et al. The Effect of Subclinical Hypocalcaemia Induced by Na2EDTA on the Feed Intake and Chewing Activity of Dairy Cows. Vet Res Commun 27, 193–205 (2003). https://doi.org/10.1023/A:1023340506782
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DOI: https://doi.org/10.1023/A:1023340506782