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Volatile Compounds Released by Disturbed and Calm Adults of the Tarnished Plant Bug, Lygus lineolaris

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Abstract

Volatile compounds released by disturbed and calm female and male Lygus lineolaris were collected and analyzed. Six major compounds were present in samples from disturbed bugs and from calm females: (E)-2-hexenal, 1-hexanol, (E)-2-hexenol, hexyl butyrate, (E)-2-hexenyl butyrate, and (E)-2,4-oxohexenal. (E)-2-hexenal was lacking in volatiles collected from calm males. Hexyl butyrate accounted for approximately 68% and 66% of volatiles released by agitated and calm females, and 87% and 88% of volatiles released by agitated and calm males, respectively. Blends released by disturbed insects differed quantitatively from blends released by calm insects, with amounts of compounds increasing 75–350 times in samples from disturbed insects. In static air bioassays, both females and males were repelled by natural volatiles collected from females and by five-component [(E)-2,4-oxohexenal excluded] and six-component synthetic blends at doses of 1 and 10 bug-hours, indicating that these volatiles may serve an alarm or epideictic function, as well as a possible role as defensive allomones. Adults also avoided hexyl butyrate, (E)-2-hexenyl butyrate, (E)-2-hexenol, and (E)-2,4-oxohexenal, but not 1-hexanol and (E)-2-hexenal when compounds were assayed individually in static air bioassays at doses equal to 1 bug-hour. When tested over 1 day in two-choice cage trials, adults did not prefer untreated bean plants over bean plants surrounded by vials releasing up to 8.1 mg/hr (=234 bug-hours) of the five-component synthetic blend. Therefore, the volatiles produced by disturbed adults would not be useful as a repellent for L. lineolaris.

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References

  • Aldrich, J. R. 1988. Chemical ecology of the Heteroptera. Annu. Rev. Entomol. 33:211–238.

    Google Scholar 

  • Aldrich, J. R., Lusby, W. R., Kochansky, J. P., Hoffmann, M. P., Wilson, L. T., and Zalom, F. G. 1988. Lygus bug pheromones vis-à-vis stink bugs, pp. 213–216, in Proceedings, Beltwide Cotton Production Research Conferences. National Cotton Council, Memphis, Tennessee.

    Google Scholar 

  • Aldrich, J. R., Rosi, M. C., and Bin, F. 1995. Behavioral correlates for minor volatile compounds from stink bugs (Heteroptera: Pentatomidae). J. Chem. Ecol. 21:1907–1920.

    Google Scholar 

  • Blatt, S. E., Borden, J. H., Pierce, H. D., Jr., Gries, R., and Gries, G. 1998. Alarm pheromone system of the western conifer seed bug, Leptoglossus occidentalis. J. Chem. Ecol. 24:1013–1031.

    Google Scholar 

  • Blum, M. S. 1981. Chemical Defences of Arthropods. Academic Press, New York.

    Google Scholar 

  • Blum, M. S. 1985. Alarm pheromones, pp. 193–224, in G.A. Kerkut and L.I. Gilbert (Eds.). Comprehensive Insect Physiology, Biochemistry, and Pharmacology, Vol. 9. Pergamon Press, Oxford.

    Google Scholar 

  • Blumenthal, M. A. 1978. The metathoracic gland system of Lygus lineolaris (Heteroptera: Miridae). MSc. thesis. Cornell University, Ithaca, New York, 142pp.

    Google Scholar 

  • Borden, J. H., Chong, L., and Lindgren, B. S. 1990. Redundancy in the semiochemical message required to induce attack on lodgepole pines by the mountain pine beetle, Dendoctronus ponderosae Hopkins (Coleoptera: Scolytidae). Can. Entomol. 122:769–777.

    Google Scholar 

  • Borden, J. H., Wilson, I. M., Gries, R., Chong, L. J., Pierce, H. D., Jr., and Gries, G. 1998. Volatiles from the bark of trembling aspen, Populus tremuloides Michx. (Salicaceae) disrupt secondary attraction by the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae). Chemoecology 8:69–75.

    Google Scholar 

  • Bryan, D. E., Jackson, C. G., Carranza, R. L., and Neemann, E. G. 1976. Lygus hesperus: Production and development in the laboratory. J. Econ. Entomol. 69:127–129.

    Google Scholar 

  • Butts, R. A. and Lamb, R. J. 1991. Pest status of lygus bugs (Hemiptera: Miridae) in oilseed Brassica crops. J. Econ. Entomol. 84:1591–1596.

    Google Scholar 

  • Chinta, S., Dickens, J. C., and Aldrich, J. R. 1994. Olfactory reception of potential pheromones and plant odors by tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae). J. Chem. Ecol. 20:3251–3267.

    Google Scholar 

  • Conover, W. J. 1980. Practical Nonparametric Statistics, 2nd ed. Wiley, New York.

    Google Scholar 

  • Curtis, C. E. and McCoy, C. E. 1964. Some host-plant preferences shown by Lygus lineolaris (Hemiptera: Miridae) in the laboratory. Ann. Entomol. Soc. Am. 57:511–513.

    Google Scholar 

  • Dawson, G. W., Gibson, R. W., Griffiths, D. C., Pickett, J. A., Rice, A. D., and Woodcock, C. M. 1982. Aphid alarm pheromone derivatives affecting settling and transmission of plant viruses. J. Chem. Ecol. 8:1377–1388.

    Google Scholar 

  • Day, W. H. 1987. Biological control efforts against Lygus and Adelphocoris spp. infesting alfalfa in the United States, with notes on other associated mirid species, pp. 20–39, in R. C. Hedlund and H. M. Graham (Eds.), Economic Importance and Biological Control of Lygus and Adelphocoris in North America. USDA Agricultural Research Service Publication A.R.S.-64. USDA, Washington, D.C.

    Google Scholar 

  • Gueldner, R. C. and Parrott, W. L. 1978. Volatile constituents of the tarnished plant bug. Insect Biochem. 8:389–391.

    Google Scholar 

  • Gunawardena, N. E. and Bandumathie, M. K. 1993. Defensive secretion of rice bug, Leptocorisa oratorius Fabricius, (Hemiptera: Coreidae): A unique chemical combination and its toxic, repellent, and alarm properties. J. Chem. Ecol. 19:851–861.

    Google Scholar 

  • Hardee, D. D. and Bryan, W. W. 1997. Influence of Bacillus thuringiensis-transgenic and nectariless cotton on insect populations with emphasis on the tarnished plant bug (Heteroptera: Miridae). J. Econ. Entomol. 90:663–668.

    Google Scholar 

  • Hardee, D. D. and Herzog, G. A. 1996. 49th Annual conference report on cotton insect research and control, pp. 643–669, in Proceedings, Beltwide Cotton Production Research Conferences. National Cotton Council, Memphis, Tennessee.

    Google Scholar 

  • Hedlund, R. C. and Graham, H. M. (Eds.) 1987. Economic Importance and Biological Control of Lygus and Adelphocoris in North America. USDA Agricultural Research Service Publication A.R.S.-64. USDA, Washington, D.C.

    Google Scholar 

  • Jennings, W. and Shibamoto, T. 1980. Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Academic Press, New York.

    Google Scholar 

  • Kelton, L. A. 1975. The lygus bugs (Genus Lygus Hahn) of North America (Heteroptera: Miridae). Mem. Entomol. Soc. Can. 95:1–101.

    Google Scholar 

  • Krall, B. S., Bartelt, R. J., Lewis, C. J., and Whitman, D. W. 1999. Chemical defense in the stink bug Cosmopepla bimaculata. J. Chem. Ecol. 25:2477–2494.

    Google Scholar 

  • Leal, W. S., Panizzi, A. R., and Niva, C. C. 1994. Alarm pheromone system of leaf-footed bug Leptoglossus zonatus (Heteroptera: Coreidae). J. Chem. Ecol. 20:1209–1216.

    Google Scholar 

  • Lockwood, J. A. and Story, R. N. 1985. Bifunctional pheromone in the first instar of the southern green stink bug, Nezara viridula (L.) (Hemiptera: Pentatomidae): Its characterization and interaction with other stimuli. Ann. Entomol. Soc. Am. 78:474–479.

    Google Scholar 

  • Lockwood, J. A. and Story, R. N. 1987. Defensive secretion of the southern green stink bug (Hemiptera: Pentatomidae) as an alarm pheromone. Ann. Entomol. Soc. Am. 80:686–691.

    Google Scholar 

  • Nault, L. R. and Phelan, P. L. 1984. Alarm pheromones and sociality in pre-social insects, pp. 237–256, in W. J. Bell and R. T. Cardé (Eds.). Chemical Ecology of Insects. Chapman and Hall, London, United Kingdom.

    Google Scholar 

  • Parrott, W. L., Jenkins, J. N., Maxwell, F. G., and Bostick, M. L. 1975. Improved techniques for rearing the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). Miss. Agric. For. Exp. Stn. Tech. Bull. 72.

  • Pavis, C., Malosse, C., Ducrot, P. H., and Descoins, C. 1994. Dorsal abdominal glands in nymphs of southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae): Chemistry of secretions of five instars and role of (E)-4-oxo-2-decenal, compound specific to first instars. J. Chem. Ecol. 20:2213–2227.

    Google Scholar 

  • Piancatelli, G., Scettri, A., and D'Auria, M. 1980. Oxidative ring opening of furan derivatives to α,β-unsaturated γ-dicarbonyl compounds, useful intermediates for 3-oxocyclopentenes synthesis. Tetrahedron 36:661–663.

    Google Scholar 

  • Prokopy, R. J. 1981. Epideictic pheromones that influence spacing patterns of phytophagous insects, pp. 181–213, in D. A. Nordlund, R. L. Jones and W. J. Lewis (Eds.). Semiochemicals: Their Role in Pest Control. Wiley, New York.

    Google Scholar 

  • Prokopy, R. J., Coli, W. M., Hislop, R. G., and Hauschild, K. I. 1980. Integrated management of insect and mite pests in commercial apple orchards in Massachusetts. J. Econ. Entomol. 73:529–535.

    Google Scholar 

  • Schaefers, G. A. and Shanks, C. H., Jr. 1991. Pest management for strawberry insects, pp. 535–552, in D. Pimentel (ed.). C.R.C. Handbook of Pest Management in Agriculture, 2nd ed., Vol. III. C.R.C. Press, Boca Raton, Florida.

    Google Scholar 

  • Slaymaker, P. H. and Tugwell, N. P. 1982. Low-labor method for rearing the tarnished plant bug (Hemiptera: Miridae). J. Econ. Entomol. 75:487–488.

    Google Scholar 

  • Snodgrass, G. L. 1996. Insecticide resistance in field populations of the tarnished plant bug (Heteroptera: Miridae) in cotton in the Mississippi Delta. J. Econ. Entomol. 89:783–790.

    Google Scholar 

  • Staddon, B. W. 1979. The scent glands of Heteroptera. Adv. Insect Physiol. 14:351–418.

    Google Scholar 

  • Staples, J. K., Krall, B. S., Bartelt, R. J., and Whitman, D. W. 2002. Chemical defense in the plant bug Lopidea robiniae (Uhler). J. Chem. Ecol. 28:601–615.

    Google Scholar 

  • Teerling, C. R., Pierce, H. D., Jr., Borden, J. H., and Gillespie, D. R. 1993. Identification and bioactivity of alarm pheromone in the western flower thrips, Frankliniella occidentalis. J. Chem. Ecol. 19:681–697.

    Google Scholar 

  • Vet, L. E. M. 1983. Host-habitat location through olfactory cues by Leptopilina clavipes (Hartig) (Hym.: Eucoilidae), a parasitoid of fungivorous Drosophila: The influence of conditioning. Neth. J. Zool. 33:225–248.

    Google Scholar 

  • Walstrom, R. J. 1983. Plant bug (Heteroptera: Miridae) damage to first-crop alfalfa in South Dakota. J. Econ. Entomol. 76:1309–1311.

    Google Scholar 

  • Wohlers, P. 1981a. Effects of the alarm pheromone (E)-β-farnesene on dispersal behaviour of the pea aphid Acyrthosiphon pisum. Entomol. Exp. Appl. 29:117–124.

    Google Scholar 

  • Wohlers, P. 1981b. Aphid avoidance of plants contaminated with alarm pheromone (E)-β-farnesene. Z. Angew. Entomol. 92:329–336.

    Google Scholar 

  • Young, O. P. 1986. Host plants of the tarnished plant bug, Lygus lineolaris (Heteroptera: Miridae). Ann. Entomol. Soc. Am. 79:747–762.

    Google Scholar 

  • Zar, J. H. 1984. Biostatistical Analysis, 2nd ed. Prentice-Hall, Englewood Cliffs, New Jersey.

    Google Scholar 

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Wardle, A.R., Borden, J.H., Pierce, H.D. et al. Volatile Compounds Released by Disturbed and Calm Adults of the Tarnished Plant Bug, Lygus lineolaris . J Chem Ecol 29, 931–944 (2003). https://doi.org/10.1023/A:1022987901330

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